Restriction map for
pFN21AE1781
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AgeI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MaeI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 269 GGTACC G^GTACC
AflIII 257 ACATGT A^C[GA][CT]GT
AhdI 695 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 393 GATCC GGATC(4/5)
ApaI 445 GGGCCC GGGCC^C
Asp700I 43 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 269 GGTACC G^GTACC
AspEI 695 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 836 TGGCCA TGG^CCA
BciVI 10 GGATAC GTATCC(6/5)
BglII 137 AGATCT A^GATCT
BpmI 754 CTGGAG CTGGAG(16/14)
BsaJI 329 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 500 ACCGGA [AT]^CCGG[AT]
BsgI 800 CTGCAC GTGCAG(16/14)
BsiEI 498 CGACCG CG[GA][CT]^CG
BsmI 842 GCATTC GAATGC(1/-1)
BspLU11I 257 ACATGT A^CATGT
BspMI 248 GCAGGT ACCTGC(4/8)
BstXI 827 CCATTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 137 AGATCT [GA]^GATC[CT]
Eam1105I 695 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 382 GATATC GAT^ATC
HapII 501 CCGG C^CGG
HgaI 643 GACGC GACGC(5/10)
HinfI 701 GAGTC G^A[ACGT]TC
HpaII 501 CCGG C^CGG
KpnI 269 GGTACC GGTAC^C
MaeII 552 ACGT A^CGT
MflI 137 AGATCT [GA]^GATC[CT]
MluNI 836 TGGCCA TGG^CCA
MscI 836 TGGCCA TGG^CCA
MseI 124 TTAA T^TAA
MspA1I 785 CAGCTG C[AC]G^C[GT]G
MspI 501 CCGG C^CGG
NspI 257 ACATGT [GA]CATG^[CT]
PflMI 309 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 701 GAGTC GAGTC(4/5)
PvuII 785 CAGCTG CAG^CTG
SexAI 414 ACCTGGT A^CC[AT]GGT
SmlI 74 CTCAAG C^T[CT][GA]AG
TaqI 699 TCGA T^CGA
Tru9I 124 TTAA T^TAA
TthHB8I 699 TCGA T^CGA
Van91I 309 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 137 AGATCT [GA]^GATC[CT]
XmnI 43 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BanI 269 GGTACC G^G[CT][GA]CC
568 GGCACC
BanII 445 GGGCCC G[GA]GC[CT]^C
518 GGGCTC
BmyI 445 GGGCCC G[AGT]GC[ACT]^C
518 GGGCTC
Bsp1286I 445 GGGCCC G[AGT]GC[ACT]^C
518 GGGCTC
EaeI 195 TGGCCG [CT]^GGCC[GA]
836 TGGCCA
EcoNI 436 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
753 CCTGGAGAAGG
MboII 22 GAAGA GAAGA(8/7)
772 GAAGA
MwoI 549 GCAACGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
624 GCTGCAGAAGC
PstI 38 CTGCAG CTGCA^G
625 CTGCAG
SfaNI 240 GATGC GCATC(5/9)
490 GATGC
Tsp509I 122 AATT ^AATT
809 AATT
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
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AfaI AluI AlwNI BbvI BsiYI
BslI BsmAI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HphI
ItaI MaeIII MboI MnlI MslI
MvaI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp45I TspRI