Restriction map for
pFN21AE1935
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BciVI BclI BfrI
BglI BglII BlnI BsaBI BsaHI
BsaI BsiEI BsiWI BsmBI BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstUI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MamI MfeI MluI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 1035 GTATAC GT^[AC][GT]AC
AccIII 1068 TCCGGA T^CCGGA
AciI 226 GCGG CCGC(-3/-1)
Alw44I 930 GTGCAC G^TGCAC
AlwNI 317 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1291 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1331 AGCGCT AGC^GCT
ApaI 1164 GGGCCC GGGCC^C
ApaLI 930 GTGCAC G^TGCAC
AspHI 930 GTGCAC G[AT]GC[AT]^C
AvaI 961 CCCGAG C^[CT]CG[GA]G
BalI 821 TGGCCA TGG^CCA
BanII 1164 GGGCCC G[GA]GC[CT]^C
BbvCI 263 CCTCAGC CCTCAGC(-5/-2)
BcgI 355 CGAAAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 60 CCGGG CC^[GC]GG
BlpI 696 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 696 GCTCAGC GC^T[ACGT]AGC
BsaAI 406 TACGTG [CT]AC^GT[GA]
BsaWI 1068 TCCGGA [AT]^CCGG[AT]
BseAI 1068 TCCGGA T^CCGGA
BsiHKAI 930 GTGCAC G[AT]GC[AT]^C
BsmFI 1206 GGGAC GGGAC(10/14)
BsmI 1120 GAATGC GAATGC(1/-1)
BsoBI 961 CCCGAG C^[CT]CG[GA]G
Bsp1407I 140 TGTACA T^GTACA
BspEI 1068 TCCGGA T^CCGGA
BsrGI 140 TGTACA T^GTACA
Bst1107I 1035 GTATAC GTA^TAC
BstAPI 268 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 450 AGATCC [GA]^GATC[CT]
BstZ17I 1035 GTATAC GTA^TAC
Bsu36I 1291 CCTGAGG CC^T[ACGT]AGG
CelII 696 GCTCAGC GC^T[ACGT]AGC
CfoI 1332 GCGC GCG^C
EaeI 821 TGGCCA [CT]^GGCC[GA]
EarI 3 GAAGAG CTCTTC(1/4)
Eco47III 1331 AGCGCT AGC^GCT
Eco81I 1291 CCTGAGG CC^T[ACGT]AGG
EcoNI 1080 CCTCAGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1331 AGCGCT [GA]GCGC^[CT]
HhaI 1332 GCGC GCG^C
HinP1I 1332 GCGC G^CGC
HincII 1324 GTTAAC GT[CT]^[GA]AC
HindII 1324 GTTAAC GT[CT]^[GA]AC
HpaI 1324 GTTAAC GTT^AAC
Ksp632I 3 GAAGAG CTCTTC(1/4)
MflI 450 AGATCC [GA]^GATC[CT]
MluNI 821 TGGCCA TGG^CCA
MroI 1068 TCCGGA T^CCGGA
MscI 821 TGGCCA TGG^CCA
MspA1I 947 CAGCTG C[AC]G^C[GT]G
NciI 60 CCGGG CC^[GC]GG
NcoI 914 CCATGG C^CATGG
NspI 42 ACATGC [GA]CATG^[CT]
PshAI 892 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 947 CAGCTG CAG^CTG
SfaNI 1399 GATGC GCATC(5/9)
SmlI 1093 CTTGAG C^T[CT][GA]AG
SpeI 132 ACTAGT A^CTAGT
SspBI 140 TGTACA T^GTACA
SspI 485 AATATT AAT^ATT
TfiI 1387 GATTC G^A[AT]TC
XcmI 338 CCAAAGAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 450 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 451 GATCC GGATC(4/5)
1006 GATCC
BbsI 889 GAAGAC GAAGAC(2/6)
1135 GAAGAC
BfaI 133 CTAG C^TAG
760 CTAG
BmyI 930 GTGCAC G[AGT]GC[ACT]^C
1164 GGGCCC
BpmI 610 CTCCAG CTGGAG(16/14)
742 CTGGAG
BpuAI 889 GAAGAC GAAGAC(2/6)
1135 GAAGAC
BseRI 509 CTCCTC GAGGAG(10/8)
1373 CTCCTC
BsgI 333 CTGCAC GTGCAG(16/14)
1220 GTGCAG
BsiYI 384 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1080 CCTCAGTAAGG
BslI 384 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1080 CCTCAGTAAGG
BsmAI 606 GAGAC GTCTC(1/5)
202 GTCTC
Bsp1286I 930 GTGCAC G[AGT]GC[ACT]^C
1164 GGGCCC
BstF5I 780 CATCC GGATG(2/0)
747 GGATG
BstXI 1019 CCAAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1304 CCACGGAACTGG
DsaI 914 CCATGG C^C[GA][CT]GG
1304 CCACGG
EcoT14I 914 CCATGG C^C[AT][AT]GG
1113 CCTTGG
FokI 780 CATCC GGATG(9/13)
747 GGATG
HapII 60 CCGG C^CGG
1069 CCGG
HpaII 60 CCGG C^CGG
1069 CCGG
MaeI 133 CTAG C^TAG
760 CTAG
MspI 60 CCGG C^CGG
1069 CCGG
StyI 914 CCATGG C^C[AT][AT]GG
1113 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvI BsaJI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
Tru9I TseI Tsp45I Tsp509I TspRI