Restriction map for
pFN21AE3162
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiHKAI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MaeIII MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 191 GGATC GGATC(4/5)
AlwNI 706 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 524 AGCGCT AGC^GCT
Asp700I 522 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 355 GTCTTC GAAGAC(2/6)
BglII 764 AGATCT A^GATCT
BpmI 310 CTGGAG CTGGAG(16/14)
BpuAI 355 GTCTTC GAAGAC(2/6)
BsaBI 942 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 647 CGGCCG CG[GA][CT]^CG
BsmI 743 GAATGC GAATGC(1/-1)
BsrBI 1024 CCGCTC CCGCTC(-3/-3)
BsrI 792 ACTGG ACTGG(1/-1)
BssHII 608 GCGCGC G^CGCGC
BstAPI 483 GCAGTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 764 AGATCT [GA]^GATC[CT]
EagI 647 CGGCCG C^GGCCG
EclXI 647 CGGCCG C^GGCCG
Eco47III 524 AGCGCT AGC^GCT
Eco52I 647 CGGCCG C^GGCCG
EcoNI 309 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 57 GCGTC GACGC(5/10)
HinfI 980 GACTC G^A[ACGT]TC
KspI 903 CCGCGG CCGC^GG
MamI 942 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 764 AGATCT [GA]^GATC[CT]
PleI 980 GACTC GAGTC(4/5)
PvuII 855 CAGCTG CAG^CTG
SacII 903 CCGCGG CCGC^GG
SnaBI 1126 TACGTA TAC^GTA
XhoII 764 AGATCT [GA]^GATC[CT]
XmnI 522 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 27 GGGCCC GGGCC^C
65 GGGCCC
AvaI 61 CCCGGG C^[CT]CG[GA]G
632 CCCGGG
BbrPI 385 CACGTG CAC^GTG
987 CACGTG
BfaI 256 CTAG C^TAG
403 CTAG
BglI 428 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
677 GCCCCAGGGGC
BseRI 264 CTCCTC GAGGAG(10/8)
367 CTCCTC
BsgI 286 CTGCAC GTGCAG(16/14)
1120 GTGCAG
BsoBI 61 CCCGGG C^[CT]CG[GA]G
632 CCCGGG
BspMI 245 ACCTGC ACCTGC(4/8)
323 ACCTGC
BsrFI 97 GCCGGC [GA]^CCGG[CT]
132 GCCGGC
Cfr10I 97 GCCGGC [GA]^CCGG[CT]
132 GCCGGC
DsaI 672 CCGTGG C^C[GA][CT]GG
903 CCGCGG
EaeI 647 CGGCCG [CT]^GGCC[GA]
819 CGGCCA
MaeI 256 CTAG C^TAG
403 CTAG
NaeI 97 GCCGGC GCC^GGC
132 GCCGGC
NgoMIV 97 GCCGGC G^CCGGC
132 GCCGGC
PmaCI 385 CACGTG CAC^GTG
987 CACGTG
PmlI 385 CACGTG CAC^GTG
987 CACGTG
PpuMI 635 GGGTCCC [GA]G^G[AT]CC[CT]
1091 AGGACCT
SmaI 61 CCCGGG CCC^GGG
632 CCCGGG
TspRI 120 CACTG CA[GC]TG[ACGT][ACGT]^
505 CACTG
XmaCI 61 CCCGGG C^CCGGG
632 CCCGGG
XmaI 61 CCCGGG C^CCGGG
632 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AvaII BanI BanII BbeI
BbvI BcnI BmyI BsaAI BsaHI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI TseI Tsp509I