Restriction map for
pFN21AE6590
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AgeI 332 ACCGGT A^CCGGT
BanI 463 GGTGCC G^G[CT][GA]CC
BbvI 480 GCAGC GCAGC(8/12)
BfaI 442 CTAG C^TAG
BsaJI 299 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 332 ACCGGT [AT]^CCGG[AT]
BsiYI 274 CCTGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 274 CCTGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 70 GAGAC GTCTC(1/5)
BspMI 269 ACCTGC ACCTGC(4/8)
BsrBI 526 GAGCGG CCGCTC(-3/-3)
BsrDI 128 GCAATG GCAATG(2/0)
BsrFI 332 ACCGGT [GA]^CCGG[CT]
BsrI 97 CCAGT ACTGG(1/-1)
BstAPI 480 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 158 TTCGAA TT^CGAA
BstF5I 134 GGATG GGATG(2/0)
BstXI 293 CCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 387 GCTTGC GC[ACGT]^[ACGT]GC
Cfr10I 332 ACCGGT [GA]^CCGG[CT]
DraII 266 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 237 CTCTTC CTCTTC(1/4)
EcoNI 274 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 266 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 528 GCGGG CCCGC(4/6)
Fnu4HI 480 GCAGC GC^[ACGT]GC
FokI 134 GGATG GGATG(9/13)
HaeIII 183 GGCC GG^CC
HapII 333 CCGG C^CGG
HincII 100 GTTAAC GT[CT]^[GA]AC
HindII 100 GTTAAC GT[CT]^[GA]AC
HpaI 100 GTTAAC GTT^AAC
HpaII 333 CCGG C^CGG
ItaI 480 GCAGC GC^[ACGT]GC
Ksp632I 237 CTCTTC CTCTTC(1/4)
MaeI 442 CTAG C^TAG
MseI 101 TTAA T^TAA
MslI 105 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 398 CCGCTG C[AC]G^C[GT]G
MspI 333 CCGG C^CGG
NspV 158 TTCGAA TT^CGAA
PinAI 332 ACCGGT A^CCGGT
PpuMI 266 GGGACCT [GA]G^G[AT]CC[CT]
PstI 487 CTGCAG CTGCA^G
SfcI 487 CTGCAG C^T[GA][CT]AG
SfuI 158 TTCGAA TT^CGAA
StuI 182 AGGCCT AGG^CCT
TaqI 159 TCGA T^CGA
TfiI 375 GAATC G^A[AT]TC
Tru9I 101 TTAA T^TAA
TseI 480 GCAGC G^C[AT]GC
Tsp509I 365 AATT ^AATT
TspRI 150 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 159 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 398 CCGC CCGC(-3/-1)
528 GCGG
AlwNI 281 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
481 CAGCTACTG
BmyI 152 GTGCCC G[AGT]GC[ACT]^C
464 GTGCCC
BpmI 517 CTCCAG CTGGAG(16/14)
301 CTGGAG
BsgI 359 CTGCAC GTGCAG(16/14)
478 GTGCAG
BsmFI 119 GGGAC GGGAC(10/14)
266 GGGAC
Bsp1286I 152 GTGCCC G[AGT]GC[ACT]^C
464 GTGCCC
DpnII 54 GATC ^GATC
492 GATC
HphI 51 GGTGA GGTGA(8/7)
187 TCACC
MboI 54 GATC ^GATC
492 GATC
MboII 78 GAAGA GAAGA(8/7)
238 TCTTC
MwoI 391 GCCAAAACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
480 GCAGCTACTGC
NdeII 54 GATC ^GATC
492 GATC
NlaIV 266 GGGACC GG[ACGT]^[ACGT]CC
463 GGTGCC
PleI 72 GACTC GAGTC(4/5)
121 GACTC
Sau3AI 54 GATC ^GATC
492 GATC
SfaNI 384 GATGC GCATC(5/9)
355 GCATC
XcmI 290 CCACCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
437 CCAAGCTAGACATGG
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI AvaII BbvCI BssKI BstNI
Cfr13I DdeI EcoRII HinfI MnlI
MvaI NlaIII Sau96I ScrFI