Restriction map for
pFN21AE2544
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
BbeI BbrPI BciVI BfrI BglI
BlpI BpmI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI BsoBI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstZ17I
Bsu36I CelII CfoI Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 272 GGTACC G^GTACC
AccI 366 GTCGAC GT^[AC][GT]AC
AccII 1171 CGCG CG^CG
AciI 407 CCGC CCGC(-3/-1)
Asp718I 272 GGTACC G^GTACC
AvrII 4 CCTAGG C^CTAGG
BanII 1289 GAGCTC G[GA]GC[CT]^C
BbvCI 1435 GCTGAGG CCTCAGC(-5/-2)
BcgI 1557 GCATGATATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2561 TGATCA T^GATCA
BcnI 1635 CCGGG CC^[GC]GG
BglII 2658 AGATCT A^GATCT
BlnI 4 CCTAGG C^CTAGG
BsaBI 2226 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 397 GGTCTC GGTCTC(1/5)
BsaWI 2222 ACCGGA [AT]^CCGG[AT]
BsmBI 263 CGTCTC CGTCTC(1/5)
BsmI 1187 GCATTC GAATGC(1/-1)
Bsp1407I 1821 TGTACA T^GTACA
BspDI 1693 ATCGAT AT^CGAT
BsrDI 1875 GCAATG GCAATG(2/0)
BsrGI 1821 TGTACA T^GTACA
BssSI 1810 CTCGTG CACGAG(-5/-1)
BstBI 1540 TTCGAA TT^CGAA
BstUI 1171 CGCG CG^CG
ClaI 1693 ATCGAT AT^CGAT
DraII 1256 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 1256 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 1687 GATATC GAT^ATC
FbaI 2561 TGATCA T^GATCA
HpaI 1842 GTTAAC GTT^AAC
KpnI 272 GGTACC GGTAC^C
MaeII 2925 ACGT A^CGT
MamI 2226 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 206 CAATTG C^AATTG
MunI 206 CAATTG C^AATTG
MvnI 1171 CGCG CG^CG
NciI 1635 CCGGG CC^[GC]GG
NcoI 908 CCATGG C^CATGG
NspV 1540 TTCGAA TT^CGAA
PflMI 3064 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1256 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1289 GAGCTC GAGCT^C
SalI 366 GTCGAC G^TCGAC
SapI 586 GAAGAGC GCTCTTC(1/4)
SfuI 1540 TTCGAA TT^CGAA
SphI 1046 GCATGC GCATG^C
SspBI 1821 TGTACA T^GTACA
SspI 1321 AATATT AAT^ATT
StuI 580 AGGCCT AGG^CCT
Van91I 3064 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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Asp700I 1109 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1423 GAAAAAATTC
AspHI 1289 GAGCTC G[AT]GC[AT]^C
1401 GAGCAC
BanI 272 GGTACC G^G[CT][GA]CC
904 GGTGCC
BmyI 1289 GAGCTC G[AGT]GC[ACT]^C
1401 GAGCAC
BseRI 1937 CTCCTC GAGGAG(10/8)
3077 GAGGAG
BsiHKAI 1289 GAGCTC G[AT]GC[AT]^C
1401 GAGCAC
Bsp1286I 1289 GAGCTC G[AGT]GC[ACT]^C
1401 GAGCAC
DsaI 908 CCATGG C^C[GA][CT]GG
2581 CCACGG
HapII 1635 CCGG C^CGG
2223 CCGG
HincII 366 GTCGAC GT[CT]^[GA]AC
1842 GTTAAC
HindII 366 GTCGAC GT[CT]^[GA]AC
1842 GTTAAC
HpaII 1635 CCGG C^CGG
2223 CCGG
MspI 1635 CCGG C^CGG
2223 CCGG
SmlI 1388 CTCAAG C^T[CT][GA]AG
1940 CTCAAG
XmnI 1109 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1423 GAAAAAATTC
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BalI BamHI BbsI
BbvI BfaI BpuAI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII