Restriction map for
pFN21AE5181
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# NO CUT ENZYMES ( 202 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI ItaI KasI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MflI MluI MluNI MroI
MscI MseI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I TseI
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AluI 324 AGCT AG^CT
AlwNI 112 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 235 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 89 GGACC G^G[AT]CC
BsaJI 24 CCTTGG C^C[ACGT][ACGT]GG
BsmBI 233 GAGACG CGTCTC(1/5)
BsrFI 120 GCCGGT [GA]^CCGG[CT]
BsrI 337 ACTGG ACTGG(1/-1)
BssKI 15 CCTGG ^CC[ACGT]GG
BstF5I 12 CATCC GGATG(2/0)
BstNI 15 CCTGG CC^[AT]GG
Cfr10I 120 GCCGGT [GA]^CCGG[CT]
DdeI 78 CTCAG C^T[ACGT]AG
DraII 88 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 88 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 15 CCTGG ^CC[AT]GG
EcoT14I 24 CCTTGG C^C[AT][AT]GG
FokI 12 CATCC GGATG(9/13)
HapII 121 CCGG C^CGG
HpaII 121 CCGG C^CGG
HphI 228 GGTGA GGTGA(8/7)
MaeIII 328 GTGAC ^GT[ACGT]AC
MslI 222 CACATGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 323 CAGCTG C[AC]G^C[GT]G
MspI 121 CCGG C^CGG
MvaI 15 CCTGG CC^[AT]GG
PflFI 235 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 330 GACTC GAGTC(4/5)
PpuMI 88 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 323 CAGCTG CAG^CTG
ScrFI 15 CCTGG CC^[ACGT]GG
SfaNI 29 GCATC GCATC(5/9)
SmlI 162 CTTGAG C^T[CT][GA]AG
StyI 24 CCTTGG C^C[AT][AT]GG
TaqI 313 TCGA T^CGA
TfiI 131 GAATC G^A[AT]TC
Tsp45I 328 GTGAC ^GT[GC]AC
Tsp509I 174 AATT ^AATT
TspRI 42 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 235 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 313 TCGA T^CGA
XcmI 214 CCACAAGCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AlwI 211 GATCC GGATC(4/5)
282 GATCC
Cfr13I 18 GGCCC G^G[ACGT]CC
89 GGACC
DpnII 211 GATC ^GATC
282 GATC
HaeIII 18 GGCC GG^CC
193 GGCC
HinfI 131 GAATC G^A[ACGT]TC
330 GACTC
MaeII 189 ACGT A^CGT
236 ACGT
MboI 211 GATC ^GATC
282 GATC
NdeII 211 GATC ^GATC
282 GATC
NlaIV 157 GGCTCC GG[ACGT]^[ACGT]CC
301 GGAACC
Sau3AI 211 GATC ^GATC
282 GATC
Sau96I 18 GGCCC G^G[ACGT]CC
89 GGACC
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
BsmAI MnlI NlaIII