Restriction map for
pFN21AE5137
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmFI BsmI BsoBI Bsp1286I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI DpnII DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboI MflI MluI MluNI
MroI MscI MseI MslI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacI SacII SalI SapI Sau3AI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
SspI StuI StyI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AocI 415 CCTGAGG CC^T[ACGT]AGG
BanI 328 GGCACC G^G[CT][GA]CC
BbsI 429 GAAGAC GAAGAC(2/6)
BpuAI 429 GAAGAC GAAGAC(2/6)
BsaAI 366 TACGTA [CT]AC^GT[GA]
BseRI 402 CTCCTC GAGGAG(10/8)
BsgI 21 CTGCAC GTGCAG(16/14)
BsmAI 223 GTCTC GTCTC(1/5)
BsmBI 222 CGTCTC CGTCTC(1/5)
Bsp1407I 344 TGTACA T^GTACA
BspMI 422 ACCTGC ACCTGC(4/8)
BsrGI 344 TGTACA T^GTACA
BstF5I 360 GGATG GGATG(2/0)
Bsu36I 415 CCTGAGG CC^T[ACGT]AGG
CpoI 202 CGGACCG CG^G[AT]CCG
Eco81I 415 CCTGAGG CC^T[ACGT]AGG
FauI 180 CCCGC CCCGC(4/6)
FokI 360 GGATG GGATG(9/13)
HapII 358 CCGG C^CGG
HgaI 12 GCGTC GACGC(5/10)
HpaII 358 CCGG C^CGG
HphI 128 TCACC GGTGA(8/7)
MaeII 367 ACGT A^CGT
MfeI 251 CAATTG C^AATTG
MspI 358 CCGG C^CGG
MunI 251 CAATTG C^AATTG
NspI 269 ACATGC [GA]CATG^[CT]
PleI 432 GACTC GAGTC(4/5)
PpuMI 419 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 49 CAGCTG CAG^CTG
RsrII 202 CGGACCG CG^G[AT]CCG
SbfI 302 CCTGCAGG CCTGCA^GG
SfaNI 188 GCATC GCATC(5/9)
SnaBI 366 TACGTA TAC^GTA
Sse8387I 302 CCTGCAGG CCTGCA^GG
SspBI 344 TGTACA T^GTACA
TfiI 143 GAATC G^A[AT]TC
Tsp509I 252 AATT ^AATT
TspRI 264 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 181 CCGC CCGC(-3/-1)
206 CCGC
AluI 50 AGCT AG^CT
392 AGCT
BsaJI 65 CCCAGG C^C[ACGT][ACGT]GG
131 CCCAGG
BsiYI 131 CCCAGGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
404 CCTCCTTTGGG
BslI 131 CCCAGGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
404 CCTCCTTTGGG
BspHI 314 TCATGA T^CATGA
377 TCATGA
BsrI 335 ACTGG ACTGG(1/-1)
263 CCAGT
DraII 298 GGGGCCT [GA]G^G[ACGT]CC[CT]
419 AGGACCT
EcoO109I 298 GGGGCCT [GA]G^G[ACGT]CC[CT]
419 AGGACCT
HinfI 143 GAATC G^A[ACGT]TC
432 GACTC
MaeIII 101 GTCAC ^GT[ACGT]AC
266 GTGAC
MboII 429 GAAGA GAAGA(8/7)
146 TCTTC
MspA1I 49 CAGCTG C[AC]G^C[GT]G
181 CCGCTG
NlaIV 298 GGGGCC GG[ACGT]^[ACGT]CC
328 GGCACC
PstI 303 CTGCAG CTGCA^G
424 CTGCAG
RcaI 314 TCATGA T^CATGA
377 TCATGA
Tsp45I 101 GTCAC ^GT[GC]AC
266 GTGAC
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI AlwNI AvaII BbvI BssKI
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI HaeIII ItaI MnlI MvaI
NlaIII RsaI Sau96I ScrFI SfcI
TseI