Restriction map for
pFN21AB6288
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BciVI BfrI BglI BglII BlnI
BsaAI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI RsrII SacII SalI SapI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AclI 346 AACGTT AA^CGTT
AflIII 2172 ACATGT A^C[GA][CT]GT
AocI 110 CCTCAGG CC^T[ACGT]AGG
AvaI 2229 CTCGGG C^[CT]CG[GA]G
AvaII 690 GGTCC G^G[AT]CC
BanI 1549 GGTGCC G^G[CT][GA]CC
BbsI 1497 GTCTTC GAAGAC(2/6)
BbvCI 1234 CCTCAGC CCTCAGC(-5/-2)
BcgI 1352 CGACTCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 2127 CCCGG CC^[GC]GG
BlpI 1019 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1019 GCTCAGC GC^T[ACGT]AGC
BpuAI 1497 GTCTTC GAAGAC(2/6)
BsaWI 687 TCCGGT [AT]^CCGG[AT]
BsmAI 2085 GAGAC GTCTC(1/5)
BsoBI 2229 CTCGGG C^[CT]CG[GA]G
BspLU11I 2172 ACATGT A^CATGT
Bsu36I 110 CCTCAGG CC^T[ACGT]AGG
CelII 1019 GCTCAGC GC^T[ACGT]AGC
Cfr13I 690 GGTCC G^G[ACGT]CC
DraIII 2066 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 110 CCTCAGG CC^T[ACGT]AGG
EcoRI 2347 GAATTC G^AATTC
HincII 527 GTCAAC GT[CT]^[GA]AC
HindII 527 GTCAAC GT[CT]^[GA]AC
NciI 2127 CCCGG CC^[GC]GG
NspI 2172 ACATGT [GA]CATG^[CT]
Psp1406I 346 AACGTT AA^CGTT
PvuII 626 CAGCTG CAG^CTG
Sau96I 690 GGTCC G^G[ACGT]CC
ScaI 934 AGTACT AGT^ACT
SexAI 702 ACCTGGT A^CC[AT]GGT
XcmI 693 CCAAATTAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 641 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1025 CAGATACTG
BalI 849 TGGCCA TGG^CCA
1032 TGGCCA
BclI 1830 TGATCA T^GATCA
2157 TGATCA
BsaBI 1218 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2305 GATTCGAATC
BsgI 969 CTGCAC GTGCAG(16/14)
1642 GTGCAG
BsiYI 226 CCAAGTCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
827 CCGGAGGAGGG
BslI 226 CCAAGTCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
827 CCGGAGGAGGG
Bsp1407I 486 TGTACA T^GTACA
1413 TGTACA
BsrGI 486 TGTACA T^GTACA
1413 TGTACA
BstBI 1859 TTCGAA TT^CGAA
2307 TTCGAA
EaeI 849 TGGCCA [CT]^GGCC[GA]
1032 TGGCCA
FbaI 1830 TGATCA T^GATCA
2157 TGATCA
MamI 1218 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2305 GATTCGAATC
MluNI 849 TGGCCA TGG^CCA
1032 TGGCCA
MscI 849 TGGCCA TGG^CCA
1032 TGGCCA
NlaIII 2173 CATG CATG^
2340 CATG
NspV 1859 TTCGAA TT^CGAA
2307 TTCGAA
SfaNI 403 GCATC GCATC(5/9)
1582 GCATC
SfuI 1859 TTCGAA TT^CGAA
2307 TTCGAA
SmlI 752 CTCAAG C^T[CT][GA]AG
2300 CTCAAG
SpeI 550 ACTAGT A^CTAGT
1666 ACTAGT
SspBI 486 TGTACA T^GTACA
1413 TGTACA
SspI 652 AATATT AAT^ATT
1426 AATATT
TaqI 1860 TCGA T^CGA
2308 TCGA
TthHB8I 1860 TCGA T^CGA
2308 TCGA
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIV PleI PstI
RsaI SacI Sau3AI ScrFI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII