##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       AccI        AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
AspEI       AspI        AviII       AvrII       BamHI       
BbsI        BcgI        BciVI       BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspHI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     CelII       CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HincII      HindII      
HpaI        KspI        MamI        MfeI        MluI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
TfiI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

Acc65I       1283  GGTACC               G^GTACC
AccIII       1082  TCCGGA               T^CCGGA
AclI         1213  AACGTT               AA^CGTT
AcsI         1179  AAATTT               [GA]^AATT[CT]
AflII         478  CTTAAG               C^TTAAG
AlwNI         672  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          541  CCTGAGG              CC^T[ACGT]AGG
ApoI         1179  AAATTT               [GA]^AATT[CT]
AseI          766  ATTAAT               AT^TAAT
AsnI          766  ATTAAT               AT^TAAT
Asp718I      1283  GGTACC               G^GTACC
AspHI         691  GAGCAC               G[AT]GC[AT]^C
BalI          232  TGGCCA               TGG^CCA
BbeI          112  GGCGCC               GGCGC^C
BbvCI        1590  GCTGAGG              CCTCAGC(-5/-2)
BclI          422  TGATCA               T^GATCA
BfrI          478  CTTAAG               C^TTAAG
BglI          442  GCCAGGTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1010  AGATCT               A^GATCT
BsaI          205  GAGACC               GGTCTC(1/5)
BsaWI        1082  TCCGGA               [AT]^CCGG[AT]
BseAI        1082  TCCGGA               T^CCGGA
BseRI         995  GAGGAG               GAGGAG(10/8)
BsgI         1059  GTGCAG               GTGCAG(16/14)
BsiHKAI       691  GAGCAC               G[AT]GC[AT]^C
BsmAI         205  GAGAC                GTCTC(1/5)
BspDI         757  ATCGAT               AT^CGAT
BspEI        1082  TCCGGA               T^CCGGA
BspLU11I       65  ACATGT               A^CATGT
BspMI        1703  ACCTGC               ACCTGC(4/8)
BstAPI       1607  GCAACCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         335  TTCGAA               TT^CGAA
BstXI         410  CCACCAGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        541  CCTGAGG              CC^T[ACGT]AGG
ClaI          757  ATCGAT               AT^CGAT
DsaI          464  CCGTGG               C^C[GA][CT]GG
EarI          184  GAAGAG               CTCTTC(1/4)
Eco81I        541  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       650  CCAAGG               C^C[AT][AT]GG
FbaI          422  TGATCA               T^GATCA
HgaI          855  GACGC                GACGC(5/10)
HinfI         735  GACTC                G^A[ACGT]TC
KasI          112  GGCGCC               G^GCGCC
KpnI         1283  GGTACC               GGTAC^C
Ksp632I       184  GAAGAG               CTCTTC(1/4)
MluNI         232  TGGCCA               TGG^CCA
MroI         1082  TCCGGA               T^CCGGA
MscI          232  TGGCCA               TGG^CCA
NarI          112  GGCGCC               GG^CGCC
NspI           65  ACATGT               [GA]CATG^[CT]
NspV          335  TTCGAA               TT^CGAA
PleI          735  GACTC                GAGTC(4/5)
PshBI         766  ATTAAT               AT^TAAT
Psp1406I     1213  AACGTT               AA^CGTT
PvuII        1263  CAGCTG               CAG^CTG
SexAI         314  ACCAGGT              A^CC[AT]GGT
SfoI          112  GGCGCC               GGC^GCC
SfuI          335  TTCGAA               TT^CGAA
SmaI         1107  CCCGGG               CCC^GGG
SmlI          478  CTTAAG               C^T[CT][GA]AG
SspI         1228  AATATT               AAT^ATT
StuI          606  AGGCCT               AGG^CCT
StyI          650  CCAAGG               C^C[AT][AT]GG
XmaCI        1107  CCCGGG               C^CCGGG
XmaI         1107  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          112  GGCGCC               G[GA]^CG[CT]C
              855  GACGCC              
AlwI          538  GATCC                GGATC(4/5)
              399  GGATC               
BbrPI        1075  CACGTG               CAC^GTG
             1597  CACGTG              
BfaI         1006  CTAG                 C^TAG
             1208  CTAG                
BsaAI        1075  CACGTG               [CT]AC^GT[GA]
             1597  CACGTG              
BsaHI         112  GGCGCC               G[GA]^CG[CT]C
              855  GACGCC              
BsrBI         276  CCGCTC               CCGCTC(-3/-3)
             1638  GAGCGG              
BsrDI         176  GCAATG               GCAATG(2/0)
              533  GCAATG              
BsrFI         115  GCCGGC               [GA]^CCGG[CT]
              405  GCCGGC              
BstYI         399  GGATCT               [GA]^GATC[CT]
             1010  AGATCT              
Cfr10I        115  GCCGGC               [GA]^CCGG[CT]
              405  GCCGGC              
HaeII           8  GGCGCT               [GA]GCGC^[CT]
              112  GGCGCC              
Hin1I         112  GGCGCC               G[GA]^CG[CT]C
              855  GACGCC              
HindIII       251  AAGCTT               A^AGCTT
              331  AAGCTT              
MaeI         1006  CTAG                 C^TAG
             1208  CTAG                
MflI          399  GGATCT               [GA]^GATC[CT]
             1010  AGATCT              
MspA1I        379  CAGCGG               C[AC]G^C[GT]G
             1263  CAGCTG              
NaeI          115  GCCGGC               GCC^GGC
              405  GCCGGC              
NgoMIV        115  GCCGGC               G^CCGGC
              405  GCCGGC              
PmaCI        1075  CACGTG               CAC^GTG
             1597  CACGTG              
PmlI         1075  CACGTG               CAC^GTG
             1597  CACGTG              
PstI         1528  CTGCAG               CTGCA^G
             1705  CTGCAG              
XhoII         399  GGATCT               [GA]^GATC[CT]
             1010  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PpuMI       PshAI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I