##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmFI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MfeI        
MflI        MluI        MroI        MseI        MspA1I      
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AcyI          119  GGCGTC               G[GA]^CG[CT]C
AfaI          234  GTAC                 GT^AC
ApaI          273  GGGCCC               GGGCC^C
BalI          290  TGGCCA               TGG^CCA
BanII         273  GGGCCC               G[GA]GC[CT]^C
BglI          245  GCCTGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         119  GGCGTC               G[GA]^CG[CT]C
BsgI           50  CTGCAC               GTGCAG(16/14)
BsmBI         121  CGTCTC               CGTCTC(1/5)
BsmI           99  GAATGC               GAATGC(1/-1)
Bsp1407I      233  TGTACA               T^GTACA
BspMI         208  ACCTGC               ACCTGC(4/8)
BsrBI          60  CCGCTC               CCGCTC(-3/-3)
BsrGI         233  TGTACA               T^GTACA
BsrI          421  CCAGT                ACTGG(1/-1)
EaeI          290  TGGCCA               [CT]^GGCC[GA]
HgaI          120  GCGTC                GACGC(5/10)
Hin1I         119  GGCGTC               G[GA]^CG[CT]C
MluNI         290  TGGCCA               TGG^CCA
MscI          290  TGGCCA               TGG^CCA
MslI           94  CATGTGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI           93  GCATGT               [GA]CATG^[CT]
RsaI          234  GTAC                 GT^AC
SfaNI          44  GCATC                GCATC(5/9)
SmlI          268  CTCAAG               C^T[CT][GA]AG
SspBI         233  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AluI          230  AGCT                 AG^CT
              266  AGCT                
AlwNI          10  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              241  CAGGGCCTG           
BcnI          116  CCCGG                CC^[GC]GG
              324  CCCGG               
BmyI           27  GGGCAC               G[AGT]GC[ACT]^C
              273  GGGCCC              
BpmI          196  CTCCAG               CTGGAG(16/14)
              226  CTGGAG              
BsmAI         140  GAGAC                GTCTC(1/5)
              122  GTCTC               
Bsp1286I       27  GGGCAC               G[AGT]GC[ACT]^C
              273  GGGCCC              
BstAPI          9  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              240  GCAGGGCCTGC         
BstF5I        105  CATCC                GGATG(2/0)
              399  CATCC               
FokI          105  CATCC                GGATG(9/13)
              399  CATCC               
HapII         117  CCGG                 C^CGG
              325  CCGG                
HpaII         117  CCGG                 C^CGG
              325  CCGG                
HphI          260  TCACC                GGTGA(8/7)
              353  TCACC               
MspI          117  CCGG                 C^CGG
              325  CCGG                
NciI          116  CCCGG                CC^[GC]GG
              324  CCCGG               
NlaIII         94  CATG                 CATG^
              285  CATG                
PstI            7  CTGCAG               CTGCA^G
               39  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        BbvI        BsaJI       BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      ItaI        
MnlI        MvaI        MwoI        NlaIV       Sau96I      
ScrFI       SfcI        TseI        TspRI