Restriction map for
pFN21AE3082
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BbvI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT22I FauI Fnu4HI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
ItaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MflI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
TseI Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 520 GTAGAC GT^[AC][GT]AC
AciI 150 CCGC CCGC(-3/-1)
AspHI 265 GAGCTC G[AT]GC[AT]^C
AvaII 710 GGACC G^G[AT]CC
BalI 716 TGGCCA TGG^CCA
BanI 142 GGTGCC G^G[CT][GA]CC
BcgI 135 CGATGGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 282 TGATCA T^GATCA
BsaJI 713 CCATGG C^C[ACGT][ACGT]GG
BseRI 703 CTCCTC GAGGAG(10/8)
BsiHKAI 265 GAGCTC G[AT]GC[AT]^C
BsmAI 209 GAGAC GTCTC(1/5)
BssKI 499 CCAGG ^CC[ACGT]GG
BstAPI 507 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 259 GGATG GGATG(2/0)
BstNI 499 CCAGG CC^[AT]GG
BstXI 700 CCACTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 710 GGACC G^G[ACGT]CC
DsaI 713 CCATGG C^C[GA][CT]GG
EaeI 716 TGGCCA [CT]^GGCC[GA]
EcoRI 196 GAATTC G^AATTC
EcoRII 499 CCAGG ^CC[AT]GG
EcoRV 160 GATATC GAT^ATC
EcoT14I 713 CCATGG C^C[AT][AT]GG
FbaI 282 TGATCA T^GATCA
FokI 259 GGATG GGATG(9/13)
HindIII 183 AAGCTT A^AGCTT
MluNI 716 TGGCCA TGG^CCA
MscI 716 TGGCCA TGG^CCA
MslI 525 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 499 CCAGG CC^[AT]GG
NcoI 713 CCATGG C^CATGG
PflMI 399 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 435 GACTC GAGTC(4/5)
SacI 265 GAGCTC GAGCT^C
Sau96I 710 GGACC G^G[ACGT]CC
ScrFI 499 CCAGG CC^[ACGT]GG
SfaNI 409 GCATC GCATC(5/9)
SmlI 598 CTCAAG C^T[CT][GA]AG
SphI 6 GCATGC GCATG^C
StyI 713 CCATGG C^C[AT][AT]GG
Van91I 399 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 45 GAATTT [GA]^AATT[CT]
196 GAATTC
AfaI 54 GTAC GT^AC
647 GTAC
ApoI 45 GAATTT [GA]^AATT[CT]
196 GAATTC
BanII 25 GAGCCC G[GA]GC[CT]^C
265 GAGCTC
BmyI 25 GAGCCC G[AGT]GC[ACT]^C
265 GAGCTC
BsiYI 399 CCAGATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
659 CCTACGACGGG
BslI 399 CCAGATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
659 CCTACGACGGG
Bsp1286I 25 GAGCCC G[AGT]GC[ACT]^C
265 GAGCTC
BspMI 488 ACCTGC ACCTGC(4/8)
579 GCAGGT
Cac8I 6 GCATGC GC[ACGT]^[ACGT]GC
624 GCTTGC
DdeI 254 CTAAG C^T[ACGT]AG
305 CTAAG
HphI 538 GGTGA GGTGA(8/7)
164 TCACC
MboII 330 GAAGA GAAGA(8/7)
337 GAAGA
MwoI 507 GCACAGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
570 GCCTTGTATGC
NspI 6 GCATGC [GA]CATG^[CT]
516 GCATGT
RsaI 54 GTAC GT^AC
647 GTAC
ScaI 53 AGTACT AGT^ACT
646 AGTACT
TfiI 217 GATTC G^A[AT]TC
475 GAATC
Tsp45I 367 GTGAC ^GT[GC]AC
679 GTGAC
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BsrI DpnII HaeIII HinfI
MaeIII MboI MnlI MseI NdeII
NlaIII NlaIV Sau3AI StuI Tru9I
Tsp509I TspRI