Restriction map for
pFN21AB7053
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbrPI BbsI BcgI BciVI BclI
BfrI BlnI BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MamI MluI MroI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 867 GTCTAC GT^[AC][GT]AC
AccII 6 CGCG CG^CG
AcsI 1622 AAATTT [GA]^AATT[CT]
Alw44I 1456 GTGCAC G^TGCAC
AocI 262 CCTCAGG CC^T[ACGT]AGG
Aor51HI 693 AGCGCT AGC^GCT
ApaI 158 GGGCCC GGGCC^C
ApaLI 1456 GTGCAC G^TGCAC
ApoI 1622 AAATTT [GA]^AATT[CT]
AvaII 906 GGACC G^G[AT]CC
BalI 1484 TGGCCA TGG^CCA
BamHI 61 GGATCC G^GATCC
BbeI 148 GGCGCC GGCGC^C
BfaI 481 CTAG C^TAG
BglII 44 AGATCT A^GATCT
BlpI 120 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 120 GCTGAGC GC^T[ACGT]AGC
BsaAI 1491 CACGTA [CT]AC^GT[GA]
BsaI 352 GAGACC GGTCTC(1/5)
BsgI 422 CTGCAC GTGCAG(16/14)
BsmAI 352 GAGAC GTCTC(1/5)
BsrBI 1050 CCGCTC CCGCTC(-3/-3)
BstUI 6 CGCG CG^CG
Bsu36I 262 CCTCAGG CC^T[ACGT]AGG
CelII 120 GCTGAGC GC^T[ACGT]AGC
DraIII 441 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 300 GACCGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 693 AGCGCT AGC^GCT
Eco81I 262 CCTCAGG CC^T[ACGT]AGG
HgaI 990 GACGC GACGC(5/10)
KasI 148 GGCGCC G^GCGCC
KspI 5 CCGCGG CCGC^GG
MaeI 481 CTAG C^TAG
MaeII 1492 ACGT A^CGT
MfeI 1616 CAATTG C^AATTG
MluNI 1484 TGGCCA TGG^CCA
MscI 1484 TGGCCA TGG^CCA
MseI 875 TTAA T^TAA
MslI 1113 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1616 CAATTG C^AATTG
MvnI 6 CGCG CG^CG
NarI 148 GGCGCC GG^CGCC
NcoI 83 CCATGG C^CATGG
PflMI 822 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 905 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 592 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 40 CTGCAG CTGCA^G
SacI 1060 GAGCTC GAGCT^C
SacII 5 CCGCGG CCGC^GG
SfcI 40 CTGCAG C^T[GA][CT]AG
SfiI 1293 GGCCTCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 148 GGCGCC GGC^GCC
SmaI 675 CCCGGG CCC^GGG
Tru9I 875 TTAA T^TAA
Tsp45I 1552 GTCAC ^GT[GC]AC
Van91I 822 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 675 CCCGGG C^CCGGG
XmaI 675 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 148 GGCGCC G[GA]^CG[CT]C
990 GACGCC
AlwNI 823 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
904 CAGGACCTG
Asp700I 813 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1095 GAAGCCCTTC
AvaI 451 CCCGAG C^[CT]CG[GA]G
675 CCCGGG
BbvCI 515 CCTCAGC CCTCAGC(-5/-2)
1544 CCTCAGC
BglI 151 GCCGCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1294 GCCTCTTCGGC
BsaHI 148 GGCGCC G[GA]^CG[CT]C
990 GACGCC
BsoBI 451 CCCGAG C^[CT]CG[GA]G
675 CCCGGG
BsrFI 501 ACCGGC [GA]^CCGG[CT]
977 ACCGGC
Cfr10I 501 ACCGGC [GA]^CCGG[CT]
977 ACCGGC
DsaI 5 CCGCGG C^C[GA][CT]GG
83 CCATGG
Hin1I 148 GGCGCC G[GA]^CG[CT]C
990 GACGCC
HinfI 307 GAGTC G^A[ACGT]TC
1027 GAGTC
NspI 959 GCATGC [GA]CATG^[CT]
1118 GCATGC
PleI 307 GAGTC GAGTC(4/5)
1027 GAGTC
PvuII 25 CAGCTG CAG^CTG
1547 CAGCTG
SphI 959 GCATGC GCATG^C
1118 GCATGC
StuI 668 AGGCCT AGG^CCT
878 AGGCCT
TaqI 378 TCGA T^CGA
1438 TCGA
Tsp509I 1617 AATT ^AATT
1623 AATT
TthHB8I 378 TCGA T^CGA
1438 TCGA
XmnI 813 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1095 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AviII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI XhoII