##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BssSI       Bst1107I    BstAPI      
BstEII      BstF5I      BstNI       BstPI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
DsaI        Eam1105I    EarI        Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HapII       HindIII     
HinfI       HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeI        MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MspA1I      MspI        MunI        
MvaI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AcyI           82  GGCGTC               G[GA]^CG[CT]C
AfaI           30  GTAC                 GT^AC
AluI          152  AGCT                 AG^CT
AvaII          50  GGACC                G^G[AT]CC
BsaHI          82  GGCGTC               G[GA]^CG[CT]C
BsiEI           5  CGGCCG               CG[GA][CT]^CG
BssHII         99  GCGCGC               G^CGCGC
BstBI          21  TTCGAA               TT^CGAA
BstXI          71  CCATCATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          99  GCGCGC               GC[ACGT]^[ACGT]GC
Cfr13I         50  GGACC                G^G[ACGT]CC
DpnII         109  GATC                 ^GATC
DraII          49  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI            5  CGGCCG               [CT]^GGCC[GA]
EagI            5  CGGCCG               C^GGCCG
EclXI           5  CGGCCG               C^GGCCG
Eco52I          5  CGGCCG               C^GGCCG
EcoO109I       49  AGGACCT              [GA]G^G[ACGT]CC[CT]
HaeIII          6  GGCC                 GG^CC
Hin1I          82  GGCGTC               G[GA]^CG[CT]C
HincII         15  GTTGAC               GT[CT]^[GA]AC
HindII         15  GTTGAC               GT[CT]^[GA]AC
HphI           68  TCACC                GGTGA(8/7)
MaeII          13  ACGT                 A^CGT
MboI          109  GATC                 ^GATC
MboII          92  TCTTC                GAAGA(8/7)
NdeII         109  GATC                 ^GATC
NlaIII         87  CATG                 CATG^
NotI            4  GCGGCCGC             GC^GGCCGC
NspV           21  TTCGAA               TT^CGAA
PpuMI          49  AGGACCT              [GA]G^G[AT]CC[CT]
RsaI           30  GTAC                 GT^AC
Sau3AI        109  GATC                 ^GATC
Sau96I         50  GGACC                G^G[ACGT]CC
SfuI           21  TTCGAA               TT^CGAA


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

Fnu4HI          4  GCGGC                GC^[ACGT]GC
                7  GCCGC               
HgaI          121  GACGC                GACGC(5/10)
               83  GCGTC               
ItaI            4  GCGGC                GC^[ACGT]GC
                7  GCCGC               
MslI           72  CATCATCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              172  CACAAGTATG          


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AccII       AciI        BstUI       CfoI        HhaI        
HinP1I      MnlI        MvnI        TaqI        TthHB8I