Restriction map for
pFN21AE9634
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII BamHI BbeI BbrPI BcgI
BglI BlpI Bpu1102I BsaAI BsaHI
BsiEI BsiWI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
CpoI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MluI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I Tth111I XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 3223 CGCG CG^CG
AccIII 3213 TCCGGA T^CCGGA
AflII 1222 CTTAAG C^TTAAG
AflIII 16 ACATGT A^C[GA][CT]GT
AocI 2143 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3678 AGCGCT AGC^GCT
AspHI 1085 GAGCTC G[AT]GC[AT]^C
BciVI 2327 GGATAC GTATCC(6/5)
BfrI 1222 CTTAAG C^TTAAG
BseAI 3213 TCCGGA T^CCGGA
BsiHKAI 1085 GAGCTC G[AT]GC[AT]^C
BsmBI 2502 GAGACG CGTCTC(1/5)
BspEI 3213 TCCGGA T^CCGGA
BspLU11I 16 ACATGT A^CATGT
BsrFI 788 GCCGGT [GA]^CCGG[CT]
BssSI 1789 CACGAG CACGAG(-5/-1)
BstAPI 3973 GCAATGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3223 CGCG CG^CG
Bsu36I 2143 CCTGAGG CC^T[ACGT]AGG
Cfr10I 788 GCCGGT [GA]^CCGG[CT]
DraI 2709 TTTAAA TTT^AAA
DrdI 44 GACGAGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3678 AGCGCT AGC^GCT
Eco81I 2143 CCTGAGG CC^T[ACGT]AGG
EcoNI 409 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2629 ATGCAT ATGCA^T
MroI 3213 TCCGGA T^CCGGA
MspA1I 2680 CAGCTG C[AC]G^C[GT]G
MvnI 3223 CGCG CG^CG
NcoI 1277 CCATGG C^CATGG
NheI 3033 GCTAGC G^CTAGC
NsiI 2629 ATGCAT ATGCA^T
PaeR7I 2877 CTCGAG C^TCGAG
PflMI 391 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2937 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3859 CTGCAG CTGCA^G
PvuII 2680 CAGCTG CAG^CTG
SacI 1085 GAGCTC GAGCT^C
ScaI 3479 AGTACT AGT^ACT
SmaI 39 CCCGGG CCC^GGG
SspI 1840 AATATT AAT^ATT
StuI 3622 AGGCCT AGG^CCT
SwaI 2708 ATTTAAAT ATTT^AAAT
Van91I 391 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2877 CTCGAG C^TCGAG
XmaCI 39 CCCGGG C^CCGGG
XmaI 39 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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ApaI 1031 GGGCCC GGGCC^C
3870 GGGCCC
AvrII 3417 CCTAGG C^CTAGG
3444 CCTAGG
BbvCI 86 CCTCAGC CCTCAGC(-5/-2)
130 CCTCAGC
BclI 1785 TGATCA T^GATCA
2730 TGATCA
BglII 796 AGATCT A^GATCT
937 AGATCT
BlnI 3417 CCTAGG C^CTAGG
3444 CCTAGG
BsaBI 896 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2835 GATAAATATC
BsaI 1180 GAGACC GGTCTC(1/5)
2357 GAGACC
BsaWI 1721 ACCGGA [AT]^CCGG[AT]
3213 TCCGGA
BsgI 2336 CTGCAC GTGCAG(16/14)
2124 GTGCAG
BsmI 2206 GAATGC GAATGC(1/-1)
3379 GCATTC
Bsp1407I 3683 TGTACA T^GTACA
3848 TGTACA
BspDI 2260 ATCGAT AT^CGAT
3056 ATCGAT
BsrGI 3683 TGTACA T^GTACA
3848 TGTACA
BstXI 1125 CCAGAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3641 CCAAGACCTTGG
BstYI 796 AGATCT [GA]^GATC[CT]
937 AGATCT
ClaI 2260 ATCGAT AT^CGAT
3056 ATCGAT
DsaI 1277 CCATGG C^C[GA][CT]GG
1393 CCGTGG
FbaI 1785 TGATCA T^GATCA
2730 TGATCA
HaeII 3630 GGCGCT [GA]GCGC^[CT]
3678 AGCGCT
MaeII 1508 ACGT A^CGT
2049 ACGT
MamI 896 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2835 GATAAATATC
MfeI 3123 CAATTG C^AATTG
3338 CAATTG
MflI 796 AGATCT [GA]^GATC[CT]
937 AGATCT
MunI 3123 CAATTG C^AATTG
3338 CAATTG
SfcI 1298 CTACAG C^T[GA][CT]AG
3859 CTGCAG
SmlI 1222 CTTAAG C^T[CT][GA]AG
2877 CTCGAG
SphI 2574 GCATGC GCATG^C
3584 GCATGC
SspBI 3683 TGTACA T^GTACA
3848 TGTACA
XhoII 796 AGATCT [GA]^GATC[CT]
937 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmnI