################################## # NO CUT ENZYMES ( 222 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TseI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AcsI 176 GAATTT [GA]^AATT[CT] AfaI 32 GTAC GT^AC Alw44I 193 GTGCAC G^TGCAC AlwI 142 GATCC GGATC(4/5) ApaLI 193 GTGCAC G^TGCAC ApoI 176 GAATTT [GA]^AATT[CT] Asp700I 156 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 193 GTGCAC G[AT]GC[AT]^C BciVI 273 GGATAC GTATCC(6/5) BfaI 68 CTAG C^TAG BmyI 193 GTGCAC G[AGT]GC[ACT]^C BpmI 44 CTCCAG CTGGAG(16/14) BsaJI 219 CCAGGG C^C[ACGT][ACGT]GG BsiHKAI 193 GTGCAC G[AT]GC[AT]^C Bsp1286I 193 GTGCAC G[AGT]GC[ACT]^C BstXI 46 CCAGATACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 142 GATC ^GATC HindIII 113 AAGCTT A^AGCTT MaeI 68 CTAG C^TAG MboI 142 GATC ^GATC MboII 92 GAAGA GAAGA(8/7) NdeI 146 CATATG CA^TATG NdeII 142 GATC ^GATC NlaIII 53 CATG CATG^ RsaI 32 GTAC GT^AC Sau3AI 142 GATC ^GATC SexAI 201 ACCAGGT A^CC[AT]GGT SfaNI 242 GCATC GCATC(5/9) Tsp509I 177 AATT ^AATT XmnI 156 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### AluI 98 AGCT AG^CT 114 AGCT BssKI 202 CCAGG ^CC[ACGT]GG 219 CCAGG BstNI 202 CCAGG CC^[AT]GG 219 CCAGG EcoRII 202 CCAGG ^CC[AT]GG 219 CCAGG MvaI 202 CCAGG CC^[AT]GG 219 CCAGG ScrFI 202 CCAGG CC^[ACGT]GG 219 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 0 enzymes) # #########################################