Restriction map for
pFN21AE6680
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspHI BspMI BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI HgaI HindIII HinfI
HpaI KpnI MaeI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PleI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SbfI
ScaI SfiI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 772 GTCTAC GT^[AC][GT]AC
ApaI 725 GGGCCC GGGCC^C
Asp700I 690 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 973 GTGCTC G[AT]GC[AT]^C
AvaI 976 CTCGAG C^[CT]CG[GA]G
AviII 146 TGCGCA TGC^GCA
BalI 884 TGGCCA TGG^CCA
BamHI 958 GGATCC G^GATCC
BbrPI 273 CACGTG CAC^GTG
BclI 204 TGATCA T^GATCA
BsaAI 273 CACGTG [CT]AC^GT[GA]
BsaBI 754 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 321 ACCGGA [AT]^CCGG[AT]
BseRI 58 GAGGAG GAGGAG(10/8)
BsgI 114 CTGCAC GTGCAG(16/14)
BsiHKAI 973 GTGCTC G[AT]GC[AT]^C
BsmAI 828 GAGAC GTCTC(1/5)
BsmFI 653 GGGAC GGGAC(10/14)
BsmI 841 GAATGC GAATGC(1/-1)
BsoBI 976 CTCGAG C^[CT]CG[GA]G
BspDI 379 ATCGAT AT^CGAT
BspLU11I 251 ACATGT A^CATGT
BsrDI 177 CATTGC GCAATG(2/0)
BsrI 892 ACTGG ACTGG(1/-1)
BssSI 283 CTCGTG CACGAG(-5/-1)
ClaI 379 ATCGAT AT^CGAT
DraII 724 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoNI 633 CCTTAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 724 GGGGCCC [GA]G^G[ACGT]CC[CT]
FbaI 204 TGATCA T^GATCA
FspI 146 TGCGCA TGC^GCA
KspI 75 CCGCGG CCGC^GG
MamI 754 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 884 TGGCCA TGG^CCA
MscI 884 TGGCCA TGG^CCA
NaeI 487 GCCGGC GCC^GGC
NgoMIV 487 GCCGGC G^CCGGC
NspI 251 ACATGT [GA]CATG^[CT]
PaeR7I 976 CTCGAG C^TCGAG
PmaCI 273 CACGTG CAC^GTG
PmlI 273 CACGTG CAC^GTG
PvuII 97 CAGCTG CAG^CTG
SacII 75 CCGCGG CCGC^GG
SapI 807 GAAGAGC GCTCTTC(1/4)
SgrAI 944 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 976 CTCGAG C^T[CT][GA]AG
Tsp509I 709 AATT ^AATT
XhoI 976 CTCGAG C^TCGAG
XmnI 690 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 76 CGCG CG^CG
162 CGCG
AflIII 251 ACATGT A^C[GA][CT]GT
274 ACGTGT
AvaII 522 GGTCC G^G[AT]CC
561 GGACC
BanI 12 GGCGCC G^G[CT][GA]CC
942 GGCGCC
BbeI 12 GGCGCC GGCGC^C
942 GGCGCC
BbvI 484 GCAGC GCAGC(8/12)
99 GCTGC
BcnI 564 CCCGG CC^[GC]GG
728 CCCGG
BsrFI 487 GCCGGC [GA]^CCGG[CT]
945 GCCGGT
BstUI 76 CGCG CG^CG
162 CGCG
BstYI 367 GGATCT [GA]^GATC[CT]
958 GGATCC
Cfr10I 487 GCCGGC [GA]^CCGG[CT]
945 GCCGGT
EaeI 566 CGGCCA [CT]^GGCC[GA]
884 TGGCCA
EarI 135 CTCTTC CTCTTC(1/4)
807 GAAGAG
HaeII 12 GGCGCC [GA]GCGC^[CT]
942 GGCGCC
KasI 12 GGCGCC G^GCGCC
942 GGCGCC
Ksp632I 135 CTCTTC CTCTTC(1/4)
807 GAAGAG
MaeIII 196 GTGAC ^GT[ACGT]AC
670 GTCAC
MflI 367 GGATCT [GA]^GATC[CT]
958 GGATCC
MspA1I 75 CCGCGG C[AC]G^C[GT]G
97 CAGCTG
MvnI 76 CGCG CG^CG
162 CGCG
NarI 12 GGCGCC GG^CGCC
942 GGCGCC
NciI 564 CCCGG CC^[GC]GG
728 CCCGG
SexAI 239 ACCTGGT A^CC[AT]GGT
476 ACCTGGT
SfcI 417 CTATAG C^T[GA][CT]AG
627 CTACAG
SfoI 12 GGCGCC GGC^GCC
942 GGCGCC
TseI 99 GCTGC G^C[AT]GC
484 GCAGC
Tsp45I 196 GTGAC ^GT[GC]AC
670 GTCAC
XhoII 367 GGATCT [GA]^GATC[CT]
958 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AatII AciI AcyI AfaI AluI
AlwI BanII BmyI BsaHI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DrdI DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HpaII HphI ItaI MaeII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TspRI TthHB8I