Restriction map for
pFN21AE2621
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbsI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaI BseAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I ClaI DraI DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI MaeI MamI MfeI MflI
MroI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI RcaI SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 1226 GAATTC [GA]^AATT[CT]
AflIII 425 ACGCGT A^C[GA][CT]GT
AgeI 1359 ACCGGT A^CCGGT
AocI 1423 CCTGAGG CC^T[ACGT]AGG
ApoI 1226 GAATTC [GA]^AATT[CT]
BalI 1811 TGGCCA TGG^CCA
BbrPI 586 CACGTG CAC^GTG
BbvCI 1954 CCTCAGC CCTCAGC(-5/-2)
BcgI 1116 CGAAGTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1079 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1079 GCTCAGC GC^T[ACGT]AGC
BsaAI 586 CACGTG [CT]AC^GT[GA]
BsrBI 121 GAGCGG CCGCTC(-3/-3)
BsrDI 989 GCAATG GCAATG(2/0)
BssHII 476 GCGCGC G^CGCGC
BstXI 1214 CCATCGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1423 CCTGAGG CC^T[ACGT]AGG
CelII 1079 GCTCAGC GC^T[ACGT]AGC
CpoI 131 CGGTCCG CG^G[AT]CCG
Eco81I 1423 CCTGAGG CC^T[ACGT]AGG
EcoRI 1226 GAATTC G^AATTC
HindIII 701 AAGCTT A^AGCTT
KspI 657 CCGCGG CCGC^GG
MaeII 587 ACGT A^CGT
MluI 425 ACGCGT A^CGCGT
MluNI 1811 TGGCCA TGG^CCA
MscI 1811 TGGCCA TGG^CCA
MseI 1277 TTAA T^TAA
NaeI 511 GCCGGC GCC^GGC
NcoI 1744 CCATGG C^CATGG
NgoMIV 511 GCCGGC G^CCGGC
NspI 1596 GCATGC [GA]CATG^[CT]
PaeR7I 184 CTCGAG C^TCGAG
PinAI 1359 ACCGGT A^CCGGT
PmaCI 586 CACGTG CAC^GTG
PmlI 586 CACGTG CAC^GTG
PvuII 156 CAGCTG CAG^CTG
RsrII 131 CGGTCCG CG^G[AT]CCG
SacII 657 CCGCGG CCGC^GG
SapI 6 GCTCTTC GCTCTTC(1/4)
SexAI 677 ACCAGGT A^CC[AT]GGT
SmaI 720 CCCGGG CCC^GGG
SphI 1596 GCATGC GCATG^C
Tru9I 1277 TTAA T^TAA
XhoI 184 CTCGAG C^TCGAG
XmaCI 720 CCCGGG C^CCGGG
XmaI 720 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 881 GGTACC G^GTACC
1883 GGTACC
AlwI 17 GGATC GGATC(4/5)
547 GGATC
Asp718I 881 GGTACC G^GTACC
1883 GGTACC
BsaWI 129 TCCGGT [AT]^CCGG[AT]
1359 ACCGGT
BsiEI 513 CGGCCG CG[GA][CT]^CG
654 CGGCCG
BspMI 828 GCAGGT ACCTGC(4/8)
1553 GCAGGT
BsrFI 511 GCCGGC [GA]^CCGG[CT]
1359 ACCGGT
Cfr10I 511 GCCGGC [GA]^CCGG[CT]
1359 ACCGGT
DpnII 18 GATC ^GATC
548 GATC
DraIII 586 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
861 CACAGTGTG
DsaI 657 CCGCGG C^C[GA][CT]GG
1744 CCATGG
EagI 513 CGGCCG C^GGCCG
654 CGGCCG
EclXI 513 CGGCCG C^GGCCG
654 CGGCCG
Eco52I 513 CGGCCG C^GGCCG
654 CGGCCG
KpnI 881 GGTACC GGTAC^C
1883 GGTACC
MboI 18 GATC ^GATC
548 GATC
NdeII 18 GATC ^GATC
548 GATC
PflMI 909 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1053 CCAGTCGCTGG
SacI 103 GAGCTC GAGCT^C
1000 GAGCTC
Sau3AI 18 GATC ^GATC
548 GATC
SmlI 184 CTCGAG C^T[CT][GA]AG
1269 CTTGAG
TaqI 185 TCGA T^CGA
1690 TCGA
TfiI 1150 GAATC G^A[AT]TC
1183 GAATC
TthHB8I 185 TCGA T^CGA
1690 TCGA
Van91I 909 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1053 CCAGTCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau96I ScrFI SfaNI SfcI
SfoI StyI TseI Tsp45I Tsp509I
TspRI