Restriction map for
pFN21AE6629
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 745 GAATTT [GA]^AATT[CT]
AfaI 336 GTAC GT^AC
AlwI 426 GATCC GGATC(4/5)
ApoI 745 GAATTT [GA]^AATT[CT]
Asp700I 742 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 844 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 40 CCCGAG C^[CT]CG[GA]G
BanII 415 GAGCTC G[GA]GC[CT]^C
BbvCI 756 GCTGAGG CCTCAGC(-5/-2)
BfaI 176 CTAG C^TAG
BglI 312 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI 410 CCATGG C^C[ACGT][ACGT]GG
BsiYI 39 CCCCGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 39 CCCCGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 40 CCCGAG C^[CT]CG[GA]G
BssKI 695 CCTGG ^CC[ACGT]GG
BstBI 749 TTCGAA TT^CGAA
BstNI 695 CCTGG CC^[AT]GG
DpnII 426 GATC ^GATC
DrdI 235 GACTGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 410 CCATGG C^C[GA][CT]GG
EcoRII 695 CCTGG ^CC[AT]GG
EcoT14I 410 CCATGG C^C[AT][AT]GG
FauI 636 GCGGG CCCGC(4/6)
HaeIII 824 GGCC GG^CC
HindIII 401 AAGCTT A^AGCTT
MaeI 176 CTAG C^TAG
MboI 426 GATC ^GATC
MvaI 695 CCTGG CC^[AT]GG
NcoI 410 CCATGG C^CATGG
NdeII 426 GATC ^GATC
NspV 749 TTCGAA TT^CGAA
PflFI 844 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 848 GAGTC GAGTC(4/5)
RsaI 336 GTAC GT^AC
SacI 415 GAGCTC GAGCT^C
Sau3AI 426 GATC ^GATC
ScrFI 695 CCTGG CC^[ACGT]GG
SfaNI 127 GATGC GCATC(5/9)
SfcI 733 CTACAG C^T[GA][CT]AG
SfuI 749 TTCGAA TT^CGAA
StyI 410 CCATGG C^C[AT][AT]GG
TfiI 485 GAATC G^A[AT]TC
Tth111I 844 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 742 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AflIII 525 ACATGT A^C[GA][CT]GT
765 ACATGT
AspHI 382 GAGCAC G[AT]GC[AT]^C
415 GAGCTC
AvaII 6 GGACC G^G[AT]CC
304 GGACC
BmyI 382 GAGCAC G[AGT]GC[ACT]^C
415 GAGCTC
BseRI 58 GAGGAG GAGGAG(10/8)
70 GAGGAG
BsiHKAI 382 GAGCAC G[AT]GC[AT]^C
415 GAGCTC
Bsp1286I 382 GAGCAC G[AGT]GC[ACT]^C
415 GAGCTC
BspLU11I 525 ACATGT A^CATGT
765 ACATGT
BsrDI 308 CATTGC GCAATG(2/0)
495 CATTGC
HinfI 485 GAATC G^A[ACGT]TC
848 GAGTC
NspI 525 ACATGT [GA]CATG^[CT]
765 ACATGT
SmlI 165 CTCAAG C^T[CT][GA]AG
314 CTCAAG
TaqI 20 TCGA T^CGA
750 TCGA
TthHB8I 20 TCGA T^CGA
750 TCGA
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BsmAI BsrI
BstF5I Cfr13I DdeI EarI Fnu4HI
FokI HphI ItaI Ksp632I MaeIII
MboII MnlI MseI MwoI NlaIII
Sau96I Tru9I TseI Tsp45I Tsp509I
TspRI