Restriction map for
pFN21AB6778
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseRI BsgI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I CpoI DdeI DraI DraII
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
HaeII HgaI Hin1I HindIII KasI
KpnI Ksp632I KspI MaeI MamI
MflI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI TspRI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 377 TCCGGA T^CCGGA
AcsI 373 GAATTC [GA]^AATT[CT]
ApoI 373 GAATTC [GA]^AATT[CT]
Asp700I 369 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 39 GGTCC G^G[AT]CC
AviII 417 TGCGCA TGC^GCA
BalI 317 TGGCCA TGG^CCA
BbvI 141 GCAGC GCAGC(8/12)
BcnI 46 CCCGG CC^[GC]GG
BpmI 229 CTGGAG CTGGAG(16/14)
BsaWI 377 TCCGGA [AT]^CCGG[AT]
BseAI 377 TCCGGA T^CCGGA
BsiEI 323 CGGCCG CG[GA][CT]^CG
BspDI 115 ATCGAT AT^CGAT
BspEI 377 TCCGGA T^CCGGA
BsrI 365 ACTGG ACTGG(1/-1)
BstBI 215 TTCGAA TT^CGAA
BstF5I 340 CATCC GGATG(2/0)
CfoI 418 GCGC GCG^C
Cfr13I 39 GGTCC G^G[ACGT]CC
ClaI 115 ATCGAT AT^CGAT
DsaI 320 CCACGG C^C[GA][CT]GG
EagI 323 CGGCCG C^GGCCG
EclXI 323 CGGCCG C^GGCCG
Eco52I 323 CGGCCG C^GGCCG
EcoRI 373 GAATTC G^AATTC
FokI 340 CATCC GGATG(9/13)
FspI 417 TGCGCA TGC^GCA
HhaI 418 GCGC GCG^C
HinP1I 418 GCGC G^CGC
HincII 97 GTTAAC GT[CT]^[GA]AC
HindII 97 GTTAAC GT[CT]^[GA]AC
HinfI 50 GACTC G^A[ACGT]TC
HpaI 97 GTTAAC GTT^AAC
HphI 361 GGTGA GGTGA(8/7)
MaeII 391 ACGT A^CGT
MaeIII 362 GTGAC ^GT[ACGT]AC
MfeI 275 CAATTG C^AATTG
MluNI 317 TGGCCA TGG^CCA
MroI 377 TCCGGA T^CCGGA
MscI 317 TGGCCA TGG^CCA
MslI 195 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 275 CAATTG C^AATTG
NciI 46 CCCGG CC^[GC]GG
NruI 446 TCGCGA TCG^CGA
NspV 215 TTCGAA TT^CGAA
PflMI 30 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 50 GACTC GAGTC(4/5)
Sau96I 39 GGTCC G^G[ACGT]CC
SfaNI 268 GCATC GCATC(5/9)
SfuI 215 TTCGAA TT^CGAA
SmlI 409 CTTGAG C^T[CT][GA]AG
TseI 141 GCAGC G^C[AT]GC
Tsp45I 362 GTGAC ^GT[GC]AC
Van91I 30 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 369 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 335 CGCG CG^CG
447 CGCG
AluI 57 AGCT AG^CT
91 AGCT
BbsI 84 GAAGAC GAAGAC(2/6)
184 GAAGAC
BpuAI 84 GAAGAC GAAGAC(2/6)
184 GAAGAC
BstUI 335 CGCG CG^CG
447 CGCG
Cac8I 8 GCCTGC GC[ACGT]^[ACGT]GC
420 GCAGGC
DpnII 114 GATC ^GATC
450 GATC
EaeI 317 TGGCCA [CT]^GGCC[GA]
323 CGGCCG
HapII 47 CCGG C^CGG
378 CCGG
HpaII 47 CCGG C^CGG
378 CCGG
MboI 114 GATC ^GATC
450 GATC
MboII 84 GAAGA GAAGA(8/7)
184 GAAGA
MspI 47 CCGG C^CGG
378 CCGG
MvnI 335 CGCG CG^CG
447 CGCG
NdeII 114 GATC ^GATC
450 GATC
Sau3AI 114 GATC ^GATC
450 GATC
TaqI 116 TCGA T^CGA
216 TCGA
TthHB8I 116 TCGA T^CGA
216 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BsmI
BssKI BstNI EcoRII Fnu4HI HaeIII
ItaI MnlI MseI MvaI MwoI
NlaIII ScrFI Tru9I Tsp509I