Restriction map for
pFN21AE3891
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FokI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MamI
MflI MluI MluNI MroI MscI
MslI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 675 ACGTGT A^C[GA][CT]GT
Aor51HI 452 AGCGCT AGC^GCT
BbrPI 674 CACGTG CAC^GTG
BpmI 983 CTGGAG CTGGAG(16/14)
BsaAI 674 CACGTG [CT]AC^GT[GA]
BsaI 502 GAGACC GGTCTC(1/5)
BsaJI 777 CCTTGG C^C[ACGT][ACGT]GG
BsgI 368 CTGCAC GTGCAG(16/14)
BsmFI 712 GTCCC GGGAC(10/14)
BssKI 39 CCTGG ^CC[ACGT]GG
BstNI 39 CCTGG CC^[AT]GG
Cac8I 515 GCAAGC GC[ACGT]^[ACGT]GC
Cfr13I 417 GGCCC G^G[ACGT]CC
DpnII 753 GATC ^GATC
Eco47III 452 AGCGCT AGC^GCT
EcoRI 556 GAATTC G^AATTC
EcoRII 39 CCTGG ^CC[AT]GG
EcoT14I 777 CCTTGG C^C[AT][AT]GG
FauI 714 CCCGC CCCGC(4/6)
HphI 739 GGTGA GGTGA(8/7)
MboI 753 GATC ^GATC
MfeI 785 CAATTG C^AATTG
MunI 785 CAATTG C^AATTG
MvaI 39 CCTGG CC^[AT]GG
NdeII 753 GATC ^GATC
PleI 318 GAGTC GAGTC(4/5)
PmaCI 674 CACGTG CAC^GTG
PmlI 674 CACGTG CAC^GTG
PvuII 973 CAGCTG CAG^CTG
Sau3AI 753 GATC ^GATC
Sau96I 417 GGCCC G^G[ACGT]CC
ScaI 876 AGTACT AGT^ACT
ScrFI 39 CCTGG CC^[ACGT]GG
SspI 100 AATATT AAT^ATT
StuI 36 AGGCCT AGG^CCT
StyI 777 CCTTGG C^C[AT][AT]GG
TaqI 930 TCGA T^CGA
Tsp45I 854 GTGAC ^GT[GC]AC
TthHB8I 930 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 715 CCGC CCGC(-3/-1)
172 GCGG
AcsI 556 GAATTC [GA]^AATT[CT]
598 AAATTC
AfaI 472 GTAC GT^AC
877 GTAC
ApoI 556 GAATTC [GA]^AATT[CT]
598 AAATTC
Asp700I 439 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
486 GAAGCCCTTC
BbsI 189 GAAGAC GAAGAC(2/6)
239 GTCTTC
BpuAI 189 GAAGAC GAAGAC(2/6)
239 GTCTTC
BseRI 383 CTCCTC GAGGAG(10/8)
247 GAGGAG
BsmAI 502 GAGAC GTCTC(1/5)
379 GTCTC
CfoI 453 GCGC GCG^C
668 GCGC
HaeII 452 AGCGCT [GA]GCGC^[CT]
667 AGCGCC
HhaI 453 GCGC GCG^C
668 GCGC
HinP1I 453 GCGC G^CGC
668 GCGC
MspA1I 715 CCGCTG C[AC]G^C[GT]G
973 CAGCTG
NlaIII 388 CATG CATG^
532 CATG
PstI 179 CTGCAG CTGCA^G
332 CTGCAG
RsaI 472 GTAC GT^AC
877 GTAC
SfaNI 685 GATGC GCATC(5/9)
328 GCATC
XmnI 439 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
486 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BbvI BsrI DdeI Fnu4HI
HaeIII HinfI ItaI MaeII MaeIII
MboII MnlI MseI MwoI SfcI
TfiI Tru9I TseI Tsp509I TspRI