Restriction map for
pFN21AE5988
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# NO CUT ENZYMES ( 144 enzymes) #
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AccI AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HincII HindII HindIII HinfI
HpaI KasI Ksp632I KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 508 GACGTC GACGT^C
Acc65I 462 GGTACC G^GTACC
AcyI 508 GACGTC G[GA]^CG[CT]C
Asp700I 699 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 462 GGTACC G^GTACC
AspHI 482 GAGCTC G[AT]GC[AT]^C
AvaI 479 CCCGAG C^[CT]CG[GA]G
BalI 365 TGGCCA TGG^CCA
BamHI 674 GGATCC G^GATCC
BanI 462 GGTACC G^G[CT][GA]CC
BanII 482 GAGCTC G[GA]GC[CT]^C
BbsI 736 GTCTTC GAAGAC(2/6)
BbvCI 615 CCTCAGC CCTCAGC(-5/-2)
BcnI 405 CCGGG CC^[GC]GG
BfaI 454 CTAG C^TAG
BglII 203 AGATCT A^GATCT
BpmI 199 CTGGAG CTGGAG(16/14)
BpuAI 736 GTCTTC GAAGAC(2/6)
BsaHI 508 GACGTC G[GA]^CG[CT]C
BsaI 719 GGTCTC GGTCTC(1/5)
BseRI 45 CTCCTC GAGGAG(10/8)
BsiHKAI 482 GAGCTC G[AT]GC[AT]^C
BsmAI 720 GTCTC GTCTC(1/5)
BsoBI 479 CCCGAG C^[CT]CG[GA]G
BstXI 420 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 384 GCGC GCG^C
DraII 65 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 790 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 244 GACCTGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 365 TGGCCA [CT]^GGCC[GA]
EcoNI 924 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 65 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 580 GAATTC G^AATTC
FauI 716 GCGGG CCCGC(4/6)
HapII 405 CCGG C^CGG
HhaI 384 GCGC GCG^C
Hin1I 508 GACGTC G[GA]^CG[CT]C
HinP1I 384 GCGC G^CGC
HpaII 405 CCGG C^CGG
KpnI 462 GGTACC GGTAC^C
MaeI 454 CTAG C^TAG
MaeII 509 ACGT A^CGT
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
MseI 884 TTAA T^TAA
MspI 405 CCGG C^CGG
NciI 405 CCGGG CC^[GC]GG
PshAI 606 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 482 GAGCTC GAGCT^C
Tru9I 884 TTAA T^TAA
XmnI 699 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 672 GCGG CCGC(-3/-1)
716 GCGG
AcsI 25 GAATTT [GA]^AATT[CT]
580 GAATTC
AlwI 675 GATCC GGATC(4/5)
674 GGATC
AlwNI 687 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
910 CAGAGGCTG
ApoI 25 GAATTT [GA]^AATT[CT]
580 GAATTC
BmyI 226 GTGCCC G[AGT]GC[ACT]^C
482 GAGCTC
BsmFI 587 GGGAC GGGAC(10/14)
253 GTCCC
Bsp1286I 226 GTGCCC G[AGT]GC[ACT]^C
482 GAGCTC
BstEII 210 GGTAACC G^GT[ACGT]ACC
630 GGTGACC
BstF5I 333 CATCC GGATG(2/0)
233 GGATG
BstPI 210 GGTAACC G^GT[ACGT]ACC
630 GGTGACC
BstYI 203 AGATCT [GA]^GATC[CT]
674 GGATCC
DsaI 458 CCATGG C^C[GA][CT]GG
501 CCATGG
EcoO65I 210 GGTAACC G^GT[ACGT]ACC
630 GGTGACC
FokI 333 CATCC GGATG(9/13)
233 GGATG
MflI 203 AGATCT [GA]^GATC[CT]
674 GGATCC
MslI 129 CATAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
393 CACTCTGATG
MspA1I 421 CAGCTG C[AC]G^C[GT]G
690 CAGCTG
NcoI 458 CCATGG C^CATGG
501 CCATGG
PvuII 421 CAGCTG CAG^CTG
690 CAGCTG
SfaNI 234 GATGC GCATC(5/9)
906 GATGC
StuI 775 AGGCCT AGG^CCT
921 AGGCCT
XhoII 203 AGATCT [GA]^GATC[CT]
674 GGATCC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaJI
BsiYI BslI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI HaeIII HphI ItaI
MaeIII MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TseI Tsp45I Tsp509I TspRI