##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AciI          969  GCGG                 CCGC(-3/-1)
AcsI          360  AAATTT               [GA]^AATT[CT]
AflIII        851  ACATGT               A^C[GA][CT]GT
ApoI          360  AAATTT               [GA]^AATT[CT]
Asp700I       184  GAATGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         743  GGTCC                G^G[AT]CC
BamHI         221  GGATCC               G^GATCC
BbsI          316  GAAGAC               GAAGAC(2/6)
BciVI         241  GTATCC               GTATCC(6/5)
BclI          662  TGATCA               T^GATCA
BfaI            4  CTAG                 C^TAG
BpmI           20  CTCCAG               CTGGAG(16/14)
BpuAI         316  GAAGAC               GAAGAC(2/6)
BsgI          537  CTGCAC               GTGCAG(16/14)
BsmAI         708  GAGAC                GTCTC(1/5)
BsmI          334  GCATTC               GAATGC(1/-1)
BspHI         266  TCATGA               T^CATGA
BspLU11I      851  ACATGT               A^CATGT
BsrI          463  ACTGG                ACTGG(1/-1)
BstAPI        518  GCATGTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        672  CATCC                GGATG(2/0)
BstXI         997  CCAAAAGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         221  GGATCC               [GA]^GATC[CT]
Cac8I         913  GCCAGC               GC[ACGT]^[ACGT]GC
EarI          956  GAAGAG               CTCTTC(1/4)
EcoT22I       332  ATGCAT               ATGCA^T
FbaI          662  TGATCA               T^GATCA
FokI          672  CATCC                GGATG(9/13)
HaeIII        777  GGCC                 GG^CC
HgaI          319  GACGC                GACGC(5/10)
HphI          101  TCACC                GGTGA(8/7)
Ksp632I       956  GAAGAG               CTCTTC(1/4)
MaeI            4  CTAG                 C^TAG
MflI          221  GGATCC               [GA]^GATC[CT]
MspA1I        179  CAGCTG               C[AC]G^C[GT]G
NsiI          332  ATGCAT               ATGCA^T
PstI           71  CTGCAG               CTGCA^G
PvuII         179  CAGCTG               CAG^CTG
RcaI          266  TCATGA               T^CATGA
SapI          956  GAAGAGC              GCTCTTC(1/4)
SfaNI         527  GCATC                GCATC(5/9)
SfcI           71  CTGCAG               C^T[GA][CT]AG
SmlI          703  CTCAAG               C^T[CT][GA]AG
TaqI          981  TCGA                 T^CGA
TfiI          933  GATTC                G^A[AT]TC
TspRI         540  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       981  TCGA                 T^CGA
XhoII         221  GGATCC               [GA]^GATC[CT]
XmnI          184  GAATGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BseRI         573  CTCCTC               GAGGAG(10/8)
              656  CTCCTC              
BsrDI         147  CATTGC               GCAATG(2/0)
              731  GCAATG              
Cfr13I        743  GGTCC                G^G[ACGT]CC
              776  GGGCC               
PleI          654  GACTC                GAGTC(4/5)
             1008  GACTC               
Sau96I        743  GGTCC                G^G[ACGT]CC
              776  GGGCC               
Tsp45I        512  GTCAC                ^GT[GC]AC
              652  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AluI        AlwI        BbvI        BsaJI       BsiYI       
BslI        BssKI       BstNI       DdeI        DpnII       
EcoRII      Fnu4HI      HinfI       ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
Sau3AI      ScrFI       Tru9I       TseI        Tsp509I