##################################
# NO CUT ENZYMES (  222 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DdeI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaI        
HpaII       HphI        ItaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MnlI        
MroI        MscI        MseI        MslI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AcsI          176  GAATTT               [GA]^AATT[CT]
AfaI           32  GTAC                 GT^AC
Alw44I        193  GTGCAC               G^TGCAC
AlwI          142  GATCC                GGATC(4/5)
ApaLI         193  GTGCAC               G^TGCAC
ApoI          176  GAATTT               [GA]^AATT[CT]
Asp700I       156  GAATAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         193  GTGCAC               G[AT]GC[AT]^C
BciVI         273  GGATAC               GTATCC(6/5)
BfaI           68  CTAG                 C^TAG
BmyI          193  GTGCAC               G[AGT]GC[ACT]^C
BpmI           44  CTCCAG               CTGGAG(16/14)
BsaJI         219  CCAGGG               C^C[ACGT][ACGT]GG
BsiHKAI       193  GTGCAC               G[AT]GC[AT]^C
Bsp1286I      193  GTGCAC               G[AGT]GC[ACT]^C
BstXI          46  CCAGATACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         142  GATC                 ^GATC
HindIII       113  AAGCTT               A^AGCTT
MaeI           68  CTAG                 C^TAG
MboI          142  GATC                 ^GATC
MboII          92  GAAGA                GAAGA(8/7)
NdeI          146  CATATG               CA^TATG
NdeII         142  GATC                 ^GATC
NlaIII         53  CATG                 CATG^
RsaI           32  GTAC                 GT^AC
Sau3AI        142  GATC                 ^GATC
SexAI         201  ACCAGGT              A^CC[AT]GGT
SfaNI         242  GCATC                GCATC(5/9)
Tsp509I       177  AATT                 ^AATT
XmnI          156  GAATAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AluI           98  AGCT                 AG^CT
              114  AGCT                
BssKI         202  CCAGG                ^CC[ACGT]GG
              219  CCAGG               
BstNI         202  CCAGG                CC^[AT]GG
              219  CCAGG               
EcoRII        202  CCAGG                ^CC[AT]GG
              219  CCAGG               
MvaI          202  CCAGG                CC^[AT]GG
              219  CCAGG               
ScrFI         202  CCAGG                CC^[ACGT]GG
              219  CCAGG               


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################