Restriction map for
pFN21AE4410
##################################
# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BpmI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HapII Hin1I HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
ApaI 1147 GGGCCC GGGCC^C
Asp700I 676 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 814 GTGCTC G[AT]GC[AT]^C
AvaI 273 CTCGGG C^[CT]CG[GA]G
BbsI 1069 GTCTTC GAAGAC(2/6)
BbvCI 322 GCTGAGG CCTCAGC(-5/-2)
BglI 739 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 438 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 438 GCTCAGC GC^T[ACGT]AGC
BpuAI 1069 GTCTTC GAAGAC(2/6)
BsaI 706 GGTCTC GGTCTC(1/5)
BseRI 1132 GAGGAG GAGGAG(10/8)
BsiHKAI 814 GTGCTC G[AT]GC[AT]^C
BsmBI 924 CGTCTC CGTCTC(1/5)
BsmFI 177 GTCCC GGGAC(10/14)
BsmI 838 GCATTC GAATGC(1/-1)
BsoBI 273 CTCGGG C^[CT]CG[GA]G
BspMI 873 ACCTGC ACCTGC(4/8)
BstF5I 99 CATCC GGATG(2/0)
BstXI 1236 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 200 AGATCC [GA]^GATC[CT]
CelII 438 GCTCAGC GC^T[ACGT]AGC
DraI 639 TTTAAA TTT^AAA
EcoT14I 1236 CCAAGG C^C[AT][AT]GG
FokI 99 CATCC GGATG(9/13)
HgaI 923 GCGTC GACGC(5/10)
HincII 499 GTCAAC GT[CT]^[GA]AC
HindII 499 GTCAAC GT[CT]^[GA]AC
MflI 200 AGATCC [GA]^GATC[CT]
PstI 857 CTGCAG CTGCA^G
PvuII 16 CAGCTG CAG^CTG
SfaNI 213 GATGC GCATC(5/9)
SfcI 857 CTGCAG C^T[GA][CT]AG
SmlI 830 CTCAAG C^T[CT][GA]AG
StyI 1236 CCAAGG C^C[AT][AT]GG
TaqI 356 TCGA T^CGA
TthHB8I 356 TCGA T^CGA
XhoII 200 AGATCC [GA]^GATC[CT]
XmnI 676 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 373 AAATTC [GA]^AATT[CT]
824 AAATTT
Aor51HI 157 AGCGCT AGC^GCT
1136 AGCGCT
ApoI 373 AAATTC [GA]^AATT[CT]
824 AAATTT
BanII 31 GGGCTC G[GA]GC[CT]^C
1147 GGGCCC
BfaI 25 CTAG C^TAG
364 CTAG
BsaJI 863 CCCAGG C^C[ACGT][ACGT]GG
1236 CCAAGG
BsiYI 22 CCCCTAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
23 CCCTAGTGGGG
BslI 22 CCCCTAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
23 CCCTAGTGGGG
BsrDI 565 GCAATG GCAATG(2/0)
1262 GCAATG
Cac8I 282 GCTTGC GC[ACGT]^[ACGT]GC
744 GCAGGC
CfoI 158 GCGC GCG^C
1137 GCGC
EarI 915 CTCTTC CTCTTC(1/4)
597 GAAGAG
Eco47III 157 AGCGCT AGC^GCT
1136 AGCGCT
HaeII 157 AGCGCT [GA]GCGC^[CT]
1136 AGCGCT
HhaI 158 GCGC GCG^C
1137 GCGC
HinP1I 158 GCGC G^CGC
1137 GCGC
HphI 1040 TCACC GGTGA(8/7)
1157 TCACC
Ksp632I 915 CTCTTC CTCTTC(1/4)
597 GAAGAG
MaeI 25 CTAG C^TAG
364 CTAG
MaeII 449 ACGT A^CGT
1253 ACGT
MspA1I 16 CAGCTG C[AC]G^C[GT]G
953 CCGCTG
NlaIII 741 CATG CATG^
1142 CATG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BsmAI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI HaeIII HinfI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIV PleI
Sau3AI Sau96I ScrFI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI