Restriction map for
pFN21AB3007
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AviII
BanI BbeI BbrPI BbvCI BcgI
BclI BglI BlpI Bpu1102I BsaHI
BsiEI BsiWI BsmBI BsmI BspDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FbaI
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MluI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XcmI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 1665 TCCGGA T^CCGGA
AlwNI 2888 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1253 GGGCCC GGGCC^C
AseI 1033 ATTAAT AT^TAAT
AsnI 1033 ATTAAT AT^TAAT
Asp700I 2158 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 144 CCCGGG C^[CT]CG[GA]G
AvrII 2172 CCTAGG C^CTAGG
BlnI 2172 CCTAGG C^CTAGG
BsaAI 1790 TACGTG [CT]AC^GT[GA]
BsaBI 2611 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1665 TCCGGA T^CCGGA
BsgI 1625 CTGCAC GTGCAG(16/14)
BsoBI 144 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1953 TGTACA T^GTACA
BspEI 1665 TCCGGA T^CCGGA
BspLU11I 798 ACATGT A^CATGT
BsrBI 1873 CCGCTC CCGCTC(-3/-3)
BsrGI 1953 TGTACA T^GTACA
BstBI 1976 TTCGAA TT^CGAA
DraIII 424 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 130 GACACGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2113 GAATTC G^AATTC
EcoRV 196 GATATC GAT^ATC
FauI 105 GCGGG CCCGC(4/6)
HgaI 1323 GACGC GACGC(5/10)
HindIII 2159 AAGCTT A^AGCTT
MamI 2611 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1665 TCCGGA T^CCGGA
NcoI 2256 CCATGG C^CATGG
NspV 1976 TTCGAA TT^CGAA
PshBI 1033 ATTAAT AT^TAAT
SalI 139 GTCGAC G^TCGAC
SexAI 1965 ACCTGGT A^CC[AT]GGT
SfuI 1976 TTCGAA TT^CGAA
SmaI 144 CCCGGG CCC^GGG
SphI 158 GCATGC GCATG^C
SspBI 1953 TGTACA T^GTACA
XmaCI 144 CCCGGG C^CCGGG
XmaI 144 CCCGGG C^CCGGG
XmnI 2158 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflII 289 CTTAAG C^TTAAG
1809 CTTAAG
AflIII 798 ACATGT A^C[GA][CT]GT
1791 ACGTGT
BalI 2 TGGCCA TGG^CCA
1003 TGGCCA
BamHI 1797 GGATCC G^GATCC
2911 GGATCC
BciVI 383 GGATAC GTATCC(6/5)
3723 GTATCC
BfrI 289 CTTAAG C^TTAAG
1809 CTTAAG
BglII 2346 AGATCT A^GATCT
3078 AGATCT
BpmI 615 CTGGAG CTGGAG(16/14)
2641 CTGGAG
BsaI 58 GAGACC GGTCTC(1/5)
40 GGTCTC
BsaWI 61 ACCGGA [AT]^CCGG[AT]
1665 TCCGGA
BspHI 1763 TCATGA T^CATGA
2766 TCATGA
BstXI 690 CCAAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1748 CCAGCCATTTGG
CfoI 1276 GCGC GCG^C
2391 GCGC
DraI 1960 TTTAAA TTT^AAA
3048 TTTAAA
DsaI 1590 CCGTGG C^C[GA][CT]GG
2256 CCATGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
1003 TGGCCA
EcoNI 1861 CCTAACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2167 CCTGTCCTAGG
HhaI 1276 GCGC GCG^C
2391 GCGC
HinP1I 1276 GCGC G^CGC
2391 GCGC
HphI 259 GGTGA GGTGA(8/7)
889 GGTGA
MaeII 1791 ACGT A^CGT
3167 ACGT
MfeI 2186 CAATTG C^AATTG
3184 CAATTG
MluNI 2 TGGCCA TGG^CCA
1003 TGGCCA
MscI 2 TGGCCA TGG^CCA
1003 TGGCCA
MunI 2186 CAATTG C^AATTG
3184 CAATTG
PflMI 250 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1006 CCAAATTCTGG
PstI 1454 CTGCAG CTGCA^G
1895 CTGCAG
PvuII 713 CAGCTG CAG^CTG
1327 CAGCTG
RcaI 1763 TCATGA T^CATGA
2766 TCATGA
SacI 785 GAGCTC GAGCT^C
1439 GAGCTC
Van91I 250 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1006 CCAAATTCTGG
XbaI 1231 TCTAGA T^CTAGA
1443 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PpuMI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII