################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HpaI HphI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AatII 521 GACGTC GACGT^C AcyI 521 GACGTC G[GA]^CG[CT]C AfaI 45 GTAC GT^AC AlwI 1566 GATCC GGATC(4/5) AspHI 508 GAGCTC G[AT]GC[AT]^C AvaI 460 CCCGAG C^[CT]CG[GA]G BanI 1470 GGTGCC G^G[CT][GA]CC BfaI 1393 CTAG C^TAG BglI 736 GCCCGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 599 AGATCT A^GATCT BsaHI 521 GACGTC G[GA]^CG[CT]C BsiHKAI 508 GAGCTC G[AT]GC[AT]^C BsoBI 460 CCCGAG C^[CT]CG[GA]G BsrFI 84 GCCGGT [GA]^CCGG[CT] BstAPI 636 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 121 TTCGAA TT^CGAA BstYI 599 AGATCT [GA]^GATC[CT] Cfr10I 84 GCCGGT [GA]^CCGG[CT] HgaI 987 GACGC GACGC(5/10) Hin1I 521 GACGTC G[GA]^CG[CT]C HindIII 125 AAGCTT A^AGCTT MaeI 1393 CTAG C^TAG MflI 599 AGATCT [GA]^GATC[CT] NruI 939 TCGCGA TCG^CGA NspV 121 TTCGAA TT^CGAA RsaI 45 GTAC GT^AC SacI 508 GAGCTC GAGCT^C SfuI 121 TTCGAA TT^CGAA TfiI 714 GAATC G^A[AT]TC XhoII 599 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 940 CGCG CG^CG 1445 CGCG BbsI 448 GAAGAC GAAGAC(2/6) 684 GAAGAC BbvCI 412 GCTGAGG CCTCAGC(-5/-2) 568 GCTGAGG BlpI 486 GCTGAGC GC^T[ACGT]AGC 510 GCTCAGC Bpu1102I 486 GCTGAGC GC^T[ACGT]AGC 510 GCTCAGC BpuAI 448 GAAGAC GAAGAC(2/6) 684 GAAGAC BsmI 80 GAATGC GAATGC(1/-1) 537 GAATGC BsrBI 55 GAGCGG CCGCTC(-3/-3) 817 GAGCGG BstUI 940 CGCG CG^CG 1445 CGCG CelII 486 GCTGAGC GC^T[ACGT]AGC 510 GCTCAGC DsaI 659 CCATGG C^C[GA][CT]GG 854 CCATGG FauI 819 GCGGG CCCGC(4/6) 1449 GCGGG HincII 323 GTCAAC GT[CT]^[GA]AC 1329 GTTGAC HindII 323 GTCAAC GT[CT]^[GA]AC 1329 GTTGAC MaeII 118 ACGT A^CGT 522 ACGT MslI 395 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1319 CATCCATGTG MvnI 940 CGCG CG^CG 1445 CGCG NcoI 659 CCATGG C^CATGG 854 CCATGG PpuMI 500 AGGACCT [GA]G^G[AT]CC[CT] 677 AGGACCT XcmI 269 CCACTGTGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1633 CCACCTCCCACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AluI AlwNI AocI ApaI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I