Restriction map for
pFN21AE5282
##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvnI NarI
NciI NcoI NdeI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AspHI 383 GTGCTC G[AT]GC[AT]^C
BanII 199 GGGCTC G[GA]GC[CT]^C
BbsI 175 GTCTTC GAAGAC(2/6)
BglI 154 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 175 GTCTTC GAAGAC(2/6)
BsaI 518 GAGACC GGTCTC(1/5)
BsiHKAI 383 GTGCTC G[AT]GC[AT]^C
BsmAI 518 GAGAC GTCTC(1/5)
BspDI 749 ATCGAT AT^CGAT
BsrFI 159 GCCGGC [GA]^CCGG[CT]
BstAPI 661 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 54 GGTCACC G^GT[ACGT]ACC
BstPI 54 GGTCACC G^GT[ACGT]ACC
CfoI 26 GCGC GCG^C
Cfr10I 159 GCCGGC [GA]^CCGG[CT]
ClaI 749 ATCGAT AT^CGAT
DdeI 205 CTGAG C^T[ACGT]AG
EaeI 69 TGGCCG [CT]^GGCC[GA]
EcoO65I 54 GGTCACC G^GT[ACGT]ACC
HaeII 25 AGCGCC [GA]GCGC^[CT]
HapII 160 CCGG C^CGG
HhaI 26 GCGC GCG^C
HinP1I 26 GCGC G^CGC
HindIII 413 AAGCTT A^AGCTT
HpaII 160 CCGG C^CGG
MspI 160 CCGG C^CGG
NaeI 159 GCCGGC GCC^GGC
NgoMIV 159 GCCGGC G^CCGGC
NheI 733 GCTAGC G^CTAGC
NlaIII 138 CATG CATG^
PleI 481 GAGTC GAGTC(4/5)
SspI 718 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 230 AAATTC [GA]^AATT[CT]
571 AAATTC
ApoI 230 AAATTC [GA]^AATT[CT]
571 AAATTC
BbvI 109 GCAGC GCAGC(8/12)
397 GCAGC
BfaI 478 CTAG C^TAG
734 CTAG
BmyI 199 GGGCTC G[AGT]GC[ACT]^C
383 GTGCTC
BseRI 37 GAGGAG GAGGAG(10/8)
148 GAGGAG
Bsp1286I 199 GGGCTC G[AGT]GC[ACT]^C
383 GTGCTC
BsrI 272 ACTGG ACTGG(1/-1)
235 CCAGT
Cfr13I 132 GGGCC G^G[ACGT]CC
170 GGCCC
DpnII 740 GATC ^GATC
748 GATC
EarI 472 CTCTTC CTCTTC(1/4)
165 GAAGAG
HphI 56 TCACC GGTGA(8/7)
575 TCACC
Ksp632I 472 CTCTTC CTCTTC(1/4)
165 GAAGAG
MaeI 478 CTAG C^TAG
734 CTAG
MaeIII 55 GTCAC ^GT[ACGT]AC
589 GTGAC
MboI 740 GATC ^GATC
748 GATC
MseI 326 TTAA T^TAA
503 TTAA
MspA1I 100 CAGCTG C[AC]G^C[GT]G
398 CAGCTG
NdeII 740 GATC ^GATC
748 GATC
NlaIV 200 GGCTCC GG[ACGT]^[ACGT]CC
637 GGCTCC
PvuII 100 CAGCTG CAG^CTG
398 CAGCTG
Sau3AI 740 GATC ^GATC
748 GATC
Sau96I 132 GGGCC G^G[ACGT]CC
170 GGCCC
SfaNI 127 GATGC GCATC(5/9)
442 GATGC
TaqI 723 TCGA T^CGA
750 TCGA
Tru9I 326 TTAA T^TAA
503 TTAA
TseI 109 GCAGC G^C[AT]GC
397 GCAGC
Tsp45I 55 GTCAC ^GT[GC]AC
589 GTGAC
TthHB8I 723 TCGA T^CGA
750 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BsmFI BssKI BstF5I BstNI Cac8I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HinfI ItaI MboII MnlI
MvaI MwoI ScrFI StyI TfiI
Tsp509I