Restriction map for
pFN21AE1753
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanII BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AcsI 670 AAATTC [GA]^AATT[CT]
AcyI 731 GGCGCC G[GA]^CG[CT]C
AfaI 18 GTAC GT^AC
AlwNI 742 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 168 CCTGAGG CC^T[ACGT]AGG
Aor51HI 451 AGCGCT AGC^GCT
ApoI 670 AAATTC [GA]^AATT[CT]
BamHI 173 GGATCC G^GATCC
BanI 731 GGCGCC G^G[CT][GA]CC
BbeI 731 GGCGCC GGCGC^C
BclI 153 TGATCA T^GATCA
BcnI 460 CCGGG CC^[GC]GG
BfaI 427 CTAG C^TAG
BlpI 739 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 739 GCTCAGC GC^T[ACGT]AGC
BsaHI 731 GGCGCC G[GA]^CG[CT]C
BseRI 599 CTCCTC GAGGAG(10/8)
BsgI 400 GTGCAG GTGCAG(16/14)
BspMI 660 GCAGGT ACCTGC(4/8)
BsrDI 63 CATTGC GCAATG(2/0)
BssSI 539 CTCGTG CACGAG(-5/-1)
BstF5I 252 GGATG GGATG(2/0)
BstYI 173 GGATCC [GA]^GATC[CT]
Bsu36I 168 CCTGAGG CC^T[ACGT]AGG
Cac8I 656 GCGAGC GC[ACGT]^[ACGT]GC
CelII 739 GCTCAGC GC^T[ACGT]AGC
DraII 243 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 419 TGGCCG [CT]^GGCC[GA]
EarI 602 CTCTTC CTCTTC(1/4)
Eco47III 451 AGCGCT AGC^GCT
Eco81I 168 CCTGAGG CC^T[ACGT]AGG
EcoO109I 243 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 68 CCAAGG C^C[AT][AT]GG
FbaI 153 TGATCA T^GATCA
FokI 252 GGATG GGATG(9/13)
HapII 460 CCGG C^CGG
HgaI 647 GCGTC GACGC(5/10)
Hin1I 731 GGCGCC G[GA]^CG[CT]C
HincII 91 GTTGAC GT[CT]^[GA]AC
HindII 91 GTTGAC GT[CT]^[GA]AC
HindIII 767 AAGCTT A^AGCTT
HpaII 460 CCGG C^CGG
HphI 700 GGTGA GGTGA(8/7)
KasI 731 GGCGCC G^GCGCC
Ksp632I 602 CTCTTC CTCTTC(1/4)
MaeI 427 CTAG C^TAG
MaeII 200 ACGT A^CGT
MaeIII 616 GTGAC ^GT[ACGT]AC
MflI 173 GGATCC [GA]^GATC[CT]
MspI 460 CCGG C^CGG
MwoI 647 GCGTCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 731 GGCGCC GG^CGCC
NciI 460 CCGGG CC^[GC]GG
NlaIII 190 CATG CATG^
PleI 214 GACTC GAGTC(4/5)
PpuMI 243 AGGACCC [GA]G^G[AT]CC[CT]
PstI 344 CTGCAG CTGCA^G
RsaI 18 GTAC GT^AC
SfcI 344 CTGCAG C^T[GA][CT]AG
SfoI 731 GGCGCC GGC^GCC
StyI 68 CCAAGG C^C[AT][AT]GG
Tsp45I 616 GTGAC ^GT[GC]AC
Tsp509I 671 AATT ^AATT
XhoII 173 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 6 enzymes) #
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BsmFI 3 GTCCC GGGAC(10/14)
84 GTCCC
MseI 497 TTAA T^TAA
683 TTAA
SmlI 235 CTCAAG C^T[CT][GA]AG
631 CTCAAG
TaqI 98 TCGA T^CGA
533 TCGA
Tru9I 497 TTAA T^TAA
683 TTAA
TthHB8I 98 TCGA T^CGA
533 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI AlwI AspHI AvaII BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstNI CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeII HaeIII HhaI HinP1I HinfI
ItaI MboI MboII MnlI MvaI
NdeII NlaIV Sau3AI Sau96I ScrFI
TfiI TseI TspRI