################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FokI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MflI MluI MluNI MroI MscI MslI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 675 ACGTGT A^C[GA][CT]GT Aor51HI 452 AGCGCT AGC^GCT BbrPI 674 CACGTG CAC^GTG BpmI 983 CTGGAG CTGGAG(16/14) BsaAI 674 CACGTG [CT]AC^GT[GA] BsaI 502 GAGACC GGTCTC(1/5) BsaJI 777 CCTTGG C^C[ACGT][ACGT]GG BsgI 368 CTGCAC GTGCAG(16/14) BsmFI 712 GTCCC GGGAC(10/14) BssKI 39 CCTGG ^CC[ACGT]GG BstNI 39 CCTGG CC^[AT]GG Cac8I 515 GCAAGC GC[ACGT]^[ACGT]GC Cfr13I 417 GGCCC G^G[ACGT]CC DpnII 753 GATC ^GATC Eco47III 452 AGCGCT AGC^GCT EcoRI 556 GAATTC G^AATTC EcoRII 39 CCTGG ^CC[AT]GG EcoT14I 777 CCTTGG C^C[AT][AT]GG FauI 714 CCCGC CCCGC(4/6) HphI 739 GGTGA GGTGA(8/7) MboI 753 GATC ^GATC MfeI 785 CAATTG C^AATTG MunI 785 CAATTG C^AATTG MvaI 39 CCTGG CC^[AT]GG NdeII 753 GATC ^GATC PleI 318 GAGTC GAGTC(4/5) PmaCI 674 CACGTG CAC^GTG PmlI 674 CACGTG CAC^GTG PvuII 973 CAGCTG CAG^CTG Sau3AI 753 GATC ^GATC Sau96I 417 GGCCC G^G[ACGT]CC ScaI 876 AGTACT AGT^ACT ScrFI 39 CCTGG CC^[ACGT]GG SspI 100 AATATT AAT^ATT StuI 36 AGGCCT AGG^CCT StyI 777 CCTTGG C^C[AT][AT]GG TaqI 930 TCGA T^CGA Tsp45I 854 GTGAC ^GT[GC]AC TthHB8I 930 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 715 CCGC CCGC(-3/-1) 172 GCGG AcsI 556 GAATTC [GA]^AATT[CT] 598 AAATTC AfaI 472 GTAC GT^AC 877 GTAC ApoI 556 GAATTC [GA]^AATT[CT] 598 AAATTC Asp700I 439 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 486 GAAGCCCTTC BbsI 189 GAAGAC GAAGAC(2/6) 239 GTCTTC BpuAI 189 GAAGAC GAAGAC(2/6) 239 GTCTTC BseRI 383 CTCCTC GAGGAG(10/8) 247 GAGGAG BsmAI 502 GAGAC GTCTC(1/5) 379 GTCTC CfoI 453 GCGC GCG^C 668 GCGC HaeII 452 AGCGCT [GA]GCGC^[CT] 667 AGCGCC HhaI 453 GCGC GCG^C 668 GCGC HinP1I 453 GCGC G^CGC 668 GCGC MspA1I 715 CCGCTG C[AC]G^C[GT]G 973 CAGCTG NlaIII 388 CATG CATG^ 532 CATG PstI 179 CTGCAG CTGCA^G 332 CTGCAG RsaI 472 GTAC GT^AC 877 GTAC SfaNI 685 GATGC GCATC(5/9) 328 GCATC XmnI 439 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 486 GAAGCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AluI BbvI BsrI DdeI Fnu4HI HaeIII HinfI ItaI MaeII MaeIII MboII MnlI MseI MwoI SfcI TfiI Tru9I TseI Tsp509I TspRI