Restriction map for
pFN21AE1276
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BlnI BpmI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FauI FbaI FseI FspI
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KspI MluI MluNI
MroI MscI MspI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1648 GGTACC G^GTACC
AccII 14 CGCG CG^CG
AhdI 1942 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 403 CCTGAGG CC^T[ACGT]AGG
Asp718I 1648 GGTACC G^GTACC
AspEI 1942 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1648 GGTACC G^G[CT][GA]CC
BanII 2243 GAGCTC G[GA]GC[CT]^C
BglI 1024 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1636 AGATCT A^GATCT
BlpI 994 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 994 GCTCAGC GC^T[ACGT]AGC
BsgI 635 GTGCAG GTGCAG(16/14)
BsmAI 1078 GAGAC GTCTC(1/5)
BspDI 224 ATCGAT AT^CGAT
BspHI 54 TCATGA T^CATGA
BspMI 1818 ACCTGC ACCTGC(4/8)
BstAPI 484 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 14 CGCG CG^CG
BstXI 319 CCAAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 403 CCTGAGG CC^T[ACGT]AGG
CelII 994 GCTCAGC GC^T[ACGT]AGC
ClaI 224 ATCGAT AT^CGAT
DraII 261 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 1022 TGGCCG [CT]^GGCC[GA]
Eam1105I 1942 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 403 CCTGAGG CC^T[ACGT]AGG
EcoNI 1800 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 261 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 2204 GATATC GAT^ATC
HaeII 1422 GGCGCT [GA]GCGC^[CT]
HincII 1923 GTTGAC GT[CT]^[GA]AC
HindII 1923 GTTGAC GT[CT]^[GA]AC
KpnI 1648 GGTACC GGTAC^C
MfeI 720 CAATTG C^AATTG
MspA1I 830 CAGCTG C[AC]G^C[GT]G
MunI 720 CAATTG C^AATTG
MvnI 14 CGCG CG^CG
NcoI 1211 CCATGG C^CATGG
NdeI 649 CATATG CA^TATG
PpuMI 261 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 830 CAGCTG CAG^CTG
RcaI 54 TCATGA T^CATGA
SacI 2243 GAGCTC GAGCT^C
SfcI 1106 CTGTAG C^T[GA][CT]AG
SmlI 811 CTTGAG C^T[CT][GA]AG
TaqI 225 TCGA T^CGA
TthHB8I 225 TCGA T^CGA
XbaI 1083 TCTAGA T^CTAGA
XcmI 1010 CCACAAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 1850 CCGC CCGC(-3/-1)
465 GCGG
AseI 1182 ATTAAT AT^TAAT
1977 ATTAAT
AsnI 1182 ATTAAT AT^TAAT
1977 ATTAAT
AspHI 946 GAGCAC G[AT]GC[AT]^C
2243 GAGCTC
AvaII 262 GGTCC G^G[AT]CC
1711 GGACC
BmyI 946 GAGCAC G[AGT]GC[ACT]^C
2243 GAGCTC
BseRI 1565 CTCCTC GAGGAG(10/8)
433 GAGGAG
BsiHKAI 946 GAGCAC G[AT]GC[AT]^C
2243 GAGCTC
BsmFI 119 GGGAC GGGAC(10/14)
1728 GTCCC
BsmI 1216 GAATGC GAATGC(1/-1)
1680 GAATGC
Bsp1286I 946 GAGCAC G[AGT]GC[ACT]^C
2243 GAGCTC
DsaI 1025 CCGTGG C^C[GA][CT]GG
1211 CCATGG
EcoT14I 265 CCTTGG C^C[AT][AT]GG
1211 CCATGG
HaeIII 317 GGCC GG^CC
1023 GGCC
HphI 709 TCACC GGTGA(8/7)
1006 TCACC
MaeII 1121 ACGT A^CGT
1229 ACGT
MslI 55 CATGATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
949 CACACTAGTG
PleI 174 GACTC GAGTC(4/5)
1080 GACTC
PshBI 1182 ATTAAT AT^TAAT
1977 ATTAAT
ScaI 2212 AGTACT AGT^ACT
2233 AGTACT
SpeI 952 ACTAGT A^CTAGT
1878 ACTAGT
StyI 265 CCTTGG C^C[AT][AT]GG
1211 CCATGG
Tsp45I 708 GTCAC ^GT[GC]AC
1118 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI Asp700I BbsI BbvI BfaI
BpuAI BsaBI BsaJI BsiYI BslI
BspLU11I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
Fnu4HI FokI HhaI HinP1I HinfI
ItaI Ksp632I MaeI MaeIII MamI
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp509I
TspRI XhoII XmnI