Restriction map for
pFN21AB9648
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BseRI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrI BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FokI FseI FspI
HindIII HinfI HpaI KpnI Ksp632I
MaeI MaeIII MamI MboII MfeI
MflI MluI MluNI MroI MscI
MseI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI SexAI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I TspRI XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 35 enzymes) #
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AluI 629 AGCT AG^CT
AlwNI 502 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 69 GGGCCC GGGCC^C
Asp700I 165 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 340 GAGCAC G[AT]GC[AT]^C
AspI 145 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 1028 TGATCA T^GATCA
BpmI 508 CTGGAG CTGGAG(16/14)
BsaI 19 GAGACC GGTCTC(1/5)
BsiHKAI 340 GAGCAC G[AT]GC[AT]^C
BsmAI 19 GAGAC GTCTC(1/5)
Bsp1407I 2 TGTACA T^GTACA
BsrGI 2 TGTACA T^GTACA
BstXI 461 CCATGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI 1028 TGATCA T^GATCA
HincII 550 GTCAAC GT[CT]^[GA]AC
HindII 550 GTCAAC GT[CT]^[GA]AC
HphI 951 GGTGA GGTGA(8/7)
MaeII 548 ACGT A^CGT
NcoI 461 CCATGG C^CATGG
NspI 1034 GCATGT [GA]CATG^[CT]
PflFI 145 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 501 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1013 GGGACCT [GA]G^G[AT]CC[CT]
PstI 760 CTGCAG CTGCA^G
PvuII 628 CAGCTG CAG^CTG
ScaI 401 AGTACT AGT^ACT
SgrAI 1047 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 388 CTCAAG C^T[CT][GA]AG
SspBI 2 TGTACA T^GTACA
TaqI 302 TCGA T^CGA
Tth111I 145 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 302 TCGA T^CGA
Van91I 501 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 165 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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BglI 515 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
886 GCCGTGGCGGC
BsmFI 1013 GGGAC GGGAC(10/14)
183 GTCCC
BspMI 26 ACCTGC ACCTGC(4/8)
1016 ACCTGC
BstAPI 729 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1104 GCACTCGCTGC
DpnII 240 GATC ^GATC
1029 GATC
EcoT14I 131 CCAAGG C^C[AT][AT]GG
461 CCATGG
MboI 240 GATC ^GATC
1029 GATC
MslI 9 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
462 CATGGGCGTG
NdeII 240 GATC ^GATC
1029 GATC
Sau3AI 240 GATC ^GATC
1029 GATC
SfaNI 225 GATGC GCATC(5/9)
615 GATGC
SfcI 455 CTGTAG C^T[GA][CT]AG
760 CTGCAG
SmaI 648 CCCGGG CCC^GGG
1009 CCCGGG
StyI 131 CCAAGG C^C[AT][AT]GG
461 CCATGG
XmaCI 648 CCCGGG C^CCGGG
1009 CCCGGG
XmaI 648 CCCGGG C^CCGGG
1009 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AfaI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BsgI
BsiEI BsiYI BslI BsoBI Bsp1286I
BsrFI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI KasI KspI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NgoMIV
NlaIII NlaIV NotI RsaI SacII
Sau96I ScrFI SfoI TseI