Restriction map for
pFN21AE3163
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BclI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstUI BstXI BstZ17I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT14I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SphI Sse8387I SspI StuI StyI
SwaI Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1118 GGTACC G^GTACC
AlwNI 981 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 526 CCTAAGG CC^T[ACGT]AGG
Asp718I 1118 GGTACC G^GTACC
AspI 828 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 30 CCCGGG C^[CT]CG[GA]G
BamHI 591 GGATCC G^GATCC
BanI 1118 GGTACC G^G[CT][GA]CC
BbsI 459 GTCTTC GAAGAC(2/6)
BcgI 578 CGAGTGAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 920 GTATCC GTATCC(6/5)
BpmI 217 CTCCAG CTGGAG(16/14)
BpuAI 459 GTCTTC GAAGAC(2/6)
BsaAI 1087 TACGTA [CT]AC^GT[GA]
BsmBI 833 CGTCTC CGTCTC(1/5)
BsoBI 30 CCCGGG C^[CT]CG[GA]G
Bsp1407I 245 TGTACA T^GTACA
BspHI 408 TCATGA T^CATGA
BsrGI 245 TGTACA T^GTACA
BssSI 839 CACGAG CACGAG(-5/-1)
BstAPI 17 GCAAACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 550 GGTGACC G^GT[ACGT]ACC
BstF5I 546 GGATG GGATG(2/0)
BstPI 550 GGTGACC G^GT[ACGT]ACC
Bsu36I 526 CCTAAGG CC^T[ACGT]AGG
DraII 399 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 526 CCTAAGG CC^T[ACGT]AGG
EcoO109I 399 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 550 GGTGACC G^GT[ACGT]ACC
EcoT22I 618 ATGCAT ATGCA^T
FokI 546 GGATG GGATG(9/13)
HaeIII 57 GGCC GG^CC
HapII 31 CCGG C^CGG
HgaI 983 GACGC GACGC(5/10)
HpaII 31 CCGG C^CGG
KpnI 1118 GGTACC GGTAC^C
MseI 643 TTAA T^TAA
MslI 864 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 31 CCGG C^CGG
NsiI 618 ATGCAT ATGCA^T
NspI 711 GCATGT [GA]CATG^[CT]
PflFI 828 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 399 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 412 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 441 CTGCAG CTGCA^G
RcaI 408 TCATGA T^CATGA
SmaI 30 CCCGGG CCC^GGG
SnaBI 1087 TACGTA TAC^GTA
SpeI 646 ACTAGT A^CTAGT
SspBI 245 TGTACA T^GTACA
Tru9I 643 TTAA T^TAA
Tth111I 828 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 942 CCAAGTAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 30 CCCGGG C^CCGGG
XmaI 30 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 15 CCGC CCGC(-3/-1)
1053 GCGG
AfaI 246 GTAC GT^AC
1119 GTAC
AvaII 400 GGACC G^G[AT]CC
827 GGACC
BcnI 30 CCCGG CC^[GC]GG
31 CCGGG
BfaI 187 CTAG C^TAG
647 CTAG
BsaJI 30 CCCGGG C^C[ACGT][ACGT]GG
53 CCTCGG
BsmFI 73 GGGAC GGGAC(10/14)
826 GGGAC
BsrI 432 ACTGG ACTGG(1/-1)
565 ACTGG
BstNI 219 CCAGG CC^[AT]GG
924 CCAGG
BstYI 162 GGATCT [GA]^GATC[CT]
591 GGATCC
Cfr13I 400 GGACC G^G[ACGT]CC
827 GGACC
EarI 532 GAAGAG CTCTTC(1/4)
706 GAAGAG
EcoRI 450 GAATTC G^AATTC
1056 GAATTC
EcoRII 219 CCAGG ^CC[AT]GG
924 CCAGG
HphI 550 GGTGA GGTGA(8/7)
12 TCACC
Ksp632I 532 GAAGAG CTCTTC(1/4)
706 GAAGAG
MaeI 187 CTAG C^TAG
647 CTAG
MfeI 517 CAATTG C^AATTG
999 CAATTG
MflI 162 GGATCT [GA]^GATC[CT]
591 GGATCC
MspA1I 80 CAGCTG C[AC]G^C[GT]G
1044 CAGCTG
MunI 517 CAATTG C^AATTG
999 CAATTG
MvaI 219 CCAGG CC^[AT]GG
924 CCAGG
NciI 30 CCCGG CC^[GC]GG
31 CCGGG
PleI 333 GACTC GAGTC(4/5)
251 GAGTC
PvuII 80 CAGCTG CAG^CTG
1044 CAGCTG
RsaI 246 GTAC GT^AC
1119 GTAC
SapI 532 GAAGAGC GCTCTTC(1/4)
706 GAAGAGC
Sau96I 400 GGACC G^G[ACGT]CC
827 GGACC
SfcI 77 CTACAG C^T[GA][CT]AG
441 CTGCAG
TaqI 448 TCGA T^CGA
577 TCGA
TfiI 559 GAATC G^A[AT]TC
913 GAATC
TthHB8I 448 TCGA T^CGA
577 TCGA
XhoII 162 GGATCT [GA]^GATC[CT]
591 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BsiYI BslI BsmAI BssKI DdeI
DpnII Fnu4HI HinfI ItaI MaeII
MaeIII MboI MboII MnlI MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
SfaNI TseI Tsp45I Tsp509I TspRI