Restriction map for
pFN21AE2425
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SalI SbfI ScaI SexAI SfcI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 37 enzymes) #
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Asp700I 672 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 721 GGTGCC G^G[CT][GA]CC
BbsI 715 GTCTTC GAAGAC(2/6)
BbvCI 126 CCTCAGC CCTCAGC(-5/-2)
BglI 48 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 715 GTCTTC GAAGAC(2/6)
BsaAI 145 TACGTG [CT]AC^GT[GA]
BseRI 523 GAGGAG GAGGAG(10/8)
BsmAI 239 GTCTC GTCTC(1/5)
BspMI 595 ACCTGC ACCTGC(4/8)
BsrFI 768 GCCGGT [GA]^CCGG[CT]
BstAPI 856 GCAGGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 218 CCAGCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 768 GCCGGT [GA]^CCGG[CT]
DpnII 507 GATC ^GATC
DsaI 194 CCGCGG C^C[GA][CT]GG
EcoT14I 824 CCTTGG C^C[AT][AT]GG
KspI 194 CCGCGG CCGC^GG
MaeIII 419 GTGAC ^GT[ACGT]AC
MboI 507 GATC ^GATC
NdeII 507 GATC ^GATC
NotI 54 GCGGCCGC GC^GGCCGC
NspI 482 ACATGC [GA]CATG^[CT]
PshAI 29 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 194 CCGCGG CCGC^GG
SapI 168 GAAGAGC GCTCTTC(1/4)
Sau3AI 507 GATC ^GATC
SfiI 47 GGCCGGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 91 CCCGGG CCC^GGG
StyI 824 CCTTGG C^C[AT][AT]GG
TfiI 372 GATTC G^A[AT]TC
Tsp45I 419 GTGAC ^GT[GC]AC
Tsp509I 793 AATT ^AATT
XcmI 218 CCAGCGTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
XmnI 672 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 829 GAGCAC G[AT]GC[AT]^C
981 GTGCTC
AvaI 61 CCCGAG C^[CT]CG[GA]G
91 CCCGGG
AvaII 186 GGACC G^G[AT]CC
909 GGACC
BsiEI 46 CGGCCG CG[GA][CT]^CG
55 CGGCCG
BsiHKAI 829 GAGCAC G[AT]GC[AT]^C
981 GTGCTC
BsoBI 61 CCCGAG C^[CT]CG[GA]G
91 CCCGGG
BsrI 236 CCAGT ACTGG(1/-1)
696 CCAGT
DdeI 127 CTCAG C^T[ACGT]AG
966 CTCAG
DraII 87 GGGCCCC [GA]G^G[ACGT]CC[CT]
579 GGGCCCT
EaeI 46 CGGCCG [CT]^GGCC[GA]
55 CGGCCG
EagI 46 CGGCCG C^GGCCG
55 CGGCCG
EclXI 46 CGGCCG C^GGCCG
55 CGGCCG
Eco52I 46 CGGCCG C^GGCCG
55 CGGCCG
EcoO109I 87 GGGCCCC [GA]G^G[ACGT]CC[CT]
579 GGGCCCT
HgaI 209 GACGC GACGC(5/10)
221 GCGTC
MaeII 146 ACGT A^CGT
161 ACGT
SfaNI 393 GATGC GCATC(5/9)
281 GCATC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI ApaI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrBI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PvuII
Sau96I ScrFI TseI TspRI