Restriction map for
pFN21AE3675
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# NO CUT ENZYMES ( 167 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1038 GACGTC GACGT^C
AlwNI 1061 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 577 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 37 GGCACC G^G[CT][GA]CC
BbsI 823 GTCTTC GAAGAC(2/6)
BcnI 177 CCGGG CC^[GC]GG
BmyI 36 GGGCAC G[AGT]GC[ACT]^C
BpmI 855 CTCCAG CTGGAG(16/14)
BpuAI 823 GTCTTC GAAGAC(2/6)
BsaJI 497 CCTGGG C^C[ACGT][ACGT]GG
BseRI 412 GAGGAG GAGGAG(10/8)
BsmAI 638 GAGAC GTCTC(1/5)
Bsp1286I 36 GGGCAC G[AGT]GC[ACT]^C
BspHI 363 TCATGA T^CATGA
BspMI 468 ACCTGC ACCTGC(4/8)
BsrDI 258 CATTGC GCAATG(2/0)
BsrI 857 CCAGT ACTGG(1/-1)
BstXI 743 CCATGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 372 TTTAAA TTT^AAA
EarI 844 CTCTTC CTCTTC(1/4)
EcoNI 405 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1024 GAATTC G^AATTC
HgaI 284 GCGTC GACGC(5/10)
HinfI 1063 GAATC G^A[ACGT]TC
HphI 494 TCACC GGTGA(8/7)
Ksp632I 844 CTCTTC CTCTTC(1/4)
MslI 945 CACTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 787 CAGCTG C[AC]G^C[GT]G
NciI 177 CCGGG CC^[GC]GG
PshAI 64 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 696 CTGCAG CTGCA^G
PvuII 787 CAGCTG CAG^CTG
RcaI 363 TCATGA T^CATGA
SapI 843 GCTCTTC GCTCTTC(1/4)
SfcI 696 CTGCAG C^T[GA][CT]AG
SmlI 1010 CTCAAG C^T[CT][GA]AG
SspI 677 AATATT AAT^ATT
TaqI 540 TCGA T^CGA
TfiI 1063 GAATC G^A[AT]TC
Tsp45I 959 GTCAC ^GT[GC]AC
TthHB8I 540 TCGA T^CGA
XmnI 577 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 283 GGCGTC G[GA]^CG[CT]C
1038 GACGTC
AfaI 159 GTAC GT^AC
777 GTAC
BsaHI 283 GGCGTC G[GA]^CG[CT]C
1038 GACGTC
BsgI 757 CTGCAC GTGCAG(16/14)
790 CTGCAC
DdeI 316 CTGAG C^T[ACGT]AG
634 CTAAG
DpnII 331 GATC ^GATC
747 GATC
HapII 177 CCGG C^CGG
840 CCGG
Hin1I 283 GGCGTC G[GA]^CG[CT]C
1038 GACGTC
HpaII 177 CCGG C^CGG
840 CCGG
MaeII 232 ACGT A^CGT
1039 ACGT
MaeIII 137 GTAAC ^GT[ACGT]AC
959 GTCAC
MboI 331 GATC ^GATC
747 GATC
MseI 373 TTAA T^TAA
445 TTAA
MspI 177 CCGG C^CGG
840 CCGG
MwoI 193 GCATACAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
759 GCACCTAAAGC
NdeII 331 GATC ^GATC
747 GATC
NlaIV 37 GGCACC GG[ACGT]^[ACGT]CC
500 GGGTCC
RsaI 159 GTAC GT^AC
777 GTAC
Sau3AI 331 GATC ^GATC
747 GATC
Tru9I 373 TTAA T^TAA
445 TTAA
XcmI 23 CCATCTGTAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
487 CCAGAACTCACCTGG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BsiYI BslI BssKI BstF5I
BstNI Cfr13I EcoRII Fnu4HI FokI
HaeIII ItaI MboII MnlI MvaI
NlaIII NspI Sau96I ScrFI SfaNI
TseI Tsp509I TspRI