Restriction map for
pFN21AE0984
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvaI AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoT22I FauI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HpaII KasI KpnI KspI MluI
MroI MspA1I MspI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RsaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TfiI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 1271 GCGG CCGC(-3/-1)
AlwNI 452 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 933 ATTAAT AT^TAAT
AsnI 933 ATTAAT AT^TAAT
AspHI 500 GAGCTC G[AT]GC[AT]^C
AspI 1142 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1447 GGTCC G^G[AT]CC
BamHI 1440 GGATCC G^GATCC
BciVI 397 GTATCC GTATCC(6/5)
BclI 569 TGATCA T^GATCA
BglII 1546 AGATCT A^GATCT
BpmI 1184 CTCCAG CTGGAG(16/14)
BsaBI 1696 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 111 GAGACC GGTCTC(1/5)
BseRI 1100 GAGGAG GAGGAG(10/8)
BsiHKAI 500 GAGCTC G[AT]GC[AT]^C
BsmBI 1386 GAGACG CGTCTC(1/5)
BsmFI 461 GGGAC GGGAC(10/14)
Cac8I 1430 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 1447 GGTCC G^G[ACGT]CC
DraII 1446 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1446 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1561 GAATTC G^AATTC
FbaI 569 TGATCA T^GATCA
HgaI 1388 GACGC GACGC(5/10)
HindIII 760 AAGCTT A^AGCTT
HpaI 1365 GTTAAC GTT^AAC
MaeII 1691 ACGT A^CGT
MamI 1696 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 335 CAATTG C^AATTG
MunI 335 CAATTG C^AATTG
NlaIV 1440 GGATCC GG[ACGT]^[ACGT]CC
PflFI 1142 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 485 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1446 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 933 ATTAAT AT^TAAT
PstI 910 CTGCAG CTGCA^G
SacI 500 GAGCTC GAGCT^C
Sau96I 1447 GGTCC G^G[ACGT]CC
SexAI 182 ACCTGGT A^CC[AT]GGT
SfcI 910 CTGCAG C^T[GA][CT]AG
SmlI 719 CTCAAG C^T[CT][GA]AG
SspI 388 AATATT AAT^ATT
Tth111I 1142 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 485 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Asp700I 1165 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1557 GAAAGAATTC
BanII 500 GAGCTC G[GA]GC[CT]^C
690 GAGCCC
BbvI 451 GCAGC GCAGC(8/12)
1324 GCTGC
BmyI 500 GAGCTC G[AGT]GC[ACT]^C
690 GAGCCC
Bsp1286I 500 GAGCTC G[AGT]GC[ACT]^C
690 GAGCCC
BspHI 53 TCATGA T^CATGA
846 TCATGA
BsrDI 1267 CATTGC GCAATG(2/0)
1732 CATTGC
BstF5I 1174 CATCC GGATG(2/0)
1155 GGATG
EcoT14I 18 CCTTGG C^C[AT][AT]GG
1058 CCAAGG
Fnu4HI 451 GCAGC GC^[ACGT]GC
1324 GCTGC
FokI 1174 CATCC GGATG(9/13)
1155 GGATG
HinfI 1648 GAGTC G^A[ACGT]TC
1663 GAGTC
HphI 661 TCACC GGTGA(8/7)
1350 TCACC
ItaI 451 GCAGC GC^[ACGT]GC
1324 GCTGC
MslI 573 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1351 CACCCCAGTG
PleI 1648 GAGTC GAGTC(4/5)
1663 GAGTC
RcaI 53 TCATGA T^CATGA
846 TCATGA
SfaNI 1156 GATGC GCATC(5/9)
843 GCATC
StyI 18 CCTTGG C^C[AT][AT]GG
1058 CCAAGG
TaqI 330 TCGA T^CGA
369 TCGA
TseI 451 GCAGC G^C[AT]GC
1324 GCTGC
TthHB8I 330 TCGA T^CGA
369 TCGA
XmnI 1165 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1557 GAAAGAATTC
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AluI AlwI ApoI BalI
BfaI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstNI BstYI DdeI
DpnII EaeI EarI EcoRII EcoRV
HaeIII HincII HindII Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MvaI NdeII
NlaIII Sau3AI ScrFI Tru9I Tsp45I
Tsp509I TspRI XhoII