Restriction map for
pFN21AE6575
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HinfI HpaI HphI
KpnI MaeI MaeIII MamI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tsp45I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 562 GAATTC [GA]^AATT[CT]
AcyI 570 GGCGCC G[GA]^CG[CT]C
AgeI 166 ACCGGT A^CCGGT
AlwI 261 GATCC GGATC(4/5)
AlwNI 304 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 291 CCTCAGG CC^T[ACGT]AGG
ApoI 562 GAATTC [GA]^AATT[CT]
AvaI 325 CCCGAG C^[CT]CG[GA]G
BanI 570 GGCGCC G^G[CT][GA]CC
BbeI 570 GGCGCC GGCGC^C
BbvCI 216 CCTCAGC CCTCAGC(-5/-2)
BsaHI 570 GGCGCC G[GA]^CG[CT]C
BsaWI 166 ACCGGT [AT]^CCGG[AT]
BseRI 397 GAGGAG GAGGAG(10/8)
BsmBI 79 GAGACG CGTCTC(1/5)
BsoBI 325 CCCGAG C^[CT]CG[GA]G
BsrDI 143 GCAATG GCAATG(2/0)
BsrFI 166 ACCGGT [GA]^CCGG[CT]
BsrI 420 CCAGT ACTGG(1/-1)
BstYI 260 AGATCC [GA]^GATC[CT]
Bsu36I 291 CCTCAGG CC^T[ACGT]AGG
Cfr10I 166 ACCGGT [GA]^CCGG[CT]
DpnII 261 GATC ^GATC
DsaI 438 CCGCGG C^C[GA][CT]GG
Eco81I 291 CCTCAGG CC^T[ACGT]AGG
EcoRI 562 GAATTC G^AATTC
HaeII 570 GGCGCC [GA]GCGC^[CT]
Hin1I 570 GGCGCC G[GA]^CG[CT]C
KasI 570 GGCGCC G^GCGCC
KspI 438 CCGCGG CCGC^GG
MaeII 515 ACGT A^CGT
MboI 261 GATC ^GATC
MflI 260 AGATCC [GA]^GATC[CT]
MseI 257 TTAA T^TAA
MslI 165 CACCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 570 GGCGCC GG^CGCC
NdeII 261 GATC ^GATC
NspI 554 GCATGC [GA]CATG^[CT]
PinAI 166 ACCGGT A^CCGGT
PpuMI 371 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 364 CTGCAG CTGCA^G
SacII 438 CCGCGG CCGC^GG
SapI 348 GCTCTTC GCTCTTC(1/4)
Sau3AI 261 GATC ^GATC
SfoI 570 GGCGCC GGC^GCC
SgrAI 165 CACCGGTG C[GA]^CCGG[CT]G
SphI 554 GCATGC GCATG^C
Tru9I 257 TTAA T^TAA
Tsp509I 563 AATT ^AATT
XcmI 538 CCACTGCGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 260 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 188 TGGCCA TGG^CCA
449 TGGCCA
BanII 57 GAGCCC G[GA]GC[CT]^C
322 GAGCCC
BmyI 57 GAGCCC G[AGT]GC[ACT]^C
322 GAGCCC
BsgI 162 CTGCAC GTGCAG(16/14)
331 GTGCAG
BsmAI 79 GAGAC GTCTC(1/5)
3 GTCTC
BsmFI 524 GGGAC GGGAC(10/14)
342 GTCCC
Bsp1286I 57 GAGCCC G[AGT]GC[ACT]^C
322 GAGCCC
BssHII 467 GCGCGC G^CGCGC
469 GCGCGC
DraII 32 AGGCCCC [GA]G^G[ACGT]CC[CT]
371 AGGTCCT
EaeI 188 TGGCCA [CT]^GGCC[GA]
449 TGGCCA
EarI 349 CTCTTC CTCTTC(1/4)
394 GAAGAG
EcoO109I 32 AGGCCCC [GA]G^G[ACGT]CC[CT]
371 AGGTCCT
HgaI 81 GACGC GACGC(5/10)
414 GACGC
Ksp632I 349 CTCTTC CTCTTC(1/4)
394 GAAGAG
MluNI 188 TGGCCA TGG^CCA
449 TGGCCA
MscI 188 TGGCCA TGG^CCA
449 TGGCCA
NlaIII 14 CATG CATG^
555 CATG
SfaNI 108 GATGC GCATC(5/9)
473 GCATC
SfcI 177 CTACAG C^T[GA][CT]AG
364 CTGCAG
TspRI 421 CAGTG CA[GC]TG[ACGT][ACGT]^
539 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BpmI BsaJI BsiYI BslI
BsrBI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV PvuII Sau96I
ScrFI TseI