################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## Acc65I AflII AflIII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbeI BbrPI BciVI BfrI BlnI BsiWI BspDI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I ClaI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoRI EcoRV FseI FspI HindIII HpaI KasI KpnI KspI MluI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI PvuI SacI SacII SalI SapI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 1005 GACGTC GACGT^C AccI 409 GTAGAC GT^[AC][GT]AC AccII 1168 CGCG CG^CG AccIII 2645 TCCGGA T^CCGGA AclI 60 AACGTT AA^CGTT AcsI 603 AAATTT [GA]^AATT[CT] AgeI 913 ACCGGT A^CCGGT AocI 2124 CCTCAGG CC^T[ACGT]AGG ApoI 603 AAATTT [GA]^AATT[CT] AspHI 642 GAGCAC G[AT]GC[AT]^C AspI 352 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 662 CTCGGG C^[CT]CG[GA]G BbvCI 1602 GCTGAGG CCTCAGC(-5/-2) BclI 2081 TGATCA T^GATCA BglI 744 GCCTATTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1775 AGATCT A^GATCT BlpI 2757 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2757 GCTCAGC GC^T[ACGT]AGC BsaAI 1939 TACGTG [CT]AC^GT[GA] BsaBI 1770 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2645 TCCGGA T^CCGGA BsgI 900 CTGCAC GTGCAG(16/14) BsiHKAI 642 GAGCAC G[AT]GC[AT]^C BsmI 2903 GCATTC GAATGC(1/-1) BsoBI 662 CTCGGG C^[CT]CG[GA]G BspEI 2645 TCCGGA T^CCGGA BspHI 2078 TCATGA T^CATGA BspMI 760 ACCTGC ACCTGC(4/8) BstUI 1168 CGCG CG^CG Bsu36I 2124 CCTCAGG CC^T[ACGT]AGG CelII 2757 GCTCAGC GC^T[ACGT]AGC CpoI 910 CGGACCG CG^G[AT]CCG EagI 1640 CGGCCG C^GGCCG EclXI 1640 CGGCCG C^GGCCG Eco52I 1640 CGGCCG C^GGCCG Eco81I 2124 CCTCAGG CC^T[ACGT]AGG EcoT22I 672 ATGCAT ATGCA^T FbaI 2081 TGATCA T^GATCA HaeII 2761 AGCGCC [GA]GCGC^[CT] MamI 1770 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 207 CAATTG C^AATTG MroI 2645 TCCGGA T^CCGGA MseI 64 TTAA T^TAA MunI 207 CAATTG C^AATTG MvnI 1168 CGCG CG^CG NaeI 2600 GCCGGC GCC^GGC NgoMIV 2600 GCCGGC G^CCGGC NsiI 672 ATGCAT ATGCA^T PflFI 352 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2157 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 913 ACCGGT A^CCGGT Psp1406I 60 AACGTT AA^CGTT RcaI 2078 TCATGA T^CATGA RsrII 910 CGGACCG CG^G[AT]CCG SbfI 811 CCTGCAGG CCTGCA^GG SmlI 2662 CTCAAG C^T[CT][GA]AG Sse8387I 811 CCTGCAGG CCTGCA^GG TfiI 2116 GATTC G^A[AT]TC Tru9I 64 TTAA T^TAA Tth111I 352 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2157 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 888 GGCGTC G[GA]^CG[CT]C 1005 GACGTC ApaI 242 GGGCCC GGGCC^C 1190 GGGCCC BamHI 310 GGATCC G^GATCC 1803 GGATCC BanI 741 GGTGCC G^G[CT][GA]CC 1025 GGCACC BsaHI 888 GGCGTC G[GA]^CG[CT]C 1005 GACGTC BsaI 322 GAGACC GGTCTC(1/5) 2466 GAGACC BsiEI 1640 CGGCCG CG[GA][CT]^CG 2709 CGACCG Bsp1407I 1721 TGTACA T^GTACA 1898 TGTACA BsrGI 1721 TGTACA T^GTACA 1898 TGTACA BstEII 2058 GGTCACC G^GT[ACGT]ACC 2826 GGTCACC BstPI 2058 GGTCACC G^GT[ACGT]ACC 2826 GGTCACC CfoI 267 GCGC GCG^C 2762 GCGC EarI 554 CTCTTC CTCTTC(1/4) 1864 GAAGAG EcoO65I 2058 GGTCACC G^GT[ACGT]ACC 2826 GGTCACC HhaI 267 GCGC GCG^C 2762 GCGC Hin1I 888 GGCGTC G[GA]^CG[CT]C 1005 GACGTC HinP1I 267 GCGC G^CGC 2762 GCGC Ksp632I 554 CTCTTC CTCTTC(1/4) 1864 GAAGAG NcoI 1445 CCATGG C^CATGG 2537 CCATGG PpuMI 972 AGGTCCC [GA]G^G[AT]CC[CT] 2228 GGGACCT SexAI 173 ACCAGGT A^CC[AT]GGT 2264 ACCTGGT SphI 796 GCATGC GCATG^C 952 GCATGC SspBI 1721 TGTACA T^GTACA 1898 TGTACA XcmI 441 CCAGGTGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1218 CCAGGCCATCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BalI BanII BbsI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII