Restriction map for
pFN21AE1874
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AhdI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BamHI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BseAI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrGI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI DpnII DraI DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MboI MfeI MflI MluI MroI
MseI MunI NcoI NdeI NdeII
NheI NotI NruI NspV PacI
PflMI PmeI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI SacI
SalI Sau3AI SbfI ScaI SfcI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 520 GTCTAC GT^[AC][GT]AC
AfaI 1146 GTAC GT^AC
AgeI 861 ACCGGT A^CCGGT
Aor51HI 215 AGCGCT AGC^GCT
AscI 486 GGCGCGCC GG^CGCGCC
AspI 514 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 827 CTCGAG C^[CT]CG[GA]G
BalI 329 TGGCCA TGG^CCA
BanII 207 GGGCTC G[GA]GC[CT]^C
BbrPI 547 CACGTG CAC^GTG
BfaI 1096 CTAG C^TAG
BglI 940 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1166 GCTTAGC GC^T[ACGT]AGC
BpmI 1039 CTGGAG CTGGAG(16/14)
Bpu1102I 1166 GCTTAGC GC^T[ACGT]AGC
BsaAI 547 CACGTG [CT]AC^GT[GA]
BsaI 769 GAGACC GGTCTC(1/5)
BsaWI 861 ACCGGT [AT]^CCGG[AT]
BsoBI 827 CTCGAG C^[CT]CG[GA]G
BspLU11I 347 ACATGT A^CATGT
BsrDI 333 CATTGC GCAATG(2/0)
BstXI 1125 CCAGCCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1166 GCTTAGC GC^T[ACGT]AGC
CpoI 578 CGGTCCG CG^G[AT]CCG
DraII 224 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 811 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 680 CGGCCG C^GGCCG
EarI 721 CTCTTC CTCTTC(1/4)
EclXI 680 CGGCCG C^GGCCG
Eco47III 215 AGCGCT AGC^GCT
Eco52I 680 CGGCCG C^GGCCG
EcoO109I 224 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1138 ATGCAT ATGCA^T
Ksp632I 721 CTCTTC CTCTTC(1/4)
KspI 660 CCGCGG CCGC^GG
MaeI 1096 CTAG C^TAG
MluNI 329 TGGCCA TGG^CCA
MscI 329 TGGCCA TGG^CCA
NsiI 1138 ATGCAT ATGCA^T
PaeR7I 827 CTCGAG C^TCGAG
PflFI 514 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 861 ACCGGT A^CCGGT
PmaCI 547 CACGTG CAC^GTG
PmlI 547 CACGTG CAC^GTG
PpuMI 224 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 1146 GTAC GT^AC
RsrII 578 CGGTCCG CG^G[AT]CCG
SacII 660 CCGCGG CCGC^GG
SapI 720 GCTCTTC GCTCTTC(1/4)
SexAI 812 ACCTGGT A^CC[AT]GGT
SphI 1136 GCATGC GCATG^C
TfiI 412 GATTC G^A[AT]TC
Tsp509I 140 AATT ^AATT
Tth111I 514 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 827 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 347 ACATGT A^C[GA][CT]GT
548 ACGTGT
BsgI 655 CTGCAC GTGCAG(16/14)
1087 CTGCAC
BssSI 118 CTCGTG CACGAG(-5/-1)
229 CTCGTG
BstAPI 114 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
138 GCAATTTGTGC
BstF5I 264 GGATG GGATG(2/0)
915 GGATG
DsaI 660 CCGCGG C^C[GA][CT]GG
962 CCGTGG
FokI 264 GGATG GGATG(9/13)
915 GGATG
HgaI 996 GACGC GACGC(5/10)
688 GCGTC
MaeIII 373 GTGAC ^GT[ACGT]AC
1046 GTCAC
MboII 257 TCTTC GAAGA(8/7)
722 TCTTC
MslI 333 CATTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
708 CACGCCGGTG
MspA1I 185 CAGCGG C[AC]G^C[GT]G
660 CCGCGG
NlaIII 348 CATG CATG^
1137 CATG
NspI 347 ACATGT [GA]CATG^[CT]
1136 GCATGC
SfaNI 685 GATGC GCATC(5/9)
916 GATGC
SgrAI 710 CGCCGGTG C[GA]^CCGG[CT]G
860 CACCGGTG
SmlI 827 CTCGAG C^T[CT][GA]AG
1077 CTTGAG
TaqI 512 TCGA T^CGA
828 TCGA
Tsp45I 373 GTGAC ^GT[GC]AC
1046 GTCAC
TthHB8I 512 TCGA T^CGA
828 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BbeI BbvI BcgI BcnI
BmyI BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrFI BsrI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MnlI MspI
MvaI MvnI MwoI NaeI NarI
NciI NgoMIV NlaIV PleI Sau96I
ScrFI SfoI TseI TspRI