Restriction map for
pFN21AE5783
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# NO CUT ENZYMES ( 112 enzymes) #
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Acc65I AccII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspLU11I
BsrBI BsrFI BsrGI BssHII BstUI
BstXI CelII CfoI Cfr10I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 211 GACGTC GACGT^C
AccI 1337 GTATAC GT^[AC][GT]AC
AccIII 12 TCCGGA T^CCGGA
AocI 358 CCTGAGG CC^T[ACGT]AGG
BalI 119 TGGCCA TGG^CCA
BanII 994 GGGCTC G[GA]GC[CT]^C
BglI 52 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1931 CTGGAG CTGGAG(16/14)
BsaI 836 GAGACC GGTCTC(1/5)
BseAI 12 TCCGGA T^CCGGA
BspDI 1241 ATCGAT AT^CGAT
BspEI 12 TCCGGA T^CCGGA
BspHI 1793 TCATGA T^CATGA
BspMI 1230 GCAGGT ACCTGC(4/8)
Bst1107I 1337 GTATAC GTA^TAC
BstBI 416 TTCGAA TT^CGAA
BstEII 1963 GGTTACC G^GT[ACGT]ACC
BstPI 1963 GGTTACC G^GT[ACGT]ACC
BstZ17I 1337 GTATAC GTA^TAC
Bsu36I 358 CCTGAGG CC^T[ACGT]AGG
ClaI 1241 ATCGAT AT^CGAT
DraIII 145 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1114 GACGCTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 697 CCATGG C^C[GA][CT]GG
Eco81I 358 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1963 GGTTACC G^GT[ACGT]ACC
EcoRI 424 GAATTC G^AATTC
FauI 990 GCGGG CCCGC(4/6)
MfeI 1370 CAATTG C^AATTG
MluNI 119 TGGCCA TGG^CCA
MroI 12 TCCGGA T^CCGGA
MscI 119 TGGCCA TGG^CCA
MunI 1370 CAATTG C^AATTG
NcoI 697 CCATGG C^CATGG
NspI 986 GCATGC [GA]CATG^[CT]
NspV 416 TTCGAA TT^CGAA
PpuMI 871 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1793 TCATGA T^CATGA
SapI 1788 GCTCTTC GCTCTTC(1/4)
ScaI 1293 AGTACT AGT^ACT
SfiI 51 GGCCACCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 416 TTCGAA TT^CGAA
SmlI 536 CTCAAG C^T[CT][GA]AG
SphI 986 GCATGC GCATG^C
SwaI 491 ATTTAAAT ATTT^AAAT
Tsp45I 1084 GTCAC ^GT[GC]AC
XcmI 122 CCAACAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 211 GACGTC G[GA]^CG[CT]C
231 GGCGTC
Asp700I 1239 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1555 GAAGTCTTTC
AvaII 243 GGACC G^G[AT]CC
872 GGACC
BbsI 705 GTCTTC GAAGAC(2/6)
1352 GTCTTC
BbvCI 1300 CCTCAGC CCTCAGC(-5/-2)
1573 GCTGAGG
BcnI 56 CCGGG CC^[GC]GG
875 CCCGG
BpuAI 705 GTCTTC GAAGAC(2/6)
1352 GTCTTC
BsaHI 211 GACGTC G[GA]^CG[CT]C
231 GGCGTC
BsaWI 12 TCCGGA [AT]^CCGG[AT]
162 ACCGGA
BsgI 1511 CTGCAC GTGCAG(16/14)
761 GTGCAG
BssSI 686 CACGAG CACGAG(-5/-1)
1742 CACGAG
BstYI 114 GGATCT [GA]^GATC[CT]
603 AGATCC
Cac8I 986 GCATGC GC[ACGT]^[ACGT]GC
1777 GCAGGC
DraI 492 TTTAAA TTT^AAA
1608 TTTAAA
DraII 871 GGGACCC [GA]G^G[ACGT]CC[CT]
1913 AGGGCCT
EaeI 119 TGGCCA [CT]^GGCC[GA]
1007 CGGCCA
EcoO109I 871 GGGACCC [GA]G^G[ACGT]CC[CT]
1913 AGGGCCT
HgaI 1114 GACGC GACGC(5/10)
232 GCGTC
Hin1I 211 GACGTC G[GA]^CG[CT]C
231 GGCGTC
MaeII 212 ACGT A^CGT
470 ACGT
MflI 114 GGATCT [GA]^GATC[CT]
603 AGATCC
NciI 56 CCGGG CC^[GC]GG
875 CCCGG
PflMI 1885 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2024 CCAAAGAATGG
PleI 740 GACTC GAGTC(4/5)
2016 GACTC
PstI 1688 CTGCAG CTGCA^G
1750 CTGCAG
SspI 289 AATATT AAT^ATT
745 AATATT
Van91I 1885 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2024 CCAAAGAATGG
XhoII 114 GGATCT [GA]^GATC[CT]
603 AGATCC
XmnI 1239 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1555 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BbvI BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I