Restriction map for
pFN21AE3033
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII AccII AccIII AciI AclI
AflII AgeI AhdI AlwI AlwNI
ApaI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaJI BseAI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DpnII
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FauI FbaI FseI FspI HaeIII
HincII HindII HindIII HpaI KasI
KspI MaeI MaeII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StuI StyI Tsp45I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 76 enzymes) #
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Acc65I 136 GGTACC G^GTACC
AccI 115 GTAGAC GT^[AC][GT]AC
AcyI 3 GGCGTC G[GA]^CG[CT]C
AfaI 137 GTAC GT^AC
AflIII 400 ACATGT A^C[GA][CT]GT
AluI 352 AGCT AG^CT
Alw44I 128 GTGCAC G^TGCAC
AocI 205 CCTCAGG CC^T[ACGT]AGG
Aor51HI 303 AGCGCT AGC^GCT
ApaLI 128 GTGCAC G^TGCAC
Asp718I 136 GGTACC G^GTACC
AspHI 128 GTGCAC G[AT]GC[AT]^C
AvaII 296 GGTCC G^G[AT]CC
BanI 136 GGTACC G^G[CT][GA]CC
BbsI 289 GAAGAC GAAGAC(2/6)
BbvI 353 GCTGC GCAGC(8/12)
BmyI 128 GTGCAC G[AGT]GC[ACT]^C
BpuAI 289 GAAGAC GAAGAC(2/6)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaWI 375 ACCGGA [AT]^CCGG[AT]
BseRI 203 CTCCTC GAGGAG(10/8)
BsiHKAI 128 GTGCAC G[AT]GC[AT]^C
BsiYI 55 CCACCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 55 CCACCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 366 GAGAC GTCTC(1/5)
BsmBI 366 GAGACG CGTCTC(1/5)
BsmFI 297 GTCCC GGGAC(10/14)
Bsp1286I 128 GTGCAC G[AGT]GC[ACT]^C
BspHI 438 TCATGA T^CATGA
BspLU11I 400 ACATGT A^CATGT
BsrDI 257 GCAATG GCAATG(2/0)
BstF5I 241 CATCC GGATG(2/0)
Bsu36I 205 CCTCAGG CC^T[ACGT]AGG
Cac8I 341 GCATGC GC[ACGT]^[ACGT]GC
CfoI 304 GCGC GCG^C
Cfr13I 296 GGTCC G^G[ACGT]CC
DraI 172 TTTAAA TTT^AAA
EarI 81 GAAGAG CTCTTC(1/4)
Eco47III 303 AGCGCT AGC^GCT
Eco81I 205 CCTCAGG CC^T[ACGT]AGG
EcoRV 188 GATATC GAT^ATC
Fnu4HI 353 GCTGC GC^[ACGT]GC
FokI 241 CATCC GGATG(9/13)
HaeII 303 AGCGCT [GA]GCGC^[CT]
HapII 376 CCGG C^CGG
HgaI 4 GCGTC GACGC(5/10)
HhaI 304 GCGC GCG^C
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinP1I 304 GCGC G^CGC
HpaII 376 CCGG C^CGG
HphI 223 GGTGA GGTGA(8/7)
ItaI 353 GCTGC GC^[ACGT]GC
KpnI 136 GGTACC GGTAC^C
Ksp632I 81 GAAGAG CTCTTC(1/4)
MaeIII 404 GTAAC ^GT[ACGT]AC
MslI 241 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 351 CAGCTG C[AC]G^C[GT]G
MspI 376 CCGG C^CGG
MwoI 332 GCATTATTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 55 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 199 GACTC GAGTC(4/5)
PvuII 351 CAGCTG CAG^CTG
RcaI 438 TCATGA T^CATGA
RsaI 137 GTAC GT^AC
Sau96I 296 GGTCC G^G[ACGT]CC
SexAI 132 ACCAGGT A^CC[AT]GGT
SfcI 22 CTACAG C^T[GA][CT]AG
SphI 341 GCATGC GCATG^C
SspI 229 AATATT AAT^ATT
SwaI 171 ATTTAAAT ATTT^AAAT
TaqI 186 TCGA T^CGA
TfiI 411 GAATC G^A[AT]TC
TseI 353 GCTGC G^C[AT]GC
TspRI 106 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 186 TCGA T^CGA
Van91I 55 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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Asp700I 154 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
226 GAAAATATTC
BsrI 220 ACTGG ACTGG(1/-1)
293 ACTGG
BssKI 61 CCTGG ^CC[ACGT]GG
133 CCAGG
BstNI 61 CCTGG CC^[AT]GG
133 CCAGG
EcoRII 61 CCTGG ^CC[AT]GG
133 CCAGG
HinfI 199 GACTC G^A[ACGT]TC
411 GAATC
MboII 81 GAAGA GAAGA(8/7)
289 GAAGA
MseI 73 TTAA T^TAA
173 TTAA
MvaI 61 CCTGG CC^[AT]GG
133 CCAGG
NlaIV 136 GGTACC GG[ACGT]^[ACGT]CC
296 GGTCCC
NspI 341 GCATGC [GA]CATG^[CT]
400 ACATGT
ScrFI 61 CCTGG CC^[ACGT]GG
133 CCAGG
Tru9I 73 TTAA T^TAA
173 TTAA
XmnI 154 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
226 GAAAATATTC
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
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AcsI ApoI DdeI MnlI NlaIII
Tsp509I