Restriction map for
pFN21AE3872
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI AlwI
AocI ApaI ApoI AscI AseI
AsnI Asp718I AspEI AvaII AviII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BlpI Bpu1102I
BsaAI BsaBI BsaI BseRI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DsaI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI HphI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MscI MseI MslI MspA1I MunI
NcoI NdeI NheI NotI NruI
NsiI NspI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I TspRI
Van91I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 78 CGCG CG^CG
AcyI 446 GGCGCC G[GA]^CG[CT]C
AfaI 27 GTAC GT^AC
Alw44I 342 GTGCAC G^TGCAC
AlwNI 352 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 278 AGCGCT AGC^GCT
ApaLI 342 GTGCAC G^TGCAC
Asp700I 297 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 397 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 48 CCTAGG C^CTAGG
BbeI 446 GGCGCC GGCGC^C
BfaI 49 CTAG C^TAG
BglI 425 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 368 AGATCT A^GATCT
BlnI 48 CCTAGG C^CTAGG
BpmI 518 CTCCAG CTGGAG(16/14)
BsaHI 446 GGCGCC G[GA]^CG[CT]C
BsgI 349 GTGCAG GTGCAG(16/14)
BsmFI 337 GGGAC GGGAC(10/14)
BsrBI 62 CCGCTC CCGCTC(-3/-3)
BsrI 375 CCAGT ACTGG(1/-1)
BstBI 89 TTCGAA TT^CGAA
BstUI 78 CGCG CG^CG
BstYI 368 AGATCT [GA]^GATC[CT]
DraII 308 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 483 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 438 CGGCCG [CT]^GGCC[GA]
EagI 438 CGGCCG C^GGCCG
EarI 126 GAAGAG CTCTTC(1/4)
EclXI 438 CGGCCG C^GGCCG
Eco47III 278 AGCGCT AGC^GCT
Eco52I 438 CGGCCG C^GGCCG
EcoNI 459 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 308 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 48 CCTAGG C^C[AT][AT]GG
FauI 263 CCCGC CCCGC(4/6)
FseI 468 GGCCGGCC GGCCGG^CC
HgaI 79 GCGTC GACGC(5/10)
Hin1I 446 GGCGCC G[GA]^CG[CT]C
KasI 446 GGCGCC G^GCGCC
Ksp632I 126 GAAGAG CTCTTC(1/4)
MaeI 49 CTAG C^TAG
MflI 368 AGATCT [GA]^GATC[CT]
MvnI 78 CGCG CG^CG
NarI 446 GGCGCC GG^CGCC
NspV 89 TTCGAA TT^CGAA
PflFI 397 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 244 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 27 GTAC GT^AC
SfoI 446 GGCGCC GGC^GCC
SfuI 89 TTCGAA TT^CGAA
SmaI 474 CCCGGG CCC^GGG
StyI 48 CCTAGG C^C[AT][AT]GG
Tth111I 397 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 368 AGATCT [GA]^GATC[CT]
XmaCI 474 CCCGGG C^CCGGG
XmaI 474 CCCGGG C^CCGGG
XmnI 297 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 252 TCCGGA T^CCGGA
314 TCCGGA
AspHI 342 GTGCAC G[AT]GC[AT]^C
539 GTGCTC
BanI 223 GGTGCC G^G[CT][GA]CC
446 GGCGCC
BbsI 241 GAAGAC GAAGAC(2/6)
492 GTCTTC
BbvI 149 GCAGC GCAGC(8/12)
326 GCTGC
BpuAI 241 GAAGAC GAAGAC(2/6)
492 GTCTTC
BsaWI 252 TCCGGA [AT]^CCGG[AT]
314 TCCGGA
BseAI 252 TCCGGA T^CCGGA
314 TCCGGA
BsiEI 14 CGGTCG CG[GA][CT]^CG
438 CGGCCG
BsiHKAI 342 GTGCAC G[AT]GC[AT]^C
539 GTGCTC
BspEI 252 TCCGGA T^CCGGA
314 TCCGGA
DdeI 115 CTGAG C^T[ACGT]AG
497 CTAAG
DpnII 369 GATC ^GATC
420 GATC
MboI 369 GATC ^GATC
420 GATC
MroI 252 TCCGGA T^CCGGA
314 TCCGGA
NdeII 369 GATC ^GATC
420 GATC
NlaIII 216 CATG CATG^
294 CATG
Sau3AI 369 GATC ^GATC
420 GATC
SfaNI 256 GATGC GCATC(5/9)
266 GCATC
SgrAI 442 CGCCGGCG C[GA]^CCGG[CT]G
448 CGCCGGCG
TaqI 90 TCGA T^CGA
231 TCGA
TseI 149 GCAGC G^C[AT]GC
326 GCTGC
TthHB8I 90 TCGA T^CGA
231 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AvaI BanII BcnI
BmyI BsaJI BsiYI BslI BsoBI
Bsp1286I BsrFI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MboII MnlI MspI MvaI
MwoI NaeI NciI NgoMIV NlaIV
PleI Sau96I ScrFI