Restriction map for
pFN21AE3340
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AflII AflIII AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BciVI BfrI BlnI
BsiWI BspDI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI DraI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoRI EcoRV FseI FspI HindIII
HpaI KasI KpnI KspI MluI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI PvuI SacI
SacII SalI SapI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspI StuI SwaI XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 1005 GACGTC GACGT^C
AccI 409 GTAGAC GT^[AC][GT]AC
AccII 1168 CGCG CG^CG
AccIII 2645 TCCGGA T^CCGGA
AclI 60 AACGTT AA^CGTT
AcsI 603 AAATTT [GA]^AATT[CT]
AgeI 913 ACCGGT A^CCGGT
AocI 2124 CCTCAGG CC^T[ACGT]AGG
ApoI 603 AAATTT [GA]^AATT[CT]
AspHI 642 GAGCAC G[AT]GC[AT]^C
AspI 352 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 662 CTCGGG C^[CT]CG[GA]G
BbvCI 1602 GCTGAGG CCTCAGC(-5/-2)
BclI 2081 TGATCA T^GATCA
BglI 744 GCCTATTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1775 AGATCT A^GATCT
BlpI 2757 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2757 GCTCAGC GC^T[ACGT]AGC
BsaAI 1939 TACGTG [CT]AC^GT[GA]
BsaBI 1770 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2645 TCCGGA T^CCGGA
BsgI 900 CTGCAC GTGCAG(16/14)
BsiHKAI 642 GAGCAC G[AT]GC[AT]^C
BsmI 2903 GCATTC GAATGC(1/-1)
BsoBI 662 CTCGGG C^[CT]CG[GA]G
BspEI 2645 TCCGGA T^CCGGA
BspHI 2078 TCATGA T^CATGA
BspMI 760 ACCTGC ACCTGC(4/8)
BstUI 1168 CGCG CG^CG
Bsu36I 2124 CCTCAGG CC^T[ACGT]AGG
CelII 2757 GCTCAGC GC^T[ACGT]AGC
CpoI 910 CGGACCG CG^G[AT]CCG
EagI 1640 CGGCCG C^GGCCG
EclXI 1640 CGGCCG C^GGCCG
Eco52I 1640 CGGCCG C^GGCCG
Eco81I 2124 CCTCAGG CC^T[ACGT]AGG
EcoT22I 672 ATGCAT ATGCA^T
FbaI 2081 TGATCA T^GATCA
HaeII 2761 AGCGCC [GA]GCGC^[CT]
MamI 1770 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 207 CAATTG C^AATTG
MroI 2645 TCCGGA T^CCGGA
MseI 64 TTAA T^TAA
MunI 207 CAATTG C^AATTG
MvnI 1168 CGCG CG^CG
NaeI 2600 GCCGGC GCC^GGC
NgoMIV 2600 GCCGGC G^CCGGC
NsiI 672 ATGCAT ATGCA^T
PflFI 352 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2157 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 913 ACCGGT A^CCGGT
Psp1406I 60 AACGTT AA^CGTT
RcaI 2078 TCATGA T^CATGA
RsrII 910 CGGACCG CG^G[AT]CCG
SbfI 811 CCTGCAGG CCTGCA^GG
SmlI 2662 CTCAAG C^T[CT][GA]AG
Sse8387I 811 CCTGCAGG CCTGCA^GG
TfiI 2116 GATTC G^A[AT]TC
Tru9I 64 TTAA T^TAA
Tth111I 352 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2157 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 888 GGCGTC G[GA]^CG[CT]C
1005 GACGTC
ApaI 242 GGGCCC GGGCC^C
1190 GGGCCC
BamHI 310 GGATCC G^GATCC
1803 GGATCC
BanI 741 GGTGCC G^G[CT][GA]CC
1025 GGCACC
BsaHI 888 GGCGTC G[GA]^CG[CT]C
1005 GACGTC
BsaI 322 GAGACC GGTCTC(1/5)
2466 GAGACC
BsiEI 1640 CGGCCG CG[GA][CT]^CG
2709 CGACCG
Bsp1407I 1721 TGTACA T^GTACA
1898 TGTACA
BsrGI 1721 TGTACA T^GTACA
1898 TGTACA
BstEII 2058 GGTCACC G^GT[ACGT]ACC
2826 GGTCACC
BstPI 2058 GGTCACC G^GT[ACGT]ACC
2826 GGTCACC
CfoI 267 GCGC GCG^C
2762 GCGC
EarI 554 CTCTTC CTCTTC(1/4)
1864 GAAGAG
EcoO65I 2058 GGTCACC G^GT[ACGT]ACC
2826 GGTCACC
HhaI 267 GCGC GCG^C
2762 GCGC
Hin1I 888 GGCGTC G[GA]^CG[CT]C
1005 GACGTC
HinP1I 267 GCGC G^CGC
2762 GCGC
Ksp632I 554 CTCTTC CTCTTC(1/4)
1864 GAAGAG
NcoI 1445 CCATGG C^CATGG
2537 CCATGG
PpuMI 972 AGGTCCC [GA]G^G[AT]CC[CT]
2228 GGGACCT
SexAI 173 ACCAGGT A^CC[AT]GGT
2264 ACCTGGT
SphI 796 GCATGC GCATG^C
952 GCATGC
SspBI 1721 TGTACA T^GTACA
1898 TGTACA
XcmI 441 CCAGGTGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1218 CCAGGCCATCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanII BbsI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII