##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tsp45I      Tsp509I     TspRI       Tth111I     
Van91I      XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII         310  GACGTC               GACGT^C
AciI           71  CCGC                 CCGC(-3/-1)
AcyI          310  GACGTC               G[GA]^CG[CT]C
AfaI          325  GTAC                 GT^AC
BanI          162  GGCACC               G^G[CT][GA]CC
BanII          41  GAGCCC               G[GA]GC[CT]^C
BbsI          139  GAAGAC               GAAGAC(2/6)
BbvI          115  GCAGC                GCAGC(8/12)
BglI          154  GCCAGACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         267  AGATCT               A^GATCT
BmyI           41  GAGCCC               G[AGT]GC[ACT]^C
BpuAI         139  GAAGAC               GAAGAC(2/6)
BsaHI         310  GACGTC               G[GA]^CG[CT]C
BseRI          16  CTCCTC               GAGGAG(10/8)
BsmFI         234  GGGAC                GGGAC(10/14)
Bsp1286I       41  GAGCCC               G[AGT]GC[ACT]^C
BspHI           8  TCATGA               T^CATGA
BspMI         133  GCAGGT               ACCTGC(4/8)
BstXI         188  CCAGCCTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          321  CTCAG                C^T[ACGT]AG
EarI           19  CTCTTC               CTCTTC(1/4)
EcoT14I       172  CCAAGG               C^C[AT][AT]GG
Hin1I         310  GACGTC               G[GA]^CG[CT]C
HphI          136  GGTGA                GGTGA(8/7)
Ksp632I        19  CTCTTC               CTCTTC(1/4)
MseI          305  TTAA                 T^TAA
RcaI            8  TCATGA               T^CATGA
RsaI          325  GTAC                 GT^AC
ScaI          324  AGTACT               AGT^ACT
StuI          204  AGGCCT               AGG^CCT
StyI          172  CCAAGG               C^C[AT][AT]GG
TaqI          263  TCGA                 T^CGA
Tru9I         305  TTAA                 T^TAA
TseI          115  GCAGC                G^C[AT]GC
TthHB8I       263  TCGA                 T^CGA
XbaI          270  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BsiYI          81  CCCAGGATCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               82  CCAGGATCGGG         
BslI           81  CCCAGGATCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               82  CCAGGATCGGG         
BstYI         184  AGATCC               [GA]^GATC[CT]
              267  AGATCT              
Fnu4HI         70  GCCGC                GC^[ACGT]GC
              115  GCAGC               
HaeIII         69  GGCC                 GG^CC
              205  GGCC                
ItaI           70  GCCGC                GC^[ACGT]GC
              115  GCAGC               
MaeII         302  ACGT                 A^CGT
              311  ACGT                
MflI          184  AGATCC               [GA]^GATC[CT]
              267  AGATCT              
SfaNI         256  GATGC                GCATC(5/9)
              109  GCATC               
XhoII         184  AGATCC               [GA]^GATC[CT]
              267  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AluI        AlwI        BfaI        BsaJI       BssKI       
BstNI       DpnII       EcoRII      HinfI       MaeI        
MboI        MboII       MnlI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
ScrFI