Restriction map for
pFN21AE4360
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BciVI
BfrI BlnI BpmI BsaAI BsaBI
BsaI BsiEI BsiWI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MscI MseI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 873 GACGTC GACGT^C
AccII 5 CGCG CG^CG
AccIII 995 TCCGGA T^CCGGA
AcsI 1000 AAATTC [GA]^AATT[CT]
AocI 1084 CCTGAGG CC^T[ACGT]AGG
ApoI 1000 AAATTC [GA]^AATT[CT]
AspHI 709 GAGCAC G[AT]GC[AT]^C
AvaI 365 CTCGAG C^[CT]CG[GA]G
BbsI 186 GAAGAC GAAGAC(2/6)
BbvCI 22 CCTCAGC CCTCAGC(-5/-2)
BcgI 1017 CGAAATGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 336 TGATCA T^GATCA
BfaI 823 CTAG C^TAG
BglI 768 GCCCCGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 506 AGATCT A^GATCT
BlpI 808 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 808 GCTGAGC GC^T[ACGT]AGC
BpuAI 186 GAAGAC GAAGAC(2/6)
BsaWI 995 TCCGGA [AT]^CCGG[AT]
BseAI 995 TCCGGA T^CCGGA
BsiHKAI 709 GAGCAC G[AT]GC[AT]^C
BsmBI 871 GAGACG CGTCTC(1/5)
BsoBI 365 CTCGAG C^[CT]CG[GA]G
BspEI 995 TCCGGA T^CCGGA
BspHI 833 TCATGA T^CATGA
BsrBI 973 CCGCTC CCGCTC(-3/-3)
BstAPI 355 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5 CGCG CG^CG
Bsu36I 1084 CCTGAGG CC^T[ACGT]AGG
CelII 808 GCTGAGC GC^T[ACGT]AGC
DsaI 890 CCATGG C^C[GA][CT]GG
Eco81I 1084 CCTGAGG CC^T[ACGT]AGG
EcoT22I 455 ATGCAT ATGCA^T
FbaI 336 TGATCA T^GATCA
MaeI 823 CTAG C^TAG
MroI 995 TCCGGA T^CCGGA
MvnI 5 CGCG CG^CG
NcoI 890 CCATGG C^CATGG
NsiI 455 ATGCAT ATGCA^T
NspI 777 GCATGT [GA]CATG^[CT]
PaeR7I 365 CTCGAG C^TCGAG
PflMI 890 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 949 CAGCTG CAG^CTG
RcaI 833 TCATGA T^CATGA
ScaI 720 AGTACT AGT^ACT
SmlI 365 CTCGAG C^T[CT][GA]AG
StuI 620 AGGCCT AGG^CCT
TaqI 366 TCGA T^CGA
TfiI 255 GAATC G^A[AT]TC
Tsp45I 469 GTCAC ^GT[GC]AC
Tsp509I 1001 AATT ^AATT
TthHB8I 366 TCGA T^CGA
Van91I 890 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 365 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BanI 484 GGTGCC G^G[CT][GA]CC
523 GGTGCC
BcnI 72 CCGGG CC^[GC]GG
441 CCGGG
BsgI 952 CTGCAC GTGCAG(16/14)
502 GTGCAG
BstYI 437 AGATCC [GA]^GATC[CT]
506 AGATCT
CfoI 6 GCGC GCG^C
981 GCGC
DraII 327 AGGACCC [GA]G^G[ACGT]CC[CT]
1040 GGGACCT
EcoO109I 327 AGGACCC [GA]G^G[ACGT]CC[CT]
1040 GGGACCT
EcoT14I 496 CCTTGG C^C[AT][AT]GG
890 CCATGG
FauI 105 CCCGC CCCGC(4/6)
197 CCCGC
HgaI 52 GACGC GACGC(5/10)
40 GCGTC
HhaI 6 GCGC GCG^C
981 GCGC
HinP1I 6 GCGC G^CGC
981 GCGC
MaeII 690 ACGT A^CGT
874 ACGT
MaeIII 409 GTAAC ^GT[ACGT]AC
469 GTCAC
MboII 186 GAAGA GAAGA(8/7)
837 GAAGA
MflI 437 AGATCC [GA]^GATC[CT]
506 AGATCT
MspA1I 106 CCGCTG C[AC]G^C[GT]G
949 CAGCTG
NciI 72 CCGGG CC^[GC]GG
441 CCGGG
PleI 371 GAGTC GAGTC(4/5)
630 GAGTC
PpuMI 327 AGGACCC [GA]G^G[AT]CC[CT]
1040 GGGACCT
SfaNI 313 GATGC GCATC(5/9)
454 GATGC
StyI 496 CCTTGG C^C[AT][AT]GG
890 CCATGG
XhoII 437 AGATCC [GA]^GATC[CT]
506 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaII BanII BbvI BmyI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII Hin1I HinfI HpaII HphI
ItaI MboI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfcI
TseI TspRI