Restriction map for
pFN21AE3184
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AflIII AgeI
AhdI AlwNI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII BalI BamHI
BanI BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI BpmI
Bpu1102I BpuAI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI ClaI CpoI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 652 GGCGTC G[GA]^CG[CT]C
AflII 721 CTTAAG C^TTAAG
Alw44I 1110 GTGCAC G^TGCAC
AocI 111 CCTAAGG CC^T[ACGT]AGG
ApaLI 1110 GTGCAC G^TGCAC
Asp700I 982 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1110 GTGCAC G[AT]GC[AT]^C
AvaII 766 GGACC G^G[AT]CC
AvrII 1228 CCTAGG C^CTAGG
BbrPI 1075 CACGTG CAC^GTG
BclI 353 TGATCA T^GATCA
BfaI 1229 CTAG C^TAG
BfrI 721 CTTAAG C^TTAAG
BlnI 1228 CCTAGG C^CTAGG
BsaAI 1075 CACGTG [CT]AC^GT[GA]
BsaBI 629 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 652 GGCGTC G[GA]^CG[CT]C
BsiHKAI 1110 GTGCAC G[AT]GC[AT]^C
BsiYI 624 CCTTCGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 624 CCTTCGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 613 GCAATG GCAATG(2/0)
BsrFI 619 ACCGGC [GA]^CCGG[CT]
BstBI 23 TTCGAA TT^CGAA
BstYI 634 GGATCT [GA]^GATC[CT]
Bsu36I 111 CCTAAGG CC^T[ACGT]AGG
Cfr10I 619 ACCGGC [GA]^CCGG[CT]
DraI 13 TTTAAA TTT^AAA
DraII 765 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1086 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1233 GAAGAG CTCTTC(1/4)
Eco81I 111 CCTAAGG CC^T[ACGT]AGG
EcoO109I 765 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1228 CCTAGG C^C[AT][AT]GG
FbaI 353 TGATCA T^GATCA
Hin1I 652 GGCGTC G[GA]^CG[CT]C
Ksp632I 1233 GAAGAG CTCTTC(1/4)
MaeI 1229 CTAG C^TAG
MaeII 1076 ACGT A^CGT
MamI 629 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 634 GGATCT [GA]^GATC[CT]
NlaIII 276 CATG CATG^
NlaIV 765 GGGACC GG[ACGT]^[ACGT]CC
NspV 23 TTCGAA TT^CGAA
PmaCI 1075 CACGTG CAC^GTG
PmlI 1075 CACGTG CAC^GTG
PpuMI 765 GGGACCT [GA]G^G[AT]CC[CT]
ScaI 668 AGTACT AGT^ACT
SfuI 23 TTCGAA TT^CGAA
SmlI 721 CTTAAG C^T[CT][GA]AG
SspI 834 AATATT AAT^ATT
StuI 814 AGGCCT AGG^CCT
StyI 1228 CCTAGG C^C[AT][AT]GG
TfiI 438 GATTC G^A[AT]TC
Tsp45I 283 GTGAC ^GT[GC]AC
XhoII 634 GGATCT [GA]^GATC[CT]
XmnI 982 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 1225 GATCC GGATC(4/5)
634 GGATC
BcnI 897 CCCGG CC^[GC]GG
1125 CCCGG
BsgI 1072 CTGCAC GTGCAG(16/14)
916 GTGCAG
Bsp1407I 807 TGTACA T^GTACA
824 TGTACA
BsrGI 807 TGTACA T^GTACA
824 TGTACA
Cfr13I 682 GGGCC G^G[ACGT]CC
766 GGACC
HgaI 653 GCGTC GACGC(5/10)
850 GCGTC
MaeIII 283 GTGAC ^GT[ACGT]AC
394 GTTAC
MslI 276 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
345 CACGGATGTG
MspA1I 47 CAGCTG C[AC]G^C[GT]G
919 CAGCTG
NciI 897 CCCGG CC^[GC]GG
1125 CCCGG
PvuII 47 CAGCTG CAG^CTG
919 CAGCTG
Sau96I 682 GGGCC G^G[ACGT]CC
766 GGACC
SspBI 807 TGTACA T^GTACA
824 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI BanII BbvI BmyI
BsaI BsaJI BseRI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
PleI PstI RsaI Sau3AI ScrFI
SfaNI SfcI TaqI Tru9I TseI
TspRI TthHB8I XcmI