Restriction map for
pFN21AE6387
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV FauI FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 340 GACGTC GACGT^C
AccII 79 CGCG CG^CG
AcyI 340 GACGTC G[GA]^CG[CT]C
ApaI 3 GGGCCC GGGCC^C
AseI 784 ATTAAT AT^TAAT
AsnI 784 ATTAAT AT^TAAT
AvaII 886 GGACC G^G[AT]CC
BalI 90 TGGCCA TGG^CCA
BamHI 695 GGATCC G^GATCC
BanII 3 GGGCCC G[GA]GC[CT]^C
BbsI 601 GAAGAC GAAGAC(2/6)
BglI 5 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 3 GGGCCC G[AGT]GC[ACT]^C
BpuAI 601 GAAGAC GAAGAC(2/6)
BsaAI 292 TACGTG [CT]AC^GT[GA]
BsaHI 340 GACGTC G[GA]^CG[CT]C
BsaWI 451 ACCGGA [AT]^CCGG[AT]
BsmI 954 GAATGC GAATGC(1/-1)
Bsp1286I 3 GGGCCC G[AGT]GC[ACT]^C
BspDI 252 ATCGAT AT^CGAT
BspMI 123 GCAGGT ACCTGC(4/8)
Bst1107I 67 GTATAC GTA^TAC
BstEII 447 GGTGACC G^GT[ACGT]ACC
BstPI 447 GGTGACC G^GT[ACGT]ACC
BstUI 79 CGCG CG^CG
BstZ17I 67 GTATAC GTA^TAC
CfoI 111 GCGC GCG^C
ClaI 252 ATCGAT AT^CGAT
DdeI 48 CTCAG C^T[ACGT]AG
EaeI 90 TGGCCA [CT]^GGCC[GA]
EcoO65I 447 GGTGACC G^GT[ACGT]ACC
EcoT14I 118 CCTTGG C^C[AT][AT]GG
EcoT22I 956 ATGCAT ATGCA^T
HaeII 110 GGCGCT [GA]GCGC^[CT]
HapII 452 CCGG C^CGG
HhaI 111 GCGC GCG^C
Hin1I 340 GACGTC G[GA]^CG[CT]C
HinP1I 111 GCGC G^CGC
HpaII 452 CCGG C^CGG
MluNI 90 TGGCCA TGG^CCA
MscI 90 TGGCCA TGG^CCA
MslI 679 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 452 CCGG C^CGG
MvnI 79 CGCG CG^CG
NsiI 956 ATGCAT ATGCA^T
PshBI 784 ATTAAT AT^TAAT
PstI 104 CTGCAG CTGCA^G
ScaI 287 AGTACT AGT^ACT
SfaNI 97 GATGC GCATC(5/9)
StyI 118 CCTTGG C^C[AT][AT]GG
TaqI 253 TCGA T^CGA
TfiI 655 GATTC G^A[AT]TC
Tsp509I 429 AATT ^AATT
TthHB8I 253 TCGA T^CGA
XbaI 813 TCTAGA T^CTAGA
XcmI 433 CCACCCTTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 67 GTATAC GT^[AC][GT]AC
751 GTCTAC
AciI 78 CCGC CCGC(-3/-1)
531 CCGC
AflIII 279 ACGTGT A^C[GA][CT]GT
293 ACGTGT
BfaI 247 CTAG C^TAG
814 CTAG
BsiYI 33 CCTTCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
437 CCTTTTGTTGG
BslI 33 CCTTCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
437 CCTTTTGTTGG
BsmFI 869 GTCCC GGGAC(10/14)
971 GTCCC
BstYI 44 GGATCT [GA]^GATC[CT]
695 GGATCC
DraII 3 GGGCCCT [GA]G^G[ACGT]CC[CT]
663 AGGGCCT
EarI 740 CTCTTC CTCTTC(1/4)
949 GAAGAG
EcoO109I 3 GGGCCCT [GA]G^G[ACGT]CC[CT]
663 AGGGCCT
Ksp632I 740 CTCTTC CTCTTC(1/4)
949 GAAGAG
MaeI 247 CTAG C^TAG
814 CTAG
MflI 44 GGATCT [GA]^GATC[CT]
695 GGATCC
MseI 467 TTAA T^TAA
785 TTAA
NlaIII 633 CATG CATG^
959 CATG
PleI 268 GACTC GAGTC(4/5)
749 GAGTC
SfcI 104 CTGCAG C^T[GA][CT]AG
914 CTGTAG
SmlI 270 CTCAAG C^T[CT][GA]AG
365 CTCAAG
Tru9I 467 TTAA T^TAA
785 TTAA
XhoII 44 GGATCT [GA]^GATC[CT]
695 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI AlwI BbvI BpmI
BsaJI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIV RsaI
Sau3AI Sau96I ScrFI TseI Tsp45I
TspRI