Restriction map for
pFN21AE2325
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AvaI AviII
BbeI BbrPI BciVI BclI BglI
BsaHI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BssHII
Bst1107I BstAPI BstZ17I Bsu36I CfoI
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 2731 CGCG CG^CG
AflII 5679 CTTAAG C^TTAAG
AgeI 3326 ACCGGT A^CCGGT
Alw44I 399 GTGCAC G^TGCAC
ApaLI 399 GTGCAC G^TGCAC
AseI 4990 ATTAAT AT^TAAT
AsnI 4990 ATTAAT AT^TAAT
AvrII 618 CCTAGG C^CTAGG
BanI 3586 GGTGCC G^G[CT][GA]CC
BcgI 4071 CGAATGTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 202 CCCGG CC^[GC]GG
BfrI 5679 CTTAAG C^TTAAG
BlnI 618 CCTAGG C^CTAGG
BsaAI 2984 TACGTG [CT]AC^GT[GA]
BsaBI 2532 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 3345 GTGCAG GTGCAG(16/14)
Bsp1407I 5228 TGTACA T^GTACA
BsrGI 5228 TGTACA T^GTACA
BssSI 1023 CACGAG CACGAG(-5/-1)
BstUI 2731 CGCG CG^CG
BstXI 2098 CCACCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2232 TTTAAA TTT^AAA
DrdI 2087 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 441 CCCGC CCCGC(4/6)
HpaI 3469 GTTAAC GTT^AAC
MamI 2532 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2731 CGCG CG^CG
NciI 202 CCCGG CC^[GC]GG
NheI 5076 GCTAGC G^CTAGC
PinAI 3326 ACCGGT A^CCGGT
PshBI 4990 ATTAAT AT^TAAT
ScaI 5275 AGTACT AGT^ACT
SexAI 2834 ACCTGGT A^CC[AT]GGT
SspBI 5228 TGTACA T^GTACA
XbaI 3871 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1587 GTCTAC GT^[AC][GT]AC
4228 GTAGAC
BalI 2512 TGGCCA TGG^CCA
2585 TGGCCA
BbvCI 1309 GCTGAGG CCTCAGC(-5/-2)
3274 GCTGAGG
BlpI 1901 GCTCAGC GC^T[ACGT]AGC
3576 GCTCAGC
Bpu1102I 1901 GCTCAGC GC^T[ACGT]AGC
3576 GCTCAGC
BsaWI 3326 ACCGGT [AT]^CCGG[AT]
5468 ACCGGA
BseRI 3336 CTCCTC GAGGAG(10/8)
5399 CTCCTC
BspMI 309 ACCTGC ACCTGC(4/8)
3517 ACCTGC
BsrFI 1836 GCCGGT [GA]^CCGG[CT]
3326 ACCGGT
BstBI 638 TTCGAA TT^CGAA
827 TTCGAA
CelII 1901 GCTCAGC GC^T[ACGT]AGC
3576 GCTCAGC
Cfr10I 1836 GCCGGT [GA]^CCGG[CT]
3326 ACCGGT
DraII 21 AGGACCT [GA]G^G[ACGT]CC[CT]
1819 AGGTCCC
EcoO109I 21 AGGACCT [GA]G^G[ACGT]CC[CT]
1819 AGGTCCC
HincII 3469 GTTAAC GT[CT]^[GA]AC
4586 GTCAAC
HindII 3469 GTTAAC GT[CT]^[GA]AC
4586 GTCAAC
MluNI 2512 TGGCCA TGG^CCA
2585 TGGCCA
MscI 2512 TGGCCA TGG^CCA
2585 TGGCCA
NspV 638 TTCGAA TT^CGAA
827 TTCGAA
PflMI 3731 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5164 CCATCTGTTGG
PpuMI 21 AGGACCT [GA]G^G[AT]CC[CT]
1819 AGGTCCC
SapI 634 GCTCTTC GCTCTTC(1/4)
4917 GCTCTTC
SfuI 638 TTCGAA TT^CGAA
827 TTCGAA
SphI 386 GCATGC GCATG^C
2618 GCATGC
StuI 687 AGGCCT AGG^CCT
3422 AGGCCT
Van91I 3731 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5164 CCATCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BamHI BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspLU11I BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoNI EcoO65I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI