Restriction map for
pFN21AE4145
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NcoI NdeI
NheI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 100 GACGTC GACGT^C
AccI 1447 GTCTAC GT^[AC][GT]AC
AflIII 1451 ACGTGT A^C[GA][CT]GT
AhdI 1018 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 664 GTGCAC G^TGCAC
AlwI 723 GATCC GGATC(4/5)
ApaLI 664 GTGCAC G^TGCAC
AspEI 1018 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 1681 GTCTTC GAAGAC(2/6)
BfaI 661 CTAG C^TAG
BglI 1111 GCCAGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1133 AGATCT A^GATCT
BpuAI 1681 GTCTTC GAAGAC(2/6)
BsmAI 1693 GTCTC GTCTC(1/5)
Bsp1407I 272 TGTACA T^GTACA
BsrBI 529 GAGCGG CCGCTC(-3/-3)
BsrGI 272 TGTACA T^GTACA
BstXI 398 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1133 AGATCT [GA]^GATC[CT]
DraIII 1529 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1018 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1026 GTCAAC GT[CT]^[GA]AC
HindII 1026 GTCAAC GT[CT]^[GA]AC
MaeI 661 CTAG C^TAG
MboII 1682 TCTTC GAAGA(8/7)
MflI 1133 AGATCT [GA]^GATC[CT]
PaeR7I 589 CTCGAG C^TCGAG
PpuMI 806 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1206 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1200 CAGCTG CAG^CTG
SacI 1414 GAGCTC GAGCT^C
SmlI 589 CTCGAG C^T[CT][GA]AG
SspBI 272 TGTACA T^GTACA
TaqI 590 TCGA T^CGA
TthHB8I 590 TCGA T^CGA
XhoI 589 CTCGAG C^TCGAG
XhoII 1133 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbeI 1080 GGCGCC GGCGC^C
1639 GGCGCC
BbrPI 497 CACGTG CAC^GTG
1588 CACGTG
BlpI 156 GCTCAGC GC^T[ACGT]AGC
654 GCTCAGC
Bpu1102I 156 GCTCAGC GC^T[ACGT]AGC
654 GCTCAGC
CelII 156 GCTCAGC GC^T[ACGT]AGC
654 GCTCAGC
EcoT14I 455 CCAAGG C^C[AT][AT]GG
873 CCTTGG
HgaI 673 GACGC GACGC(5/10)
1206 GACGC
HinfI 587 GACTC G^A[ACGT]TC
730 GAGTC
KasI 1080 GGCGCC G^GCGCC
1639 GGCGCC
MspA1I 1200 CAGCTG C[AC]G^C[GT]G
1560 CCGCTG
NarI 1080 GGCGCC GG^CGCC
1639 GGCGCC
NotI 365 GCGGCCGC GC^GGCCGC
1556 GCGGCCGC
PleI 587 GACTC GAGTC(4/5)
730 GAGTC
PmaCI 497 CACGTG CAC^GTG
1588 CACGTG
PmlI 497 CACGTG CAC^GTG
1588 CACGTG
SexAI 1021 ACCTGGT A^CC[AT]GGT
1274 ACCTGGT
SfoI 1080 GGCGCC GGC^GCC
1639 GGCGCC
SmaI 629 CCCGGG CCC^GGG
1462 CCCGGG
SphI 42 GCATGC GCATG^C
739 GCATGC
StyI 455 CCAAGG C^C[AT][AT]GG
873 CCTTGG
XmaCI 629 CCCGGG C^CCGGG
1462 CCCGGG
XmaI 629 CCCGGG C^CCGGG
1462 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MnlI MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI TseI Tsp45I TspRI
Van91I XcmI