Restriction map for
pFN21AE3429
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AlwNI
AocI Aor51HI AscI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsiEI BsiWI BsmBI BspDI
BspHI BspLU11I BspMI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV FauI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 739 TCCGGA T^CCGGA
AhdI 781 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1510 GTGCAC G^TGCAC
ApaI 888 GGGCCC GGGCC^C
ApaLI 1510 GTGCAC G^TGCAC
AspEI 781 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1679 CTCGAG C^[CT]CG[GA]G
BbsI 1284 GTCTTC GAAGAC(2/6)
BcgI 675 GCAGTACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1703 TGATCA T^GATCA
BglII 219 AGATCT A^GATCT
BpuAI 1284 GTCTTC GAAGAC(2/6)
BsaBI 1684 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 739 TCCGGA T^CCGGA
BsgI 1049 GTGCAG GTGCAG(16/14)
BsmFI 264 GGGAC GGGAC(10/14)
BsoBI 1679 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1240 TGTACA T^GTACA
BspEI 739 TCCGGA T^CCGGA
BsrBI 4 GAGCGG CCGCTC(-3/-3)
BsrGI 1240 TGTACA T^GTACA
BstEII 1783 GGTGACC G^GT[ACGT]ACC
BstF5I 627 CATCC GGATG(2/0)
BstPI 1783 GGTGACC G^GT[ACGT]ACC
DraI 451 TTTAAA TTT^AAA
DrdI 1153 GACTGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 781 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1783 GGTGACC G^GT[ACGT]ACC
EcoT22I 1614 ATGCAT ATGCA^T
FbaI 1703 TGATCA T^GATCA
FokI 627 CATCC GGATG(9/13)
HaeII 827 AGCGCC [GA]GCGC^[CT]
MaeII 1032 ACGT A^CGT
MamI 1684 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 739 TCCGGA T^CCGGA
MslI 1269 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1271 CCATGG C^CATGG
NsiI 1614 ATGCAT ATGCA^T
NspI 1612 GCATGC [GA]CATG^[CT]
PaeR7I 1679 CTCGAG C^TCGAG
SacI 725 GAGCTC GAGCT^C
ScaI 754 AGTACT AGT^ACT
SfaNI 507 GCATC GCATC(5/9)
SphI 1612 GCATGC GCATG^C
SspBI 1240 TGTACA T^GTACA
SspI 1754 AATATT AAT^ATT
TaqI 1680 TCGA T^CGA
TthHB8I 1680 TCGA T^CGA
XhoI 1679 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AseI 1060 ATTAAT AT^TAAT
1087 ATTAAT
AsnI 1060 ATTAAT AT^TAAT
1087 ATTAAT
AvaII 41 GGACC G^G[AT]CC
1393 GGACC
BalI 1008 TGGCCA TGG^CCA
1631 TGGCCA
BanI 1725 GGTGCC G^G[CT][GA]CC
1946 GGCACC
BanII 725 GAGCTC G[GA]GC[CT]^C
888 GGGCCC
BpmI 22 CTCCAG CTGGAG(16/14)
722 CTGGAG
BsaWI 374 TCCGGT [AT]^CCGG[AT]
739 TCCGGA
BseRI 728 CTCCTC GAGGAG(10/8)
136 GAGGAG
BsmI 854 GAATGC GAATGC(1/-1)
1343 GCATTC
BstAPI 507 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1607 GCACGGCATGC
CfoI 19 GCGC GCG^C
828 GCGC
DraII 888 GGGCCCT [GA]G^G[ACGT]CC[CT]
1817 GGGGCCT
DsaI 1271 CCATGG C^C[GA][CT]GG
1418 CCACGG
EaeI 1008 TGGCCA [CT]^GGCC[GA]
1631 TGGCCA
EcoNI 1657 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1821 CCTTTTTGAGG
EcoO109I 888 GGGCCCT [GA]G^G[ACGT]CC[CT]
1817 GGGGCCT
HhaI 19 GCGC GCG^C
828 GCGC
HinP1I 19 GCGC G^CGC
828 GCGC
MluNI 1008 TGGCCA TGG^CCA
1631 TGGCCA
MscI 1008 TGGCCA TGG^CCA
1631 TGGCCA
MspA1I 776 CAGCTG C[AC]G^C[GT]G
1868 CAGCTG
PshBI 1060 ATTAAT AT^TAAT
1087 ATTAAT
PstI 695 CTGCAG CTGCA^G
746 CTGCAG
PvuII 776 CAGCTG CAG^CTG
1868 CAGCTG
Tsp45I 1640 GTGAC ^GT[GC]AC
1784 GTGAC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp509I TspRI XhoII XmnI