################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AflIII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcyI 652 GGCGTC G[GA]^CG[CT]C AflII 721 CTTAAG C^TTAAG Alw44I 1110 GTGCAC G^TGCAC AocI 111 CCTAAGG CC^T[ACGT]AGG ApaLI 1110 GTGCAC G^TGCAC Asp700I 982 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1110 GTGCAC G[AT]GC[AT]^C AvaII 766 GGACC G^G[AT]CC AvrII 1228 CCTAGG C^CTAGG BbrPI 1075 CACGTG CAC^GTG BclI 353 TGATCA T^GATCA BfaI 1229 CTAG C^TAG BfrI 721 CTTAAG C^TTAAG BlnI 1228 CCTAGG C^CTAGG BsaAI 1075 CACGTG [CT]AC^GT[GA] BsaBI 629 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 652 GGCGTC G[GA]^CG[CT]C BsiHKAI 1110 GTGCAC G[AT]GC[AT]^C BsiYI 624 CCTTCGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 624 CCTTCGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrDI 613 GCAATG GCAATG(2/0) BsrFI 619 ACCGGC [GA]^CCGG[CT] BstBI 23 TTCGAA TT^CGAA BstYI 634 GGATCT [GA]^GATC[CT] Bsu36I 111 CCTAAGG CC^T[ACGT]AGG Cfr10I 619 ACCGGC [GA]^CCGG[CT] DraI 13 TTTAAA TTT^AAA DraII 765 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 1086 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 1233 GAAGAG CTCTTC(1/4) Eco81I 111 CCTAAGG CC^T[ACGT]AGG EcoO109I 765 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 1228 CCTAGG C^C[AT][AT]GG FbaI 353 TGATCA T^GATCA Hin1I 652 GGCGTC G[GA]^CG[CT]C Ksp632I 1233 GAAGAG CTCTTC(1/4) MaeI 1229 CTAG C^TAG MaeII 1076 ACGT A^CGT MamI 629 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 634 GGATCT [GA]^GATC[CT] NlaIII 276 CATG CATG^ NlaIV 765 GGGACC GG[ACGT]^[ACGT]CC NspV 23 TTCGAA TT^CGAA PmaCI 1075 CACGTG CAC^GTG PmlI 1075 CACGTG CAC^GTG PpuMI 765 GGGACCT [GA]G^G[AT]CC[CT] ScaI 668 AGTACT AGT^ACT SfuI 23 TTCGAA TT^CGAA SmlI 721 CTTAAG C^T[CT][GA]AG SspI 834 AATATT AAT^ATT StuI 814 AGGCCT AGG^CCT StyI 1228 CCTAGG C^C[AT][AT]GG TfiI 438 GATTC G^A[AT]TC Tsp45I 283 GTGAC ^GT[GC]AC XhoII 634 GGATCT [GA]^GATC[CT] XmnI 982 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwI 1225 GATCC GGATC(4/5) 634 GGATC BcnI 897 CCCGG CC^[GC]GG 1125 CCCGG BsgI 1072 CTGCAC GTGCAG(16/14) 916 GTGCAG Bsp1407I 807 TGTACA T^GTACA 824 TGTACA BsrGI 807 TGTACA T^GTACA 824 TGTACA Cfr13I 682 GGGCC G^G[ACGT]CC 766 GGACC HgaI 653 GCGTC GACGC(5/10) 850 GCGTC MaeIII 283 GTGAC ^GT[ACGT]AC 394 GTTAC MslI 276 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 345 CACGGATGTG MspA1I 47 CAGCTG C[AC]G^C[GT]G 919 CAGCTG NciI 897 CCCGG CC^[GC]GG 1125 CCCGG PvuII 47 CAGCTG CAG^CTG 919 CAGCTG Sau96I 682 GGGCC G^G[ACGT]CC 766 GGACC SspBI 807 TGTACA T^GTACA 824 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AfaI AluI BanII BbvI BmyI BsaI BsaJI BseRI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MspI MvaI MwoI NdeII PleI PstI RsaI Sau3AI ScrFI SfaNI SfcI TaqI Tru9I TseI TspRI TthHB8I XcmI