##################################
# NO CUT ENZYMES (  218 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvI        BcgI        BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        ItaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        TseI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AluI           48  AGCT                 AG^CT
AspHI         204  GTGCTC               G[AT]GC[AT]^C
AvaII         372  GGACC                G^G[AT]CC
BanII           3  GGGCTC               G[GA]GC[CT]^C
BbvCI          44  CCTCAGC              CCTCAGC(-5/-2)
BciVI         229  GGATAC               GTATCC(6/5)
BsaJI         185  CCAAGG               C^C[ACGT][ACGT]GG
BsgI          248  GTGCAG               GTGCAG(16/14)
BsiHKAI       204  GTGCTC               G[AT]GC[AT]^C
Cac8I          49  GCTTGC               GC[ACGT]^[ACGT]GC
Cfr13I        372  GGACC                G^G[ACGT]CC
DdeI           45  CTCAG                C^T[ACGT]AG
DpnII         297  GATC                 ^GATC
DraI           69  TTTAAA               TTT^AAA
EarI           19  CTCTTC               CTCTTC(1/4)
EcoT14I       185  CCAAGG               C^C[AT][AT]GG
HgaI          290  GACGC                GACGC(5/10)
Ksp632I        19  CTCTTC               CTCTTC(1/4)
MaeIII        118  GTTAC                ^GT[ACGT]AC
MboI          297  GATC                 ^GATC
MnlI           44  CCTC                 CCTC(7/6)
NdeII         297  GATC                 ^GATC
NlaIV           4  GGCTCC               GG[ACGT]^[ACGT]CC
Sau3AI        297  GATC                 ^GATC
Sau96I        372  GGACC                G^G[ACGT]CC
StyI          185  CCAAGG               C^C[AT][AT]GG
XcmI          147  CCACTATAAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AfaI          285  GTAC                 GT^AC
              311  GTAC                
BmyI            3  GGGCTC               G[AGT]GC[ACT]^C
              204  GTGCTC              
Bsp1286I        3  GGGCTC               G[AGT]GC[ACT]^C
              204  GTGCTC              
BsrI          233  ACTGG                ACTGG(1/-1)
              302  ACTGG               
RsaI          285  GTAC                 GT^AC
              311  GTAC                
SfaNI          53  GCATC                GCATC(5/9)
              176  GCATC               
TspRI         246  CAGTG                CA[GC]TG[ACGT][ACGT]^
              350  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

AcsI        ApoI        MboII       MseI        Tru9I       
Tsp509I