Restriction map for
pFN21AB6784
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# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT14I EcoT22I
FbaI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NarI NciI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tru9I Tsp45I TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 352 GCGG CCGC(-3/-1)
AcsI 360 GAATTC [GA]^AATT[CT]
AfaI 392 GTAC GT^AC
AluI 305 AGCT AG^CT
Alw44I 141 GTGCAC G^TGCAC
AlwI 309 GATCC GGATC(4/5)
ApaLI 141 GTGCAC G^TGCAC
ApoI 360 GAATTC [GA]^AATT[CT]
BbsI 132 GAAGAC GAAGAC(2/6)
BfaI 43 CTAG C^TAG
BglI 347 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 132 GAAGAC GAAGAC(2/6)
BsaI 4 GGTCTC GGTCTC(1/5)
BsgI 278 CTGCAC GTGCAG(16/14)
BsmAI 5 GTCTC GTCTC(1/5)
BspHI 428 TCATGA T^CATGA
BsrFI 52 GCCGGC [GA]^CCGG[CT]
Cfr10I 52 GCCGGC [GA]^CCGG[CT]
Cfr13I 345 GGGCC G^G[ACGT]CC
DdeI 191 CTGAG C^T[ACGT]AG
DraII 344 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 66 CCACGG C^C[GA][CT]GG
EcoNI 203 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 344 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 360 GAATTC G^AATTC
EcoRV 82 GATATC GAT^ATC
FauI 352 GCGGG CCCGC(4/6)
HaeIII 346 GGCC GG^CC
HincII 150 GTTGAC GT[CT]^[GA]AC
HindII 150 GTTGAC GT[CT]^[GA]AC
MaeI 43 CTAG C^TAG
MaeII 394 ACGT A^CGT
MaeIII 157 GTAAC ^GT[ACGT]AC
MspA1I 304 CAGCTG C[AC]G^C[GT]G
NaeI 52 GCCGGC GCC^GGC
NgoMIV 52 GCCGGC G^CCGGC
NheI 42 GCTAGC G^CTAGC
NspI 46 GCATGT [GA]CATG^[CT]
PleI 135 GACTC GAGTC(4/5)
PstI 207 CTGCAG CTGCA^G
PvuII 304 CAGCTG CAG^CTG
RcaI 428 TCATGA T^CATGA
RsaI 392 GTAC GT^AC
Sau96I 345 GGGCC G^G[ACGT]CC
SspI 440 AATATT AAT^ATT
TfiI 314 GATTC G^A[AT]TC
Tsp509I 361 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 141 GTGCAC G[AT]GC[AT]^C
223 GTGCTC
BmyI 141 GTGCAC G[AGT]GC[ACT]^C
223 GTGCTC
BsaJI 66 CCACGG C^C[ACGT][ACGT]GG
267 CCTGGG
BsiHKAI 141 GTGCAC G[AT]GC[AT]^C
223 GTGCTC
BsiYI 203 CCTTCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
246 CCATGAGAAGG
BslI 203 CCTTCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
246 CCATGAGAAGG
Bsp1286I 141 GTGCAC G[AGT]GC[ACT]^C
223 GTGCTC
BssKI 267 CCTGG ^CC[ACGT]GG
348 CCTGG
BstNI 267 CCTGG CC^[AT]GG
348 CCTGG
EcoRII 267 CCTGG ^CC[AT]GG
348 CCTGG
HapII 53 CCGG C^CGG
228 CCGG
HinfI 135 GACTC G^A[ACGT]TC
314 GATTC
HpaII 53 CCGG C^CGG
228 CCGG
HphI 8 TCACC GGTGA(8/7)
86 TCACC
MboII 132 GAAGA GAAGA(8/7)
35 TCTTC
MspI 53 CCGG C^CGG
228 CCGG
MvaI 267 CCTGG CC^[AT]GG
348 CCTGG
MwoI 297 GCACAAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
347 GCCTGGCGGGC
NlaIV 335 GGAACC GG[ACGT]^[ACGT]CC
344 GGGGCC
ScrFI 267 CCTGG CC^[ACGT]GG
348 CCTGG
SfcI 207 CTGCAG C^T[GA][CT]AG
237 CTACAG
TaqI 329 TCGA T^CGA
454 TCGA
TthHB8I 329 TCGA T^CGA
454 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BbvI Cac8I DpnII Fnu4HI ItaI
MboI MnlI NdeII NlaIII Sau3AI
TseI