Restriction map for
pFN21AE1225
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BlnI BsaAI BsaBI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DrdI DsaI Eam1105I EarI
Eco47III EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HindIII HpaI KasI KpnI Ksp632I
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcyI 749 GGCGTC G[GA]^CG[CT]C
AfaI 192 GTAC GT^AC
AocI 342 CCTGAGG CC^T[ACGT]AGG
AspHI 7 GAGCAC G[AT]GC[AT]^C
AvaII 674 GGTCC G^G[AT]CC
BbsI 337 GAAGAC GAAGAC(2/6)
BbvCI 198 GCTGAGG CCTCAGC(-5/-2)
BfaI 229 CTAG C^TAG
BglII 48 AGATCT A^GATCT
BlpI 4 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
BpuAI 337 GAAGAC GAAGAC(2/6)
BsaHI 749 GGCGTC G[GA]^CG[CT]C
BsaI 162 GGTCTC GGTCTC(1/5)
BsiEI 444 CGGCCG CG[GA][CT]^CG
BsiHKAI 7 GAGCAC G[AT]GC[AT]^C
BsmAI 163 GTCTC GTCTC(1/5)
BsmFI 629 GTCCC GGGAC(10/14)
BsrDI 678 CATTGC GCAATG(2/0)
BstYI 48 AGATCT [GA]^GATC[CT]
Bsu36I 342 CCTGAGG CC^T[ACGT]AGG
CelII 4 GCTGAGC GC^T[ACGT]AGC
CfoI 205 GCGC GCG^C
Cfr13I 674 GGTCC G^G[ACGT]CC
DraIII 640 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 444 CGGCCG [CT]^GGCC[GA]
EagI 444 CGGCCG C^GGCCG
EclXI 444 CGGCCG C^GGCCG
Eco52I 444 CGGCCG C^GGCCG
Eco81I 342 CCTGAGG CC^T[ACGT]AGG
EcoT14I 265 CCAAGG C^C[AT][AT]GG
HaeII 204 GGCGCT [GA]GCGC^[CT]
HaeIII 445 GGCC GG^CC
HapII 443 CCGG C^CGG
HhaI 205 GCGC GCG^C
Hin1I 749 GGCGTC G[GA]^CG[CT]C
HinP1I 205 GCGC G^CGC
HincII 514 GTCAAC GT[CT]^[GA]AC
HindII 514 GTCAAC GT[CT]^[GA]AC
HpaII 443 CCGG C^CGG
HphI 624 GGTGA GGTGA(8/7)
MaeI 229 CTAG C^TAG
MflI 48 AGATCT [GA]^GATC[CT]
MslI 766 CATAAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 827 CCGCTG C[AC]G^C[GT]G
MspI 443 CCGG C^CGG
RsaI 192 GTAC GT^AC
Sau96I 674 GGTCC G^G[ACGT]CC
SfaNI 794 GCATC GCATC(5/9)
StyI 265 CCAAGG C^C[AT][AT]GG
Tsp509I 106 AATT ^AATT
XhoII 48 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AciI 447 CCGC CCGC(-3/-1)
827 CCGC
BclI 786 TGATCA T^GATCA
815 TGATCA
BpmI 94 CTCCAG CTGGAG(16/14)
112 CTCCAG
BseRI 614 CTCCTC GAGGAG(10/8)
345 GAGGAG
FbaI 786 TGATCA T^GATCA
815 TGATCA
HgaI 416 GCGTC GACGC(5/10)
750 GCGTC
PleI 612 GACTC GAGTC(4/5)
627 GAGTC
TfiI 508 GAATC G^A[AT]TC
729 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI BanI BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HinfI
ItaI MaeII MboI MboII MnlI
MvaI MwoI NdeII NlaIV Sau3AI
ScrFI TaqI TseI TspRI TthHB8I