Restriction map for
pFN21AB9930
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BseAI BsiWI BsoBI BspDI
BspEI BspHI BspMI BsrDI BssHII
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoRI EcoT22I FbaI
FspI Hin1I HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI MvnI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 764 GGTACC G^GTACC
AcsI 75 GAATTT [GA]^AATT[CT]
AflIII 1046 ACATGT A^C[GA][CT]GT
AlwI 1257 GATCC GGATC(4/5)
AlwNI 505 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 75 GAATTT [GA]^AATT[CT]
Asp700I 705 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 764 GGTACC G^GTACC
BalI 819 TGGCCA TGG^CCA
BanI 764 GGTACC G^G[CT][GA]CC
BbvCI 1362 GCTGAGG CCTCAGC(-5/-2)
BcnI 1139 CCCGG CC^[GC]GG
BfaI 1094 CTAG C^TAG
BsaWI 761 TCCGGT [AT]^CCGG[AT]
BsiEI 1530 CGGCCG CG[GA][CT]^CG
BsmFI 234 GTCCC GGGAC(10/14)
BsmI 864 GCATTC GAATGC(1/-1)
Bsp1407I 129 TGTACA T^GTACA
BspLU11I 1046 ACATGT A^CATGT
BsrBI 1605 GAGCGG CCGCTC(-3/-3)
BsrGI 129 TGTACA T^GTACA
BstEII 1499 GGTCACC G^GT[ACGT]ACC
BstNI 1284 CCTGG CC^[AT]GG
BstPI 1499 GGTCACC G^GT[ACGT]ACC
BstXI 1337 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1256 AGATCC [GA]^GATC[CT]
EagI 1530 CGGCCG C^GGCCG
EclXI 1530 CGGCCG C^GGCCG
Eco52I 1530 CGGCCG C^GGCCG
EcoO65I 1499 GGTCACC G^GT[ACGT]ACC
EcoRII 1284 CCTGG ^CC[AT]GG
EcoRV 160 GATATC GAT^ATC
FseI 1531 GGCCGGCC GGCCGG^CC
HaeII 281 AGCGCC [GA]GCGC^[CT]
HgaI 1275 GCGTC GACGC(5/10)
HincII 378 GTTGAC GT[CT]^[GA]AC
HindII 378 GTTGAC GT[CT]^[GA]AC
KpnI 764 GGTACC GGTAC^C
MaeI 1094 CTAG C^TAG
MflI 1256 AGATCC [GA]^GATC[CT]
MluNI 819 TGGCCA TGG^CCA
MscI 819 TGGCCA TGG^CCA
MvaI 1284 CCTGG CC^[AT]GG
NciI 1139 CCCGG CC^[GC]GG
NdeI 353 CATATG CA^TATG
NspI 1046 ACATGT [GA]CATG^[CT]
SmlI 1185 CTTGAG C^T[CT][GA]AG
SspBI 129 TGTACA T^GTACA
TaqI 242 TCGA T^CGA
TthHB8I 242 TCGA T^CGA
XhoII 1256 AGATCC [GA]^GATC[CT]
XmnI 705 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AvaII 210 GGACC G^G[AT]CC
1431 GGTCC
BbsI 27 GAAGAC GAAGAC(2/6)
709 GTCTTC
BglI 821 GCCATGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1138 GCCCGGCTGGC
BlpI 894 GCTGAGC GC^T[ACGT]AGC
1602 GCTGAGC
BpmI 1055 CTGGAG CTGGAG(16/14)
1384 CTGGAG
Bpu1102I 894 GCTGAGC GC^T[ACGT]AGC
1602 GCTGAGC
BpuAI 27 GAAGAC GAAGAC(2/6)
709 GTCTTC
BsgI 1624 GTGCAG GTGCAG(16/14)
1655 GTGCAG
BsmBI 1276 CGTCTC CGTCTC(1/5)
1619 GAGACG
BssKI 1139 CCCGG ^CC[ACGT]GG
1284 CCTGG
BssSI 933 CACGAG CACGAG(-5/-1)
1291 CACGAG
CelII 894 GCTGAGC GC^T[ACGT]AGC
1602 GCTGAGC
CfoI 282 GCGC GCG^C
1554 GCGC
DpnII 225 GATC ^GATC
1257 GATC
HhaI 282 GCGC GCG^C
1554 GCGC
HinP1I 282 GCGC G^CGC
1554 GCGC
HphI 297 TCACC GGTGA(8/7)
1501 TCACC
MboI 225 GATC ^GATC
1257 GATC
NdeII 225 GATC ^GATC
1257 GATC
NlaIV 764 GGTACC GG[ACGT]^[ACGT]CC
1570 GGCTCC
PflMI 553 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
714 CCATCCCGTGG
SapI 1441 GAAGAGC GCTCTTC(1/4)
1468 GAAGAGC
Sau3AI 225 GATC ^GATC
1257 GATC
SbfI 1260 CCTGCAGG CCTGCA^GG
1404 CCTGCAGG
ScrFI 1139 CCCGG CC^[ACGT]GG
1284 CCTGG
Sse8387I 1260 CCTGCAGG CCTGCA^GG
1404 CCTGCAGG
StuI 1298 AGGCCT AGG^CCT
1631 AGGCCT
TfiI 469 GATTC G^A[AT]TC
1269 GATTC
Tsp45I 1165 GTGAC ^GT[GC]AC
1500 GTCAC
Van91I 553 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
714 CCATCCCGTGG
XcmI 1044 CCACATGTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1073 CCAAGAGTGTTCTGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrFI BsrI
BstF5I Cac8I Cfr10I Cfr13I DdeI
DsaI EaeI EarI EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeII MaeIII
MboII MnlI MseI MslI MspA1I
MspI MwoI NaeI NcoI NgoMIV
NlaIII PleI PstI PvuII RsaI
Sau96I SfaNI SfcI StyI Tru9I
TseI Tsp509I TspRI